Genotypic and phenotypic situation of antimicrobial drug resistance of Escherichia coli in water and manure between biogas and non-biogas swine farms in central Thailand

Dawangpa, Atchara; Lertwatcharasarakul, Preeda; Ramasoota, Pongrama; Boonsoongnern, Alongkot; Ratanavanichrojn, Nattavut; Sanguankiat, Arsooth; Phatthanakunanan, Sakuna; Tulayakul, Phitsanu

doi:10.1016/j.jenvman.2020.111659

Cited by 20 publications

(6 citation statements)

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“…), carbapenem resistance could become established in bacterial populations as has been the case with resistance against extended spectrum cephalosporin in E. coli from swine. Some studies on pigs already indicate that carbapenem resistance might be highly prevalent in certain countries such as Thailand and Ghana ( Pokhrel et al, 2018 ; Dawangpa et al, 2021 ; Larbi et al, 2021 ). Also, fecal carriage rate of greater than 1% in pigs from China and India should be of significant concern.…”

Section: Discussionmentioning

confidence: 99%

Global Distribution of Extended Spectrum Cephalosporin and Carbapenem Resistance and Associated Resistance Markers in Escherichia coli of Swine Origin – A Systematic Review and Meta-Analysis

et al. 2022

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Third generation cephalosporins and carbapenems are considered critically important antimicrobials in human medicine. Food animals such as swine can act as reservoirs of antimicrobial resistance (AMR) genes/bacteria resistant to these antimicrobial classes, and potential dissemination of AMR genes or resistant bacteria from pigs to humans is an ongoing public health threat. The objectives of this systematic review and meta-analysis were to: (1) estimate global proportion and animal-level prevalence of swine E. coli phenotypically resistant to third generation cephalosporins (3GCs) and carbapenems at a country level; and (2) measure abundances and global distribution of the genetic mechanisms that confer resistance to these antimicrobial classes in these E. coli isolates. Articles from four databases (CAB Abstracts, PubMed/MEDLINE, PubAg, and Web of Science) were screened to extract relevant data. Overall, proportion of E. coli resistant to 3GCs was lower in Australia, Europe, and North America compared to Asian countries. Globally, <5% of all E. coli were carbapenem-resistant. Fecal carriage rates (animal-level prevalence) were consistently manifold higher as compared to pooled proportion of resistance in E. coli isolates. blaCTX–M were the most common 3GC resistance genes globally, with the exception of North America where blaCMY were the predominant 3GC resistance genes. There was not a single dominant blaCTX–M gene subtype globally and several blaCTX–M subtypes were dominant depending on the continent. A wide variety of carbapenem-resistance genes (blaNDM–, VIM–, IMP–, OXA–48, andKPC–) were identified to be circulating in pig populations globally, albeit at very-low frequencies. However, great statistical heterogeneity and a critical lack of metadata hinders the true estimation of prevalence of phenotypic and genotypic resistance to these antimicrobials. Comparatively frequent occurrence of 3GC resistance and emergence of carbapenem resistance in certain countries underline the urgent need for improved AMR surveillance in swine production systems in these countries.

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Section: Discussionmentioning

confidence: 99%

Global Distribution of Extended Spectrum Cephalosporin and Carbapenem Resistance and Associated Resistance Markers in Escherichia coli of Swine Origin – A Systematic Review and Meta-Analysis

et al. 2022

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“…CHINET surveillance of bacterial resistance across China in 2017 showed that E. coli, Klebsiella, and other clinical pathogens were more than 60% resistant to ampicillin [12]. E. coli is the most common gramnegative bacterial pathogen, and the resistance of E. coli strains has gradually increased owing to the frequent or excessive use of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Escherichia coli resistance to ampicillin

