Genotypes of glutathione S-transferase M1 and N-acetyltransferase 2 in Japanese patients with gastric cancer

Relationship between genetic polymorphisms of drug-metabolizing enzymes ( CYP1A1 , CYP2E1 , GSTM1 , and NAT2 ), drinking habits, histological subtypes, and p53 gene point mutations in Japanese patients with gastric cancer

“…21,23 The PCR reaction mixture was electrophoresed on a 2% agarose gel to verify the successful multiplication of the -globin gene and to identify whether or not a PCR product derived from the GSTM1 gene was present. 21,23 A set of oligonucleotide primers was used to amplify the 559-bp NAT2 genomic sequence. The 559-bp PCR product was digested by Bam HI and Asp 718.…”

“…The genotypes of the CYP2E1 gene were identified by restriction fragment length polymorphism: 20 the PCR-amplified DNA fragments, including the polymorphic site, were digested with Rsa I, and subjected to electrophoresis on 2.0% agarose gel. Identification of the GSTM1 and NAT2 genotypes was performed according to the methods of Kempkes et al, 21 Abe et al, 22 and Oda et al 23 Two sets of oligonucleotide primers were used to amplify simultaneously the polymorphic GSTM1 and a segment of -globin. 21,23 The PCR reaction mixture was electrophoresed on a 2% agarose gel to verify the successful multiplication of the -globin gene and to identify whether or not a PCR product derived from the GSTM1 gene was present.…”

“…Identification of the GSTM1 and NAT2 genotypes was performed according to the methods of Kempkes et al, 21 Abe et al, 22 and Oda et al 23 Two sets of oligonucleotide primers were used to amplify simultaneously the polymorphic GSTM1 and a segment of -globin. 21,23 The PCR reaction mixture was electrophoresed on a 2% agarose gel to verify the successful multiplication of the -globin gene and to identify whether or not a PCR product derived from the GSTM1 gene was present. 21,23 A set of oligonucleotide primers was used to amplify the 559-bp NAT2 genomic sequence.…”

Association between dietary heterocyclic amine levels, genetic polymorphisms of NAT2, CYP1A1, and CYP1A2 and risk of stomach cancer: a hospital-based case-control study in Japan

“…The NAT2 rapid acetylator phenotype is thought to be indicative of higher risk, because N-acetylation is negligible and O-acetylation is an activation step for PhIP and MeIQx association with several cancers [15,16]. However, no consistent association between the NAT2 phenotype or genotype and stomach cancer risk has been identifi ed [17][18][19][20][21][22][23]. No investigation of the combined effect of well-done meat and HCA intake with the NAT2 phenotype or genotype has appeared.…”

Multidisciplinary approach to understand the pathogenesis of gastric cancer

“…Japanese researchers suggested that a combination of GSTM1 and NAT2 decreases the risk of gastric cancer in Japanese patients [69] . A population-based case-control study in China suggested that the GSTP1 genotype seems not to be associated with the risk of gastric cancer and chronic gastritis in a high-risk Chinese population [70] .…”