Genotypic diversity among<i>Bacillus licheniformis</i>strains from various sources

Clerck, Elke De; Vos, Paul De

doi:10.1016/s0378-1097(03)00935-2

Cited by 51 publications

(42 citation statements)

References 40 publications

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“…For B. pumilus, a lot of different patterns could be generated, indicating a high resolution of the fingerprinting technique for this species. For B. licheniformis, however, only two major types could be found, which could indicate a lower resolution of the fingerprinting method for this species even though this limited heterogeneity observed is in agreement with earlier studies (De Clerck & De Vos, 2004;Ishihara et al, 2002).…”

Section: Article In Presssupporting

confidence: 91%

“…Eight out of 176 isolates appeared separately in the cluster. Earlier studies also described the presence of two groups of B. licheniformis based on bacitracin synthetase gene sequence, erythromycin resistance, rep-PCR and rpoB and gyrA sequencing (De Clerck & De Vos, 2004;Ishihara, Takoh, Nishibayashi, & Sato, 2002 The B. pumilus dendrogram comprised the largest diversity of isolates. All but three strains isolated at 20 1C turned out to be true psychrotrophic strains.…”

Section: Article In Pressmentioning

confidence: 99%

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Intraspecific genotypic diversity of Bacillus species from raw milk

Jonghe¹,

Coorevits

Vandroemme

et al. 2008

International Dairy Journal

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Section: Article In Presssupporting

confidence: 91%

Section: Article In Pressmentioning

confidence: 99%

Intraspecific genotypic diversity of Bacillus species from raw milk

Jonghe¹,

Coorevits

Vandroemme

et al. 2008

International Dairy Journal

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“…Pathogenicity Testing for B. licheniformis R2 B. licheniformis has a generally regarded as safe (GRAS) status and it is exploited industrially for large-scale production of different bioproducts; recently, it is recognized as a human pathogen, and more and more questions arise concerning the safety aspects of exploiting this organism [16,17]. Also, various cases of B. licheniformis-associated clinical infections are reported mainly in immunocompromised hosts, so questions arises for accidental exposure to human contact while utilizing our B. licheniformis R2 strain for biosurfactant production at higher scale.…”

Section: Resultsmentioning

confidence: 99%

Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Joshi

Subramanian

Desai

2015

Appl Biochem Biotechnol

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The biosurfactant produced by Bacillus licheniformis R2 was characterized and studied for enhancing the heavy crude oil recovery at 80 °C in coreflood experiments. The strain was found to be nonpathogenic and produced biosurfactant, reducing the surface tension of medium from 70 to 28 mN/m with 1.1 g/l yield. The biosurfactant was quite stable during exposure to elevated temperatures (85 °C for 90 days), high salinity (10 % NaCl), and a wide range of pH (5-12) for 10 days. It was characterized as lipopeptide similar to lichenysin-A, with a critical micelle concentration of about 19.4 mg/l. The efficiency of crude biosurfactant for enhanced oil recovery by core flood studies revealed it to recovering additional 37.1 % oil from Berea sandstone cores at 80 °C. The results are indicative of the potential for the development of lipopeptide biosurfactant-based ex situ microbial enhanced heavy oil recovery from depleting oil fields with extreme temperatures.

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“…In addition to confirming FAME identifications, ARDRA enabled nine additional identifications at the species level. ARDRA clusters 4,6,7,8,24,28,29, and 30 and strain R-6443 were identified as Bacillus smithii, B. circulans, Bacillus oleronius, B. sporothermodurans, Brevibacillus brevis, Aneurinibacillus aneurinilyticus, Aneurinibacillus thermoaerophilus, Virgibacillus proomii and Paenibacillus thiaminolyticus, respectively ( Table 4). The strains in ARDRA clusters 6, 7, 8, 12, 13, and 24 grouped together with the type strain of the respective species analyzed simultaneously in this study.…”

Section: Resultsmentioning

confidence: 99%

Incidence and Diversity of Potentially Highly Heat-Resistant Spores Isolated at Dairy Farms

Scheldeman

Pil

Herman

et al. 2005

Appl Environ Microbiol

122

105

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The presence of highly heat-resistant spores of Bacillus sporothermodurans in ultrahigh-temperature or sterilized consumer milk has emerged as an important item in the dairy industry. Their presence is considered undesirable since they hamper the achievement of commercial sterility requirements. By using a selective 30-min heat treatment at 100°C, 17 Belgian dairy farms were screened to evaluate the presence, sources, and nature of potentially highly heat-resistant spores in raw milk. High numbers of these spores were detected in the filter cloth of the milking equipment and in green crop and fodder samples. About 700 strains were isolated after the selective heating, of which 635 could be screened by fatty acid methyl ester analysis. Representative strains were subjected to amplified ribosomal DNA restriction analysis, 16S rRNA gene sequencing, percent G؉C content, and DNA-DNA reassociations for further identification. The strain collection showed a remarkable diversity, with representatives of seven aerobic spore-forming genera. Bacillus licheniformis and Bacillus pallidus were the most predominant species overall. Twenty-three percent of the 603 spore-forming isolates proved to belong to 18 separate novel species. These findings suggest that the selective heating revealed a pool of unknown organisms with a higher heat-resistant character. This study showed that high spore counts can occur at the dairy farm and that feed and milking equipment can act as reservoirs or entry points for potentially highly heat-resistant spores into raw milk. Lowering this spore load by good hygienic measures could probably further reduce the contamination level of raw milk, in this way minimizing the aerobic spore-forming bacteria that could lead to spoilage of milk and dairy products. Assessment and characterization of this particular flora are of great importance to allow the dairy or food industry to adequately deal with newly arising microbiological problems.

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Genotypic diversity amongBacillus licheniformisstrains from various sources

Cited by 51 publications

References 40 publications

Intraspecific genotypic diversity of Bacillus species from raw milk

Intraspecific genotypic diversity of Bacillus species from raw milk

Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Incidence and Diversity of Potentially Highly Heat-Resistant Spores Isolated at Dairy Farms

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