Yang¹,

Chang²,

Zhang³

et al. 2023

Preprint

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The abuse and misuse of antibiotics in medical treatment and livestock production have led to the generation of bacterial resistance, especially Escherichia coliresistance to ampicillin, which has compromised clinical treatment. To further understand the specific characteristics of E. coli resistance to ampicillin, we explored the growth and resistance characteristics of bacteria evolving from sensitive to resistant bacteria, with the aim of discovering new phenomena and patterns. In the present study, ampicillin-resistant E. coli strains with minimal inhibitory concentration (MIC) values of 4-, 16-, and 64-fold were obtained through artificial passage. Results showed that the diameter of the colony inhibition circle decreased at the same concentration of ampicillin and "microcolonization"appeared as bacterial resistance increased. Regarding growth rate, ampicillin-resistant bacteria grew more slowly than susceptible bacteria. The MIC value of ampicillin-resistant bacteria for cefpirome and cefazolin, which are both β-lactam antibiotics, increased as drug resistance increased. In addition, the intracellular concentration of ampicillin and the permeability of the cell membrane decreased as drug resistance increased. This study explored the changing drug resistance characteristics of E. coliampicillin-resistant bacteria to varying degrees, and provides a foundation for future studies investigating the resistance mechanism and clinical treatment of E. coli ampicillin-resistant bacteria.

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“…WHO emphasizes the importance of antimicrobial classes (agents) that are used in humans being targeted for antimicrobial susceptibility surveillance in livestock, including aminoglycosides (gentamicin), carbapenems (meropenem), cephalosporins (3rd, 4th, and 5th generation: ceftriaxone, cefepime, ceftaroline, ceftobiprole), glycopeptides (vancomycin), amoxicillinclavulanic-acid, polymyxins (colistin), and quinolones (ciprofloxacin). Due to high prevalence and incidence of AMR rates in the pig industries in South-East Asian (ASEAN) countries, a regional database of AMR surveillance has been developed Nhung et al, 2016;Lugsomya et al, 2018a,b;Mobasseri et al, 2019;Vounba et al, 2019;Khine et al, 2020;World Health Organization [WHO], 2020;Dawangpa et al, 2021). It is important to emphasize that SEA is a diverse region, where different levels of development, customs, laws, and regulations among different countries in the region.…”

Section: Introductionmentioning

confidence: 99%

Reducing the Risk of Transmission of Critical Antimicrobial Resistance Determinants From Contaminated Pork Products to Humans in South-East Asia

et al. 2021

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Antimicrobial resistance (AMR) is a critical challenge worldwide as it impacts public health, especially via contamination in the food chain and in healthcare-associated infections. In relation to farming, the systems used, waste management on farms, and the production line process are all determinants reflecting the risk of AMR emergence and rate of contamination of foodstuffs. This review focuses on South East Asia (SEA), which contains diverse regions covering 11 countries, each having different levels of development, customs, laws, and regulations. Routinely, here as elsewhere antimicrobials are still used for three indications: therapy, prevention, and growth promotion, and these are the fundamental drivers of AMR development and persistence. The accuracy of detection of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) depends on the laboratory standards applicable in the various institutes and countries, and this affects the consistency of regional data. Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae are the standard proxy species used for indicating AMR-associated nosocomial infections and healthcare-associated infections. Pig feces and wastewater have been suspected as one of the hotspots for spread and circulation of ARB and ARG. As part of AMR surveillance in a One Health approach, clonal typing is used to identify bacterial clonal transmission from the production process to consumers and patients – although to date there have been few published definitive studies about this in SEA. Various alternatives to antibiotics are available to reduce antibiotic use on farms. Certain of these alternatives together with improved disease prevention methods are essential tools to reduce antimicrobial usage in swine farms and to support global policy. This review highlights evidence for potential transfer of resistant bacteria from food animals to humans, and awareness and understanding of AMR through a description of the occurrence of AMR in pig farm food chains under SEA management systems. The latter includes a description of standard pig farming practices, detection of AMR and clonal analysis of bacteria, and AMR in the food chain and associated environments. Finally, the possibility of using alternatives to antibiotics and improving policies for future strategies in combating AMR in a SEA context are outlined.

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Genotypic and phenotypic situation of antimicrobial drug resistance of Escherichia coli in water and manure between biogas and non-biogas swine farms in central Thailand

Cited by 20 publications

References 33 publications

Global Distribution of Extended Spectrum Cephalosporin and Carbapenem Resistance and Associated Resistance Markers in Escherichia coli of Swine Origin – A Systematic Review and Meta-Analysis

Global Distribution of Extended Spectrum Cephalosporin and Carbapenem Resistance and Associated Resistance Markers in Escherichia coli of Swine Origin – A Systematic Review and Meta-Analysis

Analysis of Escherichia coli resistance to ampicillin

Reducing the Risk of Transmission of Critical Antimicrobial Resistance Determinants From Contaminated Pork Products to Humans in South-East Asia

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