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The present investigation was conducted to study the nature of inheritance of MYMV among intra-specific crosses among three MYMV susceptible and five MYMV resistant genotypes of green gram. The molecular screening and hotspot evaluation is carried out in fifteen F1 progenies and 8 F2 families, respectively. The yellow mosaic-affected leaf samples gave positive results with MYMIV specific primer pairs YFP1/YRP1 and YFP2/YRP2 amplified fragments having size of 704bp and 648 bp, respectively. Coat protein gene sequence analysis revealed that the identified virus is homologous to MYMV. The inheritance study based on F2 data indicated the ratio of MYMV resistant plants and susceptible plants showed a good fit to expected 3:1 (Susceptible: Resistant) suggesting a typical monogenic recessive gene governing resistant reaction to MYMV besides resting the susceptibility from the susceptible parents.

Twenty five diverse pumpkin germplasm from North East India were assesed for correlation and path coefficient analysis. Correlation revealed that, characters like number of fruit per plant had significant positive correlation with fruit yield per plant both at genotypic and phenotypic level. Path Coefficient Analysis revealed that, days to first pistillate flower anthesis (1.185) and number of fruits per plant (0.681) had maximum positive direct effect on fruit yield per plant.These are the real independent characters and have maximum contribution towards fruit yield per plant at genotypic level. Whereas at phenotypic level, fruit yield per plant was imposed by number of fruits per plant (0.664) only. From the overall result, the traits like days to first pistillate flower anthesis and numbers of fruits per plant can be utilize for breeding programme to increase fruit yield per plant in pumpkin.

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