Genotyping of Human Pappilomavirus in Cervical Precancerous Lesion and Squamous Cell Carcinoma at Dr. Soetomo Hospital, Surabaya, Indonesia

Mastutik, Gondo; Rahmi, Alia; Rahniayu, Alphania; Rahaju, Anny Seio; Kurniasari, Nila; Putra, Suhartono Taat

doi:10.21010/ajid.v12i1s.2

Cited by 10 publications

(13 citation statements)

References 8 publications

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“…We found that 59% of the HPV-positive samples had multiple HR-HPV infections. Other studies reported multiple HPV infections: 68.2% in HSIL [35] and 13.2% in FFPE samples [36]. Another study reported that 38.2% of the cases had a multiple HPV infections, which is lower than the value reported in our study (59%).…”

Section: Discussioncontrasting

confidence: 93%

Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Oyervides-Muñoz

Pérez-Maya

Sánchez‐Domínguez

et al. 2020

Viruses

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Persistent high-risk human papillomavirus (HR-HPV) infections play a major role in the development of invasive cervical cancer (CC), and screening for such infections is in many countries the primary method of detecting and preventing CC. HPV typing can be used for triage and risk stratification of women with atypical squamous cells of undetermined significance (ASC-US)/low-grade cervical lesions (LSIL), though the current clinical practice in Mexico is to diagnose CC or its preceding conditions mainly via histology and HR-HPV detection. Additional information regarding these HPV infections, such as viral load and co-infecting agents, might also be useful for diagnosing, predicting, and evaluating the possible consequences of the infection and of its prevention by vaccination. The goal of this follow-up hospital case study was to determine if HPV types, multiple HPV infections, and viral loads were associated with infection persistence and the cervical lesion grade. A total of 294 cervical cytology samples drawn from patients with gynecological alterations were used in this study. HPV types were identified by real-time PCR DNA analysis. A subset of HPV-positive patients was reevaluated to identify persistent infections. We identified HPV types 16, 18, and 39 as the most prevalent. One hundred five of the patients (59%) were infected with more than one type of HPV. The types of HPV associated with multiple HPV infections were 16, 18, and 39. In the follow-up samples, 38% of patients had not cleared the initially detected HPV infection, and these were considered persistent. We found here an association between multiple HPV infections and high viral loads with and infection persistence. Our findings suggest there are benefits in ascertaining viral load and multiple HPV infections status of HR-HPV infections for predicting the risk of persistence, a requirement for developing CC. These findings contribute to our understanding of HPV epidemiology and may allow screening programs to better assess the cancer-developing risks associated with individual HR-HPV infections.

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Section: Discussioncontrasting

confidence: 93%

Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Oyervides-Muñoz

Pérez-Maya

Sánchez‐Domínguez

et al. 2020

Viruses

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“…► The introduction of the HPV bivalent vaccine as part of the national vaccination program is recommended. The inconclusive results of a single genotype were further analyzed with AMPLIQUALITY HPV-TYPE EXPRESS (version 3.0, AB ANALITICA, Italy) according to the manufacturer's instructions (18). This kit can identify 40 HPV individual genotypes based on the amplification of a 145-bp fragment in the L1 region of the HPV genome, followed by a reverse line blot assay.…”

Section: Hpv Detection Through Conventional Polymerase Chain Reactionmentioning

confidence: 99%

Prevalence and Genotype Distribution of Human Papillomavirus Among a Subpopulation of Jordanian Women

Khasawneh¹,

Asali²,

Kilani³

et al. 2020

International Journal of Women’s Health and Reproduction Scienc

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Objectives: Human papillomavirus (HPV) infection is the leading sexually-transmitted infection (STI) worldwide and the main etiology of cervical cancer. HPV infection rates are important in directing vaccination policies and screening for cervical cancer. Unfortunately, no recent reports have evaluated the prevalence of this infection among Jordanian women despite major globalization-driven changes in sexual behavior. Accordingly, this study aimed to determine the prevalence of HPV infection and its genotypic distribution in the cervical samples of Jordanian women. Materials and Methods: The study was carried out at Prince Hamza Hospital (Amman, Jordan) during 2016-2017. Women (15-75 years old) were randomly selected for cervical cell collection. DNA was extracted and then amplified using MY09/11 and GP5+/6+ consensus primers. Finally, positive samples were genotyped by applying real-time-polymerase chain reaction and reverse line blotting. Results: Fourteen out of 348 women tested positive for HPV with a prevalence rate of 4%. In addition, multiple HPV genotypes were observed in 36% (5/14) of infected women while single HPV genotype infection was found in 64% (9/14) of infected women. Further, high-risk (HR), potential high-risk (pHR), and low-risk (LR) HPV genotypes were detected in most cases with a 78.6% (11/14) infection rate, 42.9% (6/14), and 7.1% (1/14) of infected women, respectively. Eventually, 10 different genotypes were detected in infected women and HPV 16 was the most common type (42.9%, 6/14). Conclusions: Our data suggest that the prevalence of HPV infection among Jordanian women is below the global and regional rates. It is hoped that these data should facilitate the implementation of appropriate cervical cancer screening and future HPV vaccination programs.

