Gentamicin Nephrotoxicity in Rat: Some Biochemical Correlates

Ali, Badreldin H.; Gayoum, A.A. Abdel; Bashir, A.A.

doi:10.1111/j.1600-0773.1992.tb00500.x

Cited by 39 publications

(20 citation statements)

References 28 publications

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“…Researchers also observed that creatinine (Cr) level is a more accurate measurement for glomerular impairment than urea levels/Cr clearance. [42,43] Our study results coincide with earlier reports that a significant increase in plasma urea and creatinine or decrease in potassium levels [40,41,44] might indicate a nephrotoxic condition in GEN-treated rats and may be due to kidney damage caused by the enhanced generation of ROS. These findings correlated well with the renal histological examination, which revealed renal tubular necrosis and interstitial nephritis.…”

Section: Discussionsupporting

confidence: 82%

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Jeyanthi

Subramanian

2009

Renal Failure

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In the present study, we investigated the protective effect of Withania somnifera, an indigenous medicinal herb used in ayurvedic traditional systems for more than 3000 years in India, on gentamicin (GEN)-induced nephrotoxicity. The root extract of three different doses of W. somnifera (viz., 250, 500, and 750 mg/kg) was administered orally to rats for 14 days before GEN treatment and thereafter concurrently with GEN (100 mg/kg) for 8 days. Nephrotoxicity was evident in GEN-treated rats by significant increase in kidney weight, urea, creatinine, urinary protein, and glucose, and significant reduction in body weights and potassium, which was histopathologically confirmed by tubular necrosis. In contrast W. somnifera (500 mg/kg) significantly reversed these changes as evidenced microscopically when compared to other two doses of W. somnifera (250 and 750 mg/kg), and there were no significant changes in the levels of sodium in the experimental animals compared to control. Thus, our results suggested the nephroprotective effect of Withania somnifera, which could be by enhancing antioxidant activity with natural antioxidants and scavenging the free radicals.

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Section: Discussionsupporting

confidence: 82%

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Jeyanthi

Subramanian

2009

Renal Failure

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“…GM nephrotoxicity is, at least partially, attributed to the generation of free radicals, enhanced lipid peroxidation, and decreased glutathione (GSH), in the kidney (Ramsammy et al, 1985;Walker & Shah, 1988;Ali et al, 1992). It is reasonable, therefore, to suggest that the observed nephroprotective action of the date flesh and pits may be due to an increase in activity of the free radical scavenging enzymes or counteraction of the free radicals.…”

Section: Discussionmentioning

confidence: 99%

Nephroprotective Action ofPhoenix dactylifera. in Gentamicin-Induced Nephrotoxicity

Alqarawi

Abdel-Rahman

Mousa

et al. 2008

Pharmaceutical Biology

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We investigated the effect of an extract of the flesh and pits of dates [Phoenix dactylifera L (Arecaceae or Palmae)] on gentamicin (GM) nephrotoxicity in rats. The animals were given either the date flesh extract mixed with the food (50% w/w) or the pits extract mixed in the drinking water (2:1 w/v), and GM (80 mg kg −1 day −1 intramuscularly for 6 days) was injected during the last 6 days of treatment. Other groups of rats were given GM concomitantly with the date flesh extract or the date pits extract at the above doses. GM treatment significantly increased the plasma concentrations of creatinine and urea and induced a marked necrosis of the renal proximal tubules. The date flesh and pits were effective in significantly reducing the increases in plasma creatinine and urea concentrations induced by GM nephrotoxicity and ameliorating the proximal tubular damage. Antioxidant components in the date (e.g., melatonin, vitamin E, and ascorbic acid) were suggested to be the basis of the nephroprotection.

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“…An understanding of the antibiotic's nephrotoxicity therefore has substantial clinical significance [1]. But early detection of renal damage is difficult.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of KIM-1 and NGAL as Early Indicators for Assessment of Gentamycin-Induced Nephrotoxicity In Vivo and In Vitro

Luo

Chen

et al. 2016

Kidney Blood Press Res

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Background/Aims: The aminolycoside Gentamicin is a widely used antibiotic, applied in equine medicine. Despite its clinical use, concerns remain regarding the potential toxic side-effects, such as nephrotoxicity. Early detection of renal damage is critical in preclinical drug development. This study was aimed to determine whether kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) may be early indicators in the assessment of Gentamycin-induced nephrotoxicity. Methods: In our study, a model of Gentamicin-induced nephrotoxicity in male Sprague Dawley rats treated for up to 7 days at 50 or 100mg/kg/day was used to monitor the expressions of novel biomarkers of renal toxicity during the progression of acute kidney injury (AKI). Additionally, biomarkers were assessed in human kidney proximal epithelial cells (HK-2) treated with Gentamicin for 2, 6, 12, 24, 36 or 48h in vitro. Results: Repeated administration of Gentamicin to rats for 1, 3, or 7 days resulted in a dose- and time-dependent increase in the expression of KIM-1 and NGAL. The expressions of the two biomarkers changed prior to renal tubule damage and increases in serum creatinine (SCr) and blood urea nitrogen (BUN) levels, suggesting their usefulness for predicting Gentamicin-induced acute kidney injury (AKI) in vivo. Conclusions: In contrast, no significant increase in the expression of the biomarker genes and proteins were evident in HK-2 cells after treated by Gentamycin for up to 48h, suggesting that they may not be suitable endpoints for sensitive detection of nephrotoxic effects in vitro.

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Gentamicin Nephrotoxicity in Rat: Some Biochemical Correlates

Cited by 39 publications

References 28 publications

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Nephroprotective Action ofPhoenix dactylifera. in Gentamicin-Induced Nephrotoxicity

Evaluation of KIM-1 and NGAL as Early Indicators for Assessment of Gentamycin-Induced Nephrotoxicity <b><i>In Vivo</i></b> and <b><i>In Vitro</i></b>

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