Geobiochemistry characteristics of rare earth elements in soil and ground water: a case study in Baotou, China

Tang, Shuting; Zheng, Chunli; Chen, Minjie; Du, Weiqi; Xu, Xin

doi:10.1038/s41598-020-68661-4

Cited by 26 publications

(11 citation statements)

References 50 publications

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“…The DNA extract was checked on a 1% agarose gel, and the DNA concentration and purity were determined with a NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Wilmington, United States). The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified with the primer pair 338F (5 -ACTCCTACGGGAGGCAGCAG-3 ) and 806R (5 -GGACTACHVGGGTWTCTAAT-3 ) by an ABI GeneAmp R 9700 PCR thermocycler (ABI, CA, United States), with an eight-base sequence barcode unique to each sample at the 5' end of 338F and 806R (Tang et al, 2020). PCR amplification of the 16S rRNA gene was performed as follows: initial denaturation at 95 • C. The PCR mixtures contained 5 × TransStart FastPfu buffer (4 µL), 2.5 mM dNTPs (2 µL), 5 µM forward primer (0.8 µL), 5 µM reverse primer (0.8 µL), TransStart FastPfu DNA Polymerase (0.4 µL), template DNA (10 ng), and ddH2O (up to 20 µL).…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Alteration of Gut Microbiota in Patients With Epilepsy and the Potential Index as a Biomarker

et al. 2020

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Objective: To explore the structure and composition of the fecal microbiota of patients with epilepsy. Methods: Variations in the fecal microbiota between patients with epilepsy and healthy controls (HCs) from the same household were investigated and validated by utilizing 16S ribosomal RNA sequencing in two independent cohorts [exploration cohort (N = 55 patients and N = 46 HCs) and validation cohort (N = 13 patients and N = 10 HCs)]. Results: The alpha diversity indexes of the specimens from patients with epilepsy were much lower than those from the HCs (p < 0.05). The structure and composition of the fecal microbiota differed between patients with different clinical prognoses and between patients and HCs (Adonis: p < 0.05). Microbiome alterations in patients with epilepsy included increases in Actinobacteria and Verrucomicrobia and decreases in Proteobacteria at the phylum level and increases in Prevotella_9, Blautia, Bifidobacterium, and others at the genus level [linear discriminant analysis (LDA): 3.5] Patients with drug-resistant epilepsy showed enrichment of bacterial taxa in Actinobacteria, Verrucomicrobia, and Nitrospirae and the genera Blautia, Bifidobacterium, Subdoligranulum, Dialister, and Anaerostipes (Kruskal-Wallis test: p < 0.05). Analysis of gut microbiome indicated predictive ability for disease diagnosis, with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.97 (95% CI, 0.84-0.98). Applying the model to our validation cohort resulted in an AUC of 0.96 (95% CI, 0.75-0.97). Notably, the model could distinguish drug-resistant from drug-sensitive epilepsy (AUC = 0.85, 95% CI: 0.69-0.94). Conclusion: Patients with epilepsy exhibit substantial alterations of fecal microbiota composition, and specific gut commensal strains are altered depending on different clinical phenotypes and thus could serve as potential biomarkers for disease diagnosis.

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Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Alteration of Gut Microbiota in Patients With Epilepsy and the Potential Index as a Biomarker

et al. 2020

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“…In addition, the aluminosilicates in Al-rich CFAs may also host REYs. Another possibility is that, to some extent, Mn(II) can also form hydroxides or oxides of ferromanganese with the EPR spectral feature of type (c) to adsorb or complex REYs. − …”

Section: Resultsmentioning

confidence: 99%

Fast Screening of Coal Fly Ash with Potential for Rare Earth Element Recovery by Electron Paramagnetic Resonance Spectroscopy

Liu

Han

et al. 2021

Environ. Sci. Technol.

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Rare earth elements (REYs) are in increasing global demand, but their mining is costly and environmentally destructive. Coal fly ash (CFA) is a promising alternative source of REYs, but it is necessary to identify CFA with sufficiently high REY concentrations. This study proposes the use of electron paramagnetic resonance (EPR) spectroscopy as part of a simple method to identify CFAs with adequate REY concentrations. The EPR spectra of CFA samples taken from 186 Chinese commercial coal-fired power plants were analyzed. The results suggest that CFAs without evident 6-fold resonances are worth recycling (REY concentrations of 416 ± 108 mg/kg), while those with conspicuous 6-fold resonances are not worth recycling (REY concentrations of 55 ± 26 mg/kg). This is probably due to isomorphic substitution of Ca(II) for Mn(II) and REY(III), resulting in low concentrations of Mn(II) and REY(III) in Ca-rich CFAs. This EPR evaluation method does not require specialized sample preparation, professional skills, or secondary data analysis and has potential global significance in the fast screening of CFAs with REY-recycling potential.

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“…8 The genetic types and chemical nature of REEs cause the fractionation of REEs in the coal seam. 9,10 The heavy REEs (HREEs) including Eu, Tb, Gd, Y, Lu, Dy, Tm, Ho, and Er are reported to be present at high concentration in the coals with low ash content, whereas their concentrations are low in the coals with high ash content. 11 Desorption and leaching of HREEs from tonsteins, clays, and other rocks are higher than light REEs (LREEs).…”

Section: Introductionmentioning

confidence: 99%

Bio-weathering Using Shewanella oneidensis MR-1 Enhances Selective Recovery of Rare Earth Elements from Alaskan Coal Mines

Sachan

Dev

Briggs

et al. 2022

Preprint

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Coal mines in Alaska with high rare earth elements (REEs) levels (286-524 mg/kg) serve as an alternative domestic source for REEs. Existing leaching/separation technologies fail to selectively recover REEs from the feedstock and require downstream multiple purification stages that increase the overall operational cost. This study aims at bio-weathering coal from two Alaskan coal mines (Wishbone Hill and Healy) at three density fractions (1.3 float, and 1.3 and 1.5 sink) using Shewanella oneidensis MR-1 for achieving higher selective REEs recovery in one-step process. Optimizing the bio-weathering process by varying solids percentages (5.7 to14.3% w/v), particle size (-14 to -200 M), incubation temperatures (30 to 34 °C), and inoculum dosing (0.2 to 1% v/v) resulted in highest recovery of Neodymium (75.3%) and total REEs (98.4%) from 1.3 float Wishbone Hill and 1.3 sink Healy coal, respectively. When compared to the chemical leaching process, bio-weathering enhanced selective recovery of REEs including Scandium, Yttrium, Ytterbium, Terbium, Erbium, and Lutetium from Healy coal at low density, and Yttrium from Wishbone Hill coal at high density. The results indicate the future scope for developing cost-effective selective REEs recovery processes that may address the global critical minerals supply chain risk.

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Geobiochemistry characteristics of rare earth elements in soil and ground water: a case study in Baotou, China

Cited by 26 publications

References 50 publications

Alteration of Gut Microbiota in Patients With Epilepsy and the Potential Index as a Biomarker

Alteration of Gut Microbiota in Patients With Epilepsy and the Potential Index as a Biomarker

Fast Screening of Coal Fly Ash with Potential for Rare Earth Element Recovery by Electron Paramagnetic Resonance Spectroscopy

Bio-weathering Using Shewanella oneidensis MR-1 Enhances Selective Recovery of Rare Earth Elements from Alaskan Coal Mines

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