Genotyping by Random Amplified Polymorphic DNA Assay of Acinetobacter baumannii Isolated from Blood Culture of Patients with Hematological Malignancies

Khrulnova, S. A.; Fedorova, A.V.; Frolova, I.N.; Клясова, Г А

doi:10.31631/2073-3046-2020-19-4-38-47

Epidemiology and Vaccinal Prevention

2020

DOI: 10.31631/2073-3046-2020-19-4-38-47

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Genotyping by Random Amplified Polymorphic DNA Assay of Acinetobacter baumannii Isolated from Blood Culture of Patients with Hematological Malignancies

S. A. Khrulnova¹,

A.V. Fedorova²,

I.N. Frolova³

et al.

Abstract: Relevance. Acinetobacter baumannii is a significant nosocomial pathogen that can cause severe infections, especially in immunocompromised patients. Aims. This study aimed to investigate clonal diversity of A. baumannii isolated from blood culture in hematological patients by random amplified polymorphic DNA assay (RAPD). Materials & Methods. Genotyping of A.baumannii isolated from blood culture in hematological patients in 7 Russian hospitals (2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013)(2… Show more

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2022

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Cited by 2 publications

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Comparative Analysis of Bacterial Microflora Isolated from Adult Pneumonia Inpatients and Hospital Objects in the Amur Region

Bondarenko¹,

Курганова²,

Троценко³

et al. 2022

PH&LE

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Background: The COVID-19 pandemic has exacerbated the problem of healthcare-associated infections and worsened the situation with the hospital spread of antibiotic resistant strains potentially inducing nosocomial infections. Objective: To compare bacterial microflora isolated from adult pneumonia inpatients and hospital objects and to assess the risk of hospital-acquired infections in the Amur Region. Materials and methods: The study was conducted in a multidisciplinary and a “COVID” hospital hereinafter referred to as Hospitals 1 and 2, respectively. Parallel bacteria culture testing of 519 sputum samples from 284 inpatients and 486 wipe samples collected weekly during 12 weeks in both hospitals was carried out using a classical method. AutoSCAN-4 and Vitek® 2 Compact 30 analyzers were applied to identify pathogens and determine their antimicrobial susceptibility. Results: Along with the detection of pathogenic bacteria in 33.8 % of hospitalized patients with pneumonia, we found pathogens in 23.3 % and 12.4 % of wipe samples from Hospitals 1 and 2, respectively, and established that the proportion of Gram-negative bacteria in them was 44.9 % and 15.4 % while the share of multidrug-resistant strains was 32.7 % and 3.8 %. The significant differences observed can be attributed to a more stringent disinfection regime in Hospital 2. The structure of multidrug-resistant Gram-negative bacteria was noted for a high proportion of carbapenem-resistant organisms isolated from both sputum (20.0 %) and wipe (38.9 %) samples. Conclusion: Our monitoring results helped define the composition and ratio of bacterial pathogens isolated from pneumonia cases and hospital objects. A wide range of pathogens, including those resistant to antibiotics, often detected in wipe samples and their accumulation over time indicate the potential hazard of the hospital environment posing an increased risk of hospital-acquired infections.

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Comparative Analysis of Bacterial Microflora Isolated from Adult Pneumonia Inpatients and Hospital Objects in the Amur Region

Bondarenko¹,

Курганова²,

Троценко³

et al. 2022

PH&LE

View full text Add to dashboard Cite

show abstract

The using of the polymerase chain reaction for the detection of resistance genes in gram-negative bacteria in routine practice in a pediatric hospital

et al. 2022

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Objective - assessment of RT-PCR for the detection of carbapenem-resistance genes in gram-negative bacteria. A total, 499 strains of gram-negative microorganisms isolated in two pediatric hospitals in 2019-2020 were studied. Species identification was performed using MALDI-ToF mass-spectrometry (Bruker Daltonics, Germany). Meropenem and imipenem minimal inhibitory concentration (MIC) was determined by E-test method (BioMerieux, France). The presence of acquired carbapenemase genes of IMP, NDM, VIM, KPC, OXA-48, OXA-23, OXA-40, OXA-58-groups was determined by RT-PCR. Klebsiella pneumoniae (34%), Escherichia coli (4%), Serratia marcescens (6%) and other members of Enterobacterales (6%), also gram-negative non-glucose-fermenting bacteria Acinetobacter baumannii (14%), Pseudomonas aeruginosa (36%) were found among selected strains. Carbapenemase production was found in 385 isolates (77%). The main mechanism determining carbapenem resistance in P. aeruginosa was the production of blaVIM (100%). A. baumanii strains harbored OXA-23 (55%) and OXA-40 (45%) carbapenemases. The major determinant of carbapenem resistance in K. pneumoniae isolates was OXA-48 carbapenemase, detected in 63% strains, 13% of the strains possessed blaNDM-group, 16% isolates had a combination of blaNDM-group and blaOXA-48-like. Carbapenemase of KPC-group was found in 8% K. pneumoniae strains. OXA-48 carbapenemase prevailed (95%) among S. marcescens strains. Most of E. coli isolates harbored metallo-beta-lactamase NDM (89%). Other members of Enterobacterales most often had OXA-48 carbapenemase (57%), 39% of the isolates carried blaNDM-group. In one strain, a combination of blaNDM-group and blaOXA-48-like was discovered. RT-PCR is a fast and reliable method for the detection of acquired carbapenemases and can be recommended for routine use in bacteriological laboratories.

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Genotyping by Random Amplified Polymorphic DNA Assay of Acinetobacter baumannii Isolated from Blood Culture of Patients with Hematological Malignancies

Cited by 2 publications

References 18 publications

Comparative Analysis of Bacterial Microflora Isolated from Adult Pneumonia Inpatients and Hospital Objects in the Amur Region

Comparative Analysis of Bacterial Microflora Isolated from Adult Pneumonia Inpatients and Hospital Objects in the Amur Region

The using of the polymerase chain reaction for the detection of resistance genes in gram-negative bacteria in routine practice in a pediatric hospital

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