Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle

Franco, Marília Masello Junqueira; Ribeiro, Márcio Garcia; Pavan, Fernando Rogério; Miyata, Masanobu; Heinemann, Marcos Bryan; Filho, Antônio Francisco de Souza; Cardoso, Rosilene Fressatti; Almeida, Aryadne Larissa de; Sakate, Ricardo Ichiro; Paes, Antônio Carlos

doi:10.1016/j.tube.2017.02.006

Cited by 21 publications

(37 citation statements)

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“…Only one lymph node showed the simultaneous isolation of R. equi and M. bovis (data not shown). This result is not surprising as mycobacteria are recognized as a major agent of tuberculoid gross changes in cattle (Dvorska et al., ; Sahraoui et al., ), including in Brazil (Franco et al., ). Nevertheless, a high prevalence (31.0%) of R. equi recovered from lymphadenitis was found in cattle in the current study, indicating that this microorganism should be considered itself an agent of lymphadenitis in this species (Dvorska et al., ; Flynn et al., ; Soedarmanto et al., ), and an important pathogen to the differential diagnosis of mycobacteriosis, as lesions caused by those bacteria are macroscopically indistinguishable (Soedarmanto et al., ).…”

Section: Resultsmentioning

confidence: 91%

Novel bovine-associated pVAPN plasmid type in Rhodococcus equi identified from lymph nodes of slaughtered cattle and lungs of people living with HIV/AIDS

Ribeiro

Lara

Silva

et al. 2017

Transbound Emerg Dis

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Rhodococcus equi is a well-recognized Gram-positive intracellular facultative bacterium that is opportunistic in nature, which causes pyogranulomatous infections in humans and multiple host animals. The pathogenicity of the microorganism has been attributed to the presence of plasmid-encoded virulence-associated proteins (Vap). To date, three host-associated virulence plasmid types of R. equi have been identified as follows: the circular pVAPA and pVAPB, related, respectively, to equine and porcine isolates, and a recently described linear pVAPN plasmid associated with bovine strains, although these three types are found in human isolates. Recent phylogenomic studies support the evidence that human R. equi infection is zoonotically acquired. Nevertheless, data regarding distribution and prevalence of the host-adapted virulence plasmid types of R. equi isolated from meat animals are scarce or unnoticed. Here, the three host-associated virulence plasmid types (pVAPA, pVAPB, and pVAPN) were investigated in 154 R. equi isolates recovered from lymph nodes of cattle with lymphadenitis (n = 31), faeces of cattle without enteric signs (n = 49), as well as different clinical specimens from human patients (n = 74). The analysis of virulence profile of 74 R. equi from humans revealed six (8.1%) isolates pVAPB (type 8), two (2.7%) pVAPN, and one (1.3%) pVAPB (type 11), all of which were from lung samples from people living with HIV/AIDS. From the lymph node samples of cattle, 41.9% (13 of 31) isolates revealed pVAPN type, whereas all isolates from faecal samples were negative for three host-associated types. Here, recently described bovine-associated pVAPN type was detected in R. equi isolates recovered from the lungs of people living with HIV/AIDS and lymph nodes from slaughtered cattle intended for human consumption; a finding that represents a public health concern, mainly in countries where undercooked or raw meat are traditionally consumed.

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Section: Resultsmentioning

confidence: 91%

Novel bovine-associated pVAPN plasmid type in Rhodococcus equi identified from lymph nodes of slaughtered cattle and lungs of people living with HIV/AIDS

Ribeiro

Lara

Silva

et al. 2017

Transbound Emerg Dis

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“…Multidrug-resistant M. bovis outbreaks in humans have occurred, some with serious consequences [ 16 , 18 , 47 ]. M. bovis isolates from cattle with drug resistance towards either RIF or INH or towards both drugs have already been documented [ 23 , 29 ]. Drug susceptibility surveillance of M. bovis from animals may therefore contribute to preventing the transmission of multidrug-resistant strains to humans and to controlling possible outbreaks.…”

Section: Discussionmentioning

confidence: 99%

“…While mycobacteria isolated from human cases are generally assessed for drug susceptibility, studies on antitubercular drug susceptibility testing of M. bovis and M. caprae isolated from animals are limited [ 23 – 29 ]. So far, M. bovis isolates from cattle resistant to INH and rifampin (RIF) have been documented in Italy [ 23 ] and Brazil [ 29 ], to the best of our knowledge. Monitoring of antitubercular drug resistance of M. bovis isolated from animals may thus contribute to reducing the risk of drug-resistant M. bovis transmission from animals to humans and among human beings.…”

Section: Introductionmentioning

confidence: 99%

Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle

et al. 2018

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BackgroundA rising isolation trend of drug-resistant M. bovis from human clinical cases is documented in the literature. Here we assessed Mycobacterium tuberculosis complex isolates from cattle for drug susceptibility by the gold standard agar proportion method and a simplified resazurin microtitre assay (d-REMA). A total of 38 M. tuberculosis complex strains, including M. bovis (n = 36) and M. caprae (n = 2) isolates, from cattle in Tunisia were tested against isoniazid, rifampin, streptomycin, ethambutol, kanamycin and pyrazinamide.ResultsM. caprae isolates were found to be susceptible to all test drugs. All M. bovis strains were resistant to pyrazinamide, as expected. In addition, one M. bovis isolate showed high-level resistance to streptomycin (MIC > 500.0 μg/ml). Concordant results with the two methods were found. The most common target genes associated with streptomycin resistance, namely the rrs, rpsL and gidB genes, were DNA sequenced. A non-synonymous mutation at codon 43 (K43R) was found in the rpsL gene. To the best of our knowledge, this is the first report describing the isolation of a streptomycin-resistant M. bovis isolate from animal origin.ConclusionsAntitubercular drug susceptibility testing of M. bovis isolates from animals should be performed in settings where bTB is endemic in order to estimate the magnitude of the risk of drug-resistant tuberculosis transmission to humans.

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“…It is important to consider the occurrence of MDR M.bovis because many studies provided evidence that this pathogen has been isolated from animals. A recent study published in 2017 reported that Mycobacterium bovis resistant to both rifampicin and isoniazid in a study conducted in Brazil [42].…”

Section: Figure 1: Stages For Eligibility Criteria and Study Selectiomentioning

confidence: 99%

Prevalence of Multidrug Resistance Mycobacterium Bovis in Human: A Systematic Review and Meta-Analysis

2019

JHMN

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Introduction:Human infection with multidrug resistant mycobacterium bovis (MDR-M.bovis) is very rare. Recently, infection with this type of bacteria has been described. The WHO strategy to end TB by 2035, recall for more efforts in the diagnosis and treatment of mycobacterium bovis particularly in resource limited countries. The aim of this systematic review was to determine the prevalence of multidrug resistant tuberculosis M.bovis infects human. Methods:International database including Medline, EMBASE, and Web of Sciences, Scopus, and ScienceDirect were searched for related literature. Google Scholar searching engines as well as references list were explored. Studies met the inclusion criteria were included in the systematic review. Random effects model was used to estimate the pooled prevalence. Results:A total number of (4275) studies were retrieved and nine studies were included in the meta analysis. The overall estimated prevalence of the multidrug resistant Mycobacterium bovis was 0.04 (95% CI: 0.02, 0.06). Based one study quality, time and area of study, subgroup analysis were conducted to check for heterogeneity. The prevalence of MDR-M bovis in low quality studies was 0.04 (95% CI: 0.00, 0.06), and in high quality studies was 0.05 (95% CI: 0.02, 0.08). Conclusions:The finding of this study indicated that the prevalence of multidrug resistant M.bovis in human is low. However, more attention is needed in the diagnosis and treatment of M.bovis infection in Human.

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Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle

Cited by 21 publications

References 43 publications

Novel bovine-associated pVAPN plasmid type in Rhodococcus equi identified from lymph nodes of slaughtered cattle and lungs of people living with HIV/AIDS

Novel bovine-associated pVAPN plasmid type in Rhodococcus equi identified from lymph nodes of slaughtered cattle and lungs of people living with HIV/AIDS

Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle

Prevalence of Multidrug Resistance Mycobacterium Bovis in Human: A Systematic Review and Meta-Analysis

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