Genotyping ofCampylobacter coliisolated from humans and retail meats using multilocus sequence typing and pulsed-field gel electrophoresis

Abstract

Aims: To determine the antimicrobial resistant profiles and clonality of Campylobacter coli isolated from clinically ill humans and retail meats. Methods and Results: A total of 98 C. coli isolates (20 from humans and 78 from retail meats) were phenotypically characterized. Antimicrobial susceptibility testing was done using agar dilution method for ciprofloxacin, gentamicin, erythromycin and doxycycline. Seventy C. coli isolates including humans (n = 20) and retail meats (n = 50) were genotyped by multilocus sequence typing (MLST) and pulsed‐field gel electrophoresis (PFGE). Resistance to ciprofloxacin was found in 29% and 15% of isolates from retail meats and humans. We observed 61 PFGE profiles using two enzymes (SmaI, KpnI) with an Index of discrimination of 0·99, whereas MLST generated 37 sequence types. Two clonal complexes were identified with 58 (82%) C. coli isolates clustered in the ST‐828 complex. Conclusions: Resistance to ciprofloxacin and erythromycin was identified in C. coli obtained from retail meats and ill humans. PFGE typing of C. coli isolates was more discriminatory than MLST. Grouping of C. coli isolates (82%) by MLST in ST‐828 clonal complex indicates a common ancestry. Significance and Impact of the Study: A high frequency of resistance found to ciprofloxacin and erythromycin is concerning from food safety perspective. PFGE using single or double restriction enzymes was found to be more discriminatory than MLST for genotyping C. coli. Overall, the C. coli populations recovered from humans and retail meats were genotypically diverse.

Genetic Similarity ofCampylobacterIsolates in Humans, Food, and Water Sources in Central Poland

“…This study confirmed earlier observations that molecular genotyping of Campylobacter requires digestion with two endonucleases, that is, SmaI and KpnI (Thakur et al, 2009). Clusters A and D J were differentiated into two subclusters after analysis of PFGE results of KpnI digests.…”

Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli Isolates from Domestic and Travel-Associated Human Cases

“…The C. jejuni isolates showed much higher diversity than the C. coli isolates, as indicated by the distribution of the strains within different clonal complexes (CCs). Of the investigated C. coli strains, 95.0% could be assigned to CC828, confirming a higher clonality within this species (17,24,26).…”

Antimicrobial Resistance of Campylobacter Isolates from Retail Meat in the United States between 2002 and 2007

“…At NARMS, we continue to employ PFGE to characterize isolates at the genomic level, where PFGE correlated well with species. As shown by others (33,35,38), the use of a second enzyme increases the discriminatory power of PFGE. This is particularly important for outbreak investigations and for tracing etiologic agents to their sources, especially for isolates that do not cut with SmaI.…”