Genotyping of helianthus based on microsatellite sequences

Abstract

Analysis of microsatellite sequences was used for estimation of genetic diversity of cultivated and wild sunflower and usefulness for genotype identification, genetic purity and "hybridity range" definition. In total 13 SSRs were tested. Unique alleles for some wild sunflower species were found. Eight out of 13 SSR loci were polymorphic for the investigated inbred lines. Four of the investigated microsatellites are suitable and sufficient for genome identification and determination of parentage of hybrids. A procedure is proposed for sunflower genotype registration based on DNA-typing.

Characterization of Bulgarian Sunflower Cultivars and Inbred Lines with Microsatellite Markers

“…Among molecular markers, microsatellites (Simple Sequence Repeats -SSRs) are the most reliable markers for cultivars identifi cation, genetic diversity evaluation and property rights protection. Due to their high polymorphism, random distribution and co-dominant Mendelian inheritance, microsattelite markers were widely applied in sunfl ower researches for identifi cation of inbred lines, cultivars, wild species (3,4,5,6,9,15,19,20,22,25), construction of genetic maps (23) and identifi cation of QTLs for important agronomic traits (10,12,16,17).…”

Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing

“…ORS primers, being codominant, are generally used for sunflower genotyping in order to determine the genetic diversity between wild and cultivated sunflower, or sunflower lines and hybrids (Solodenko and Sivolap, 2005;Hvarleva et al, 2007). In this study we used thirteen SSR primers, created by Tang et al (2002) for testing sunflower isogenic lines in final stages of selection, in order to determine the degree of similarity between the parental lines and their F 6 and F 7 progeny lines.…”

“…Because of their multiallelic and highly polymorphic nature, microsatellite markers or SSRs are currently becoming the preferred technique for the molecular breeding in plant species. In sunflower (Helianthus annuus L.), microsatellites have been particularly useful in the studies of phylogenic relationships, genotype identification and calculation of genetic relationship between inbred lines (Smith et al, 2000;Paniego et al, 2002;Solodenko et al, 2005;Antonova et al, 2006;Hvarleva et al, 2007).…”

Microsatellite Marker Application in Sunflower (Helianthus AnnuusL.) Fingerprinting

“…Bulked DNA samples were used for further analysis. Primers were chosen on the basis of scolarly publications and the Sunflower CMap Database (http://sunflower.uga.edu/ cmap/), by the level of polymorphism and their successful use for sunflower genotyping (23).…”