Wheat is one of the earliest cultivated plants and second major cereal crop of the world that contains human food essentials. Due to climatic variations, crop is exposed to severe environmental conditions. Rising global temperature is expected to result in severe climate shifts in future. High temperature exerts negative effects on wheat phenology and grain production. The selection of heat-tolerant wheat germplasm is the best stress management strategy. This research study presents the fieldbased evaluation of 100 wheat genotypes under both normal and heat stress conditions. The data of twelve phenotypic traits were recorded under control and heat stress trials. There were diverse variations observed among genotypes for morphological characters. The high yielding selected wheat lines 68. 111-56, CHEN-84, CHEN-74, IWA8611400-65, CHEN-95, CHEN-96, CHEN-86, CHEN-80, 68.111-60 and CHEN-92 were subjected to DNA genotyped with Simple Sequence Repeat marker(SSR) viz; WMC 18, CFA2129, WMC 367, CFD 48, WMC44 and WMC 798. The genetic diversity ranged from 0 to 0.25 with an average value of 0.06. Unweighted pair group of arithmetic means cluster analysis grouped these lines into clusters. Most of the genetic diversity was observed for CHEN-74 and CHEN-96 in cluster. The study has identified genetically diverse, heat tolerant and high yielding wheat genotypes with possible exploitation in development of climate-smart wheat varieties.