Genotyping of Brucella melitensis strains from dromedary camels (Camelus dromedarius) from the United Arab Emirates with multiple-locus variable-number tandem repeat analysis

Gyuranecz, Miklós; Wernery, U.; Kreizinger, Zsuzsa; Juhász, Judit; Felde, Orsolya; Nagy, P.

doi:10.1016/j.vetmic.2016.02.009

Cited by 17 publications

(18 citation statements)

References 11 publications

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“…Our newly developed MLVA-13Bc method was specifically designed to discriminate strains from within the species of B . canis , and had higher discriminatory power than previous assays 25, 31, 32 . Although our data is not associated with pathogenesis as such information is not available, the new MLVA-13Bc method genotyped each of the 229 isolates in our collection, indicating that it is a useful molecular epidemiological tool to trace the source of strain introduction in an event of a B .…”

Section: Discussionmentioning

confidence: 61%

Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Yang

Wang

Poulsen

et al. 2017

Sci Rep

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Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B. canis isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 B. canis isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a B. canis outbreak.

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Section: Discussionmentioning

confidence: 61%

Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Yang

Wang

Poulsen

et al. 2017

Sci Rep

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“…Both the groups appeared as a branch in the East Mediterranean clade tree ( Fig 5 ). Interestingly, the genotype 64 showed a minimum distance of 1.0 with MLVA-8 type-43 of a strain isolated in the United Arab Emirates, a country in the East Mediterranean Region [ 41 ]. Amongst the new MLVA-8 types, five strains (belonging to K1, K2, K4 and K8) clustered with the East Mediterranean region, two strains (belonging to K5) clustered with the African clade and one strain (belonging to K6) formed a branch in the West Mediterranean Region ( Fig 5 ).…”

Section: Resultsmentioning

confidence: 99%

“…Similar results have been reported by other investigators [ 28 , 40 , 57 , 58 ]. A comparison with strains from other regions revealed considerable variation in the VNTRs associated with the same alleles [ 27 , 29 , 41 ]. Even the strains from same clade exhibit allele differences, except for Bruce 45, which represents a single allele in majority of cases [ 27 , 29 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Kilic and co-workers reported that the strains isolated in Turkey were mostly from the East Mediterranean region and not from other parts of the world [ 29 ]. Interestingly, the genotype 64 was only at a distance of 1.0 with the genotype 43, which is commonly found in Turkey [ 29 ] and UAE [ 41 ]. Genotype 45 was also found previously in Turkey [ 39 ] and China [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…In 2006, a highly discriminatory method for genotyping Brucella , based on multilocus variable-number tandem-repeat analysis (MLVA), was developed by Le Fleche and co-workers [ 39 ]. This method has been used to type various species and strains of Brucella with fine-scale resolution of closely related isolates [ 27 – 34 , 40 , 41 ]. The MLVA-16 system comprises of eight minisatellite or Panel 1 markers (Bruce06, Bruce08, Bruce11, Bruce12, Bruce42, Bruce43, Bruce45, and Bruce55) and eight complementary microsatellite or Panel 2 markers divided into panel 2A (Bruce18, Bruce19, and Bruce21) and panel 2B (Bruce04, Bruce07, Bruce09, Bruce16, and Bruce30).…”

Section: Introductionmentioning

confidence: 99%

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Species identification and molecular typing of human Brucella isolates from Kuwait

et al. 2017

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Brucellosis is a zoonotic disease of major concern in Kuwait and the Middle East. Human brucellosis can be caused by several Brucella species with varying degree of pathogenesis, and relapses are common after apparently successful therapy. The classical biochemical methods for identification of Brucella are time-consuming, cumbersome, and provide information limited to the species level only. In contrast, molecular methods are rapid and provide differentiation at intra-species level. In this study, four molecular methods [16S rRNA gene sequencing, real-time PCR, enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus variable-number tandem-repeat analysis (MLVA)-8, MLVA-11 and MLVA-16 were evaluated for the identification and typing of 75 strains of Brucella isolated in Kuwait. 16S rRNA gene sequencing of all isolates showed 90–99% sequence identity with B. melitensis and real-time PCR with genus- and species- specific primers identified all isolates as B. melitensis. The results of ERIC-PCR suggested the existence of 75 ERIC genotypes of B. melitensis with a discriminatory index of 0.997. Cluster classification of these genotypes divided them into two clusters, A and B, diverging at ~25%. The maximum number of genotypes (n = 51) were found in cluster B5. MLVA-8 analysis identified all isolates as B. melitensis, and MLVA-8, MLVA-11 and MLVA-16 typing divided the isolates into 10, 32 and 71 MLVA types, respectively. Furthermore, the combined minimum spanning tree analysis demonstrated that, compared to MLVA types discovered all over the world, the Kuwaiti isolates were a distinct group of MLVA-11 and MLVA-16 types in the East Mediterranean Region.

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Brucella

Ashford

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2022

Molecular Typing in Bacterial Infections, Volume II

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Genotyping of Brucella melitensis strains from dromedary camels (Camelus dromedarius) from the United Arab Emirates with multiple-locus variable-number tandem repeat analysis

Cited by 17 publications

References 11 publications

Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Species identification and molecular typing of human Brucella isolates from Kuwait

Brucella

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