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“…24 The common HR-HPV in men with condyloma acuminata was HPV 16 and followed by HPV 18.25 Our previous study showed that the most common HPV infected in men with condyloma acuminata was LR-HPV were HPV 6, HPV 11 and HR-HPV were HPV 18, 51, 52, 82 26 and in cervical precancerous lesion and squamous cell carcinoma were is HPV 16 (62.68%), then followed by HPV 18 (20.9%), HPV 45 (5.97%), 52 (5.97%), and 67 (4.48%). 27 However, condyloma acuminata is usually caused by LR-HPV infection, this finding demonstrated that LR-HPV co-infection with HR-HPV. HR-HPV such as HPV 16, HPV 18, HPV 45, HPV 52, and 67 was HPV have been founded in cervical cancer and precancerous lesion.…”

Section: Discussionmentioning

confidence: 88%

“…HR-HPV such as HPV 16, HPV 18, HPV 45, HPV 52, and 67 was HPV have been founded in cervical cancer and precancerous lesion. 27 Several other studies have also shown that HPV 16, as well as LR-HPV (HPV 6 and 11) were also found in penile intraepithelial neoplasia.8 HPV 16 and HPV 18 were founded on vulval and vaginal cancel cell.28 Persistent infection of HR-HPV is a risk factor for progression of cell transformation into malignant cells. The determination of genotype HPV, including HR-HPV can be used to predict the malignant transformation of condyloma acuminata into invasive cancer in the anogenital area and also to establish a preventive program by vaccination.…”

Section: Discussionmentioning

confidence: 98%

Distribution Genotype High Risk (HR) And Low Risk (LR) Human Papillomavirus (HPV) at Condyloma Acuminata

Mastutik

Rahniayu

Murtiastutik

et al. 2021

BHSJ

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Introduction: Condyloma acuminata that is also known as genital warts are one of the most common sexually transmitted that caused by infection of Human papillomavirus (HPV). Persistent infection of Low Risk (LR) or High risk (HR) HPV is a risk factor for progress into benign or malignant cancer. The objective is to analyze distribution of genotype LR-HPV and HR-HPV at condyloma acuminata in anogenital region. Methods: A cross sectional study using were 36 lesions from men and women of condyloma acuminata patients. All subject signed the informed consent and ethic obtained from our institution, number 382/Panke.KKE/V/2016. The specimen was used to histopathological examination and to identified 40 genotypes of HPV using a reverse line blot assay.Results: The All patients were diagnosed as condyloma acuminata, some with focus dysplasia and koilocytosis. All patients were positive for HPV, including LR-HPV were HPV 6, 11, 42, 54, 61, 81,87,89 and HR-HPV were HPV 18, 26, 45, 51, 52, 66, 67, 68B, 69, 82. The single infection of LR-HPV was 44.4%, multiple infection LR/LR-HPV was 13,9% and the multiple infection of LR/HR-HPV was 41.7%. The LR-HPV infected 70,6% and HR-HPV infected 29,4%. Conclusion: LR-HPV is the major infection of condyloma acuminata, in single infection or multiple infection with HR-HPV. The most common infections were HPV 11, followed by HPV 6, HPV 18, HPV 51, and HPV 82. The determination of genotype of HPV can be used to predict the malignant transformation.

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Genotyping of Human Pappilomavirus in Cervical Precancerous Lesion and Squamous Cell Carcinoma at Dr. Soetomo Hospital, Surabaya, Indonesia

Cited by 10 publications

References 8 publications

Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Multiple HPV Infections and Viral Load Association in Persistent Cervical Lesions in Mexican Women

Prevalence and Genotype Distribution of Human Papillomavirus Among a Subpopulation of Jordanian Women

Distribution Genotype High Risk (HR) And Low Risk (LR) Human Papillomavirus (HPV) at Condyloma Acuminata

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