Genotyping of the JC virus in urine samples of healthy Korean individuals

Jeong, Byung‐Hoon; Lee, Kyung‐Hee; Choi, Eun-Kyoung; Kim, Kyunghoon; Kim, Yong‐Sun

doi:10.1002/jmv.10568

Cited by 32 publications

(38 citation statements)

References 48 publications

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“…Whether cross-virus interference may occur in the PCR amplification of dual infected samples is unclear. JCV was not detected among the samples tested, and this is consistent with previous reports of low JCV excretion rates (<5%) among individuals less than 20 years old [Chang et al, 2002;Ling et al, 2003;Jeong et al, 2004]. This is probably due, in large part, to the timing of JCV infection, which tends to be more common during the second and third decades of life.…”

Section: Discussionsupporting

confidence: 91%

Detection of BK virus and simian virus 40 in the urine of healthy children

Vanchiere¹,

White

Butel

2005

J. Med. Virol.

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Seroprevalence studies indicate that most primary infections with BK virus (BKV) and JC virus (JCV) occur in the first and second decades of life, respectively. Relatively little is known about the transmission of these agents, including the primary source of human exposure, the portal of entry, and the pathophysiology of life-long viral persistence. We sought to determine if simian virus 40 (SV40) excretion could be detected in the urine of healthy children and to define the age-related prevalence of polyomavirus shedding in this population. A point prevalence study of polyomavirus shedding was conducted in healthy children using rigorous enrollment criteria. Urine samples were collected from healthy children, age from 3 to 18 years, during routine evaluation at two urban pediatric clinics. Qualitative PCR analysis was performed using primers that detect a conserved region of the T-antigen gene of BKV, JCV, and SV40. The identity of polyomaviruses detected was determined by DNA sequence analysis and/or PCR amplification of other regions of the viral genomes. Seven of 72 (9.7%) urine samples were positive for polyomaviruses: three with BKV (ages 4, 6, 13), two with SV40 (ages 6, 16), two with BKV and SV40 co-excretion (ages 6, 15), and none with JCV. DNA sequence analysis confirmed the identity of viruses detected. These results suggest that the timing of SV40 infections in humans may be similar to that of BKV and that urine from healthy children could contribute to the ubiquity of BKV infection early in life.

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Section: Discussionsupporting

confidence: 91%

Detection of BK virus and simian virus 40 in the urine of healthy children

Vanchiere¹,

White

Butel

2005

J. Med. Virol.

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“…The median JC virus genome copy number was 3.4 times higher among subjects in the HIV-seropositive group. In contrast to other studies [8,9], JC virus detection in the urine was more common among younger subjects. In our study, no correlation was found between CD4 + cell count and JC virus copy number.…”

contrasting

confidence: 81%

JC Virus Detection in Bodily Fluids: Clues to Transmission

Berger¹,

Miller²,

Mootoor³

et al. 2006

CLIN INFECT DIS

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JC virus in saliva, oropharyngeal fluid, blood, and urine samples obtained from 58 human immunodeficiency virus-infected persons and 58 matched controls was investigated by performing quantitative polymerase chain reaction. JC virus was rarely present in oropharyngeal fluid and blood samples, even in those obtained from immunosuppressed individuals, but it was commonly detected in urine samples from both groups, suggesting that urine contributes to transmission.

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“…Such results were reported previously in a healthy group; and this may be due to the physiological differences that occur between males and females [18,28].…”

Section: Discussionsupporting

confidence: 68%