2017
DOI: 10.1177/0300060516677155
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Genotyping Leishmania promastigotes isolated from patients with cutaneous leishmaniasis in south-eastern Turkey

Abstract: ObjectiveCutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods.MethodsNovy–MacNeal–Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, … Show more

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Cited by 12 publications
(12 citation statements)
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“…The presence of L. major in southern Turkey is due to the arrival of Syrian refugees since 2011 to these areas where L. major has not been identified previously (83). There is high L. tropica heterogeneity in Turkey with distinct clades (84)(85)(86). There are genetic similarities between northwest Iranian L. infantum strains and hybrid strains of L. infantum/L.…”
Section: Distribution Of CL and Related Causative Agents In The Neighmentioning
confidence: 99%
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“…The presence of L. major in southern Turkey is due to the arrival of Syrian refugees since 2011 to these areas where L. major has not been identified previously (83). There is high L. tropica heterogeneity in Turkey with distinct clades (84)(85)(86). There are genetic similarities between northwest Iranian L. infantum strains and hybrid strains of L. infantum/L.…”
Section: Distribution Of CL and Related Causative Agents In The Neighmentioning
confidence: 99%
“…donovani in Cukurova in southeast Turkey (17). The recent arrival of refugees from Syria will add additional genetic heterogeneity to the L. tropica genetic pool in Turkey (86). Aside from humans, L. tropica, L. major, and L. infantum have been isolated frequently in cats in Turkey and may be influenced by different ecological cycles (87,88).…”
Section: Distribution Of CL and Related Causative Agents In The Neighmentioning
confidence: 99%
“…According to (Eksi et al, 2017) with some modification in the method of collecting the aspiration fluid, the lesion sites were cleaned with 70% alcohol. there my a syringe (1ml, 30G*1/2) when intradermal pentostam injection at the periphery was done, we were aspirated the fluids and the blood that oozing from the sites by capillary tubes with anti-coagulants and prepared slide to examine directly by microscopy after staining and the other was saved in screw cap container with the Sterile NaCl solution as dilution fluid and incubated in refrigerator (4 0 c) until DNA extraction.…”
Section: Methodsmentioning
confidence: 99%
“…and preparation with no amastigotes is considered negative (-ve) for Leishmania spp. All results were recorded (Rahi, 2015;Eksi et al, 2017).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation