Genotypic Analysis for Retrieval of MYMV Resistant Progenies from Certain Crosses of Mungbean [Vigna radiata (L.) Wilczek]

Abstract

The present investigation was conducted to study the nature of inheritance of MYMV among intra-specific crosses among three MYMV susceptible and five MYMV resistant genotypes of green gram. The molecular screening and hotspot evaluation is carried out in fifteen F1 progenies and 8 F2 families, respectively. The yellow mosaic-affected leaf samples gave positive results with MYMIV specific primer pairs YFP1/YRP1 and YFP2/YRP2 amplified fragments having size of 704bp and 648 bp, respectively. Coat protein gene sequence analysis revealed that the identified virus is homologous to MYMV. The inheritance study based on F2 data indicated the ratio of MYMV resistant plants and susceptible plants showed a good fit to expected 3:1 (Susceptible: Resistant) suggesting a typical monogenic recessive gene governing resistant reaction to MYMV besides resting the susceptibility from the susceptible parents.