Gentamicin and bone morphogenic protein-2 (BMP-2)-delivering heparinized-titanium implant with enhanced antibacterial activity and osteointegration

Antimicrobial dental implant functionalization strategies —A systematic review

“…The combination of gentamycin and bone-morphogenic-protein-2 was tested for the dual function of antimicrobial activity and osteoblast function. The results showed enhanced alkaline phosphatase activity and calcium deposition by osteoblast cells 27) . Antibiotic-releasing surfaces were non-toxic to osteoblast cells and showed good cytocompatibility compared to uncoated titanium 21,31,36) .…”

“…Chlorhexidine (CHX)-loaded titanium surfaces reduce colonization with streptococci and S. aureus in comparison to titanium control [24][25][26] . Furthermore, there have been in vitro studies on the antibacterial effect of dual drug-delivering systems, combining antibiotics with osteoconductive drugs; these have been found to reduce biofilm formation in comparison to titanium control 27) . Interestingly, two studies investigated molecules as anti-biofilm coatings that were not initially intended to be antimicrobial substances.…”

How to use BMP-2 for clinical applications? A review on pros and cons of existing delivery strategies

“…In vitro tests showed reduced inflammatory potential as analyzed in a murine macrophage cell line, a sustained release of BMP-2 from the heparin-titanium discs and stimulated osteoblast function which was further proven by significantly higher ALP activities and calcium contents in cells grown on BMP-2 (50 ng)-immobilized titanium surfaces. Similar approaches to functionalize titanium surfaces were performed by Lee et al and reported in 2012 [100].…”

Multifunctional biomaterial coatings: synthetic challenges and biological activity

“…In most of the approaches electrostatic interactions are used to graft GAGs on positively charged peptides or proteins as collagen (Wieduwild et al, 2013;Miron et al, 2014). Other strategies covalently link GAGs by their functionalities like carboxyl and amino groups (Kim et al, 2011;Lee et al, 2012;Ao et al, 2013). Anchored in the cell membrane, proteoglycans (e.g.…”