Genotyping of four loci in Hungarian Yellow and broiler chickens

Abstract

Traditional selection has led to remarkable differences in allele frequencies among various chicken breeds. Indigenous and broiler-type chicken populations were genotyped for polymorphisms in thyroid hormone responsive Spot14α, prolactin (PRL), IGF-binding protein 2 (IGFBP2), and somatostatin (SST) genes in order to determine potential utilisation type-associated allele frequencies. Significant (P < 0.05) differences were detected between Hungarian Yellow and broiler populations for Spot14α, PRL, and IGFBP2 allele frequencies, whereas the same SST allele (A) was fixed in both groups. In this study, the most significant associations (P < 0.05) were found between the IGFBP2 genotypes and the measured traits (body weight, carcass weight, breast muscle weight with or without skin, breast muscle weight as a percentage of carcass weight) in the broiler population. The results can be applied for the evaluation of polymorphism effects in the analysed populations; however, contradictory allele effects in different breeds and hybrids indicate the need for cautious marker utilisation in selection programmes.

Sex-Specific Control of Muscle Mass: Elevated IGFBP Proteolysis and Reductions of IGF-1 Levels Are Associated with Substantial Loss of Carcass Weight in Male DU6PxIGFBP-2 Transgenic Mice

“…If the loss of muscle mass in human subjects is monitored, the proteolysis of IGFBPs can also represent a novel content of biomarker information. Our results may have implications for IGFBP-biomarker research in vertebrates [ 6 , 7 , 8 , 9 , 10 , 11 ] since circulating concentrations of IGFBP-2 or other IGFBPs may not as a rule be interpreted in only one direction and clearly have a sex-related physiological background.…”

“…Accordingly, in Angus bulls and in conjunction with parameters of growth hormone secretion, concentrations of circulating intact IGFBP-2 were informative for the prediction of average daily weight gain [ 6 ]. On the genomic level, studies in pigs [ 7 , 8 ] and chicken [ 9 , 10 , 11 ] have identified single nucleotide polymorphisms of the gene encoding IGFBP-2 associated with altered growth and carcass weight, and the inclusion of the IGFBP-2 locus in selection programs has been discussed. The negative effect of IGFBP-2 on somatic growth seems evolutionary conserved and was provided in zebrafish characterized by a duplicated IGFBP-2 gene [ 12 ].…”