Genotyping of <i>Porcine Teschovirus</i> from Nervous Tissue of Pigs with and without Polioencephalomyelitis in Indiana

Bangari, Dinesh S.; Pogranichniy, Roman M.; Gillespie, Tom; Stevenson, Gregory W.

doi:10.1177/104063871002200415

Cited by 21 publications

(16 citation statements)

References 10 publications

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“…In addition to enteroviruses, sapeloviruses and teschoviruses, many picornaviruses have been detected in pigs: Foot-and-mouth disease viruses, encephalomyocarditis viruses, porcine cosaviruses, kobuviruses, pasiviruses, senecaviruses and tottoriviruses. The genus Teschovirus has been frequently associated with neurologic disease in pigs [2][3][4][5]. Porcine teschovirus (PTV) currently comprises 13 known serotypes [6][7][8], which is likely to expand with the continuous discovery of novel genotypes [9,10].…”

Section: Introductionmentioning

confidence: 99%

Two novel porcine teschovirus strains as the causative agents of encephalomyelitis in the Netherlands

et al. 2020

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Background: Porcine teschovirus (PTV) circulates among wild and domesticated pig populations without causing clinical disease, however neuroinvasive strains have caused high morbidity and mortality in the past. In recent years, several reports appeared with viral agents as a cause for neurologic signs in weanling and growing pigs among which PTV and new strains of PTV were described.Case presentation: On two unrelated pig farms in the Netherlands the weanling pig population showed a staggering gate, which developed progressively to paresis or paralysis of the hind legs with a morbidity up to 5%. After necropsy we diagnosed a non-suppurative encephalomyelitis on both farms, which was most consistent with a viral infection. PTV was detected within the central nervous system by qPCR. From both farms PTV full-length genomes were sequenced, which clustered closely with PTV-3 (98%) or PTV-11 (85%). Other common swine viruses were excluded by qPCR and sequencing of the virus. Conclusion:Our results show that new neuroinvasive PTV strains still emerge in pigs in the Netherlands. Further research is needed to investigate the impact of PTV and other viral agents causing encephalomyelitis within wild and domestic pig populations supported by the awareness of veterinarians.

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Section: Introductionmentioning

confidence: 99%

Two novel porcine teschovirus strains as the causative agents of encephalomyelitis in the Netherlands

et al. 2020

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“…Since then, the disease spreads throughout Europe and other continents during the 1940s and 1950s, causing devastating economic losses. At present, a mild form of the disease, with the exception of an outbreak in 2009 in the Republic of Haiti, has been described in many countries (Bangari, Pogranichniy, Gillespie, & Stevenson, ; Deng et al., ; Knowles, ; Pinto, Ankers, Werthmann, & Hamilton, ; Salles et al., ; Takahashi, Seimiya, Seki, & Yamada, ; Wang et al., ; Yamada et al., ; Zhang et al., ). The first documented PTV isolate (Swine/CH/IMH/03) in China was identified in 2003, and some cases and epidemiological studies related to PTV in China have been reported since then (Feng et al., ; Lin et al., ; Qiu, Wang, Zhang, Zhang, & Cui, ).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and molecular characterization ofPorcine teschovirusin Hunan, China

Yang

Luo

Yan

et al. 2017

Transbound Emerg Dis

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Porcine teschoviruses (PTVs) have been shown to be widely distributed in pig populations. In this study, 261 faecal and 91 intestinal content samples collected from pigs at 29 farms in Hunan, China, were tested for the presence of PTV by reverse transcription-polymerase chain reaction (RT-PCR). An overall PTV-positivity rate of 19.03% was detected by RT-PCR, and a high PTV infection rate was circulating in asymptomatic fattening and nursery pigs. In total, 40 PTV isolates (PTV-HuNs) were obtained. Alignment of their coding sequences with those of other known PTVs revealed that the genomic sequence of the polyprotein contains 6,606-6,621 nucleotides, encoding a 2,202-2,207-amino acid sequence. Phylogenetic analyses based on the VP1 gene and capsid protein gene exhibited 13 main lineages corresponding to PTV serotypes 1-13, and seven PTV serotypes (PTV 2-6, 9, and 11) were identified in the isolates obtained in our study; this is the first report of PTV 5, 9 and 11 in China. Recombination analysis among the PTV-HuNs indicated that nine recombination events have occurred, including both inter- and intraserotype events. In addition, results demonstrated that only limited positive selection is acting on the global population of PTV isolates, and purifying selection is predominant. In conclusion, this study revealed a high infection rate of PTVs circulating in asymptomatic fattening and nursery pigs. The 40 PTV-HuNs showed high genetic diversity, and genetic analysis of all available PTV sequences revealed that strong purifying selection and recombination play important roles in the genetic diversity and evolution of the virus.

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“…However, depending on the virus serotype and infection conditions, these picornaviruses may be considered important etiological agents of enteric, respiratory, reproductive, or neurological disorders (Bangari et al 2010, Ventura et al 2013. Clinical manifestations may include diarrhea, pneumonia, febrile illness, vesicular diseases, reproductive failure (SMEDI syndrome), myocarditis, and polioencephalomyelitis (Krumbholz et al 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Studies performed in different geographical regions throughout the world revealed that PTV, PSV, and EV-G are ubiquitous and have been reported in Asia (Kaku et al 2007, Lan et al 2011, Lin et al 2012, Moon et al 2012, Zhang et al 2012, Europe (Zell et al 2000, La Rosa et al 2006, Buitrago et al 2010, Sozzi et al 2010, Boros et al 2011, Prodělalová 2012, and the Americas (Bangari et al 2010, Salles et al 2011, Shan et al 2011, Deng et al 2012, Ventura et al 2013, Donin et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

et al. 2015

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RESUMO.-[Estudo molecular de teschovírus suíno, sapelovírus suíno e enterovírus G em javalis (Sus scrofa scrofa) de cativeiro no Paraná.] Teschovírus suíno (PTV), sapelovírus suíno (PSV) e enterovírus G (EV-G) são agentes infecciosos específicos da espécie suína que estão endemicamente disseminados em todo o mundo. O objetivo deste estudo foi investigar a infecção natural por estes picornavírus entéricos suínos em javalis (Sus scrofa scrofa) do estado do Paraná, Brasil e avaliar pecaris (Pecari tajacu e Tayassu pecari) como hospedeiros alternativos para estes vírus. Amostras fecais (n=36) de javalis assintomáticos (n=22) com idades de 2 a 7 meses (jovens, n=14) e 2 a 4 anos (adultos, n=8) e de pecaris (6 a 8 meses de idade, n=14) foram coletadas em um cativeiro e zoológico, res- Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric picornaviruses in wild boars (Sus scrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5'non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87

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Genotyping of Porcine Teschovirus from Nervous Tissue of Pigs with and without Polioencephalomyelitis in Indiana

Cited by 21 publications

References 10 publications

Two novel porcine teschovirus strains as the causative agents of encephalomyelitis in the Netherlands

Two novel porcine teschovirus strains as the causative agents of encephalomyelitis in the Netherlands

Epidemiology and molecular characterization ofPorcine teschovirusin Hunan, China

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

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