Geochemical characterization and renal cell toxicity of water-soluble extracts from U.S. Gulf Coast lignite

Ojeda, Ann; Ford, S. D.; Gallucci, Randle M.; Ihnat, Michael A.; Philp, R. Paul

doi:10.1007/s10653-018-0196-7

Cited by 8 publications

(7 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Excessive generation of ROS and ensuing oxidative damage has been implicated in human or animal cells exposed to PM 2.5 [10][11][12]. ROS is composed of superoxide anion radical, hydroxyl radical, peroxy radicals and nitric oxide radicals, etc.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we chose human proximal tubule epithelial cell as a vitro model to investigate these signaling pathways triggered by PM 2.5 . The human kidney cell line HK-2 was first established in 1994 by Dr. Ryan's laboratory and has been widely used in experiments on nephrology and nephrotoxicity [12,28,29]. Moreover, HK-2 cells have been found to exhibit most of the functional characteristics of proximal tubular tissues in human kidneys [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The activation of antioxidant and apoptosis pathways involved in damage of human proximal tubule epithelial cells by PM2.5 exposure

et al. 2020

View full text Add to dashboard Cite

Background: Exposure to airborne fine particulate matter (PM 2.5 ) has been reported to be harmful to the human kidney. However, whether the activation of oxidative stress and cell apoptosis plays key roles in the nephrotoxicity caused by PM 2.5 exposure is still poorly understood. The aim of this study was to explore the mechanism of cytotoxicity after PM 2.5 exposure in human proximal tubule epithelial cells (HK-2 cells).Results: PM 2.5 exposure resulted in a significant decrease in cell viability, with an increase in LDH release and the early kidney damage marker kidney injury molecule-1 (KIM-1) expression in a dose-dependent manner and timedependent manner. PM 2.5 exposure induced reactive oxygen species (ROS) generation and markedly elevated apoptosis in HK-2 cells. In addition, PM 2.5 exposure resulted in the activation of antioxidant pathway, as evidenced by the increased expressions of Nrf2, HO-1 and NQO1 and decreased expression of Keap1. Moreover, PM 2.5 exposure also induced the activation of apoptotic pathway, as evidenced by the increased expressions of pro-apoptotic proteins Bax, caspase-3 and caspase-8 and decreased expression of antiapoptotic protein Bcl-2. Conclusions:Our results demonstrated that both antioxidant pathway and apoptotic pathway played critical roles in the damage mediated by PM 2.5 in HK-2 cells. This study would give us a strategy to prevent the impairment of renal function by PM 2.5 induced through repression of oxidative stress and apoptosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The activation of antioxidant and apoptosis pathways involved in damage of human proximal tubule epithelial cells by PM2.5 exposure

et al. 2020

View full text Add to dashboard Cite

show abstract

“…U.S.A) which is called Pan-endemic Nephrophaty (PEN; Orem et al, 2007). There are numerous hypotheses for BEN aethiology such as exposure to toxins (Grollman & Jelaković, 2007;Lukinich-Gruia et al, 2022;Pfohl-Leszkowicz, et al, 2002;Stiborová et al, 2016, Stoev, 2017, to organic compounds leached from low-rank coal deposits (mainly lignites; Feder et al, 1991;Ojeda et al, 2019) and deficiency of some metals. However there are still some debates, the Pliocene lignite hypothesis is a plausible hypothesis mainly because of the high correlation between lignite deposit locations and the BEN-endemic villages (Voice et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Organic and Inorganic Contaminants in Water Sources around Elbistan Lignite Beds

SELVER>

Uras

2022

Gazi University Journal of Science Part A: Engineering and Innovation

View full text Add to dashboard Cite

The household and industrial use, as well as mining of coal, pose various environmental and health risks including lung and kidney diseases such as Balkan Endemic Nephropathy (BEN). BEN is a kidney disease and it is geographically confined to Balkan countries where extensive lignite deposits are located. The most accepted cause of BEN is the use of untreated waters contaminated by lignite-derived organic contaminants. Afşin-Elbistan basin contains approximately 50 % of the lignite reserves of Turkey which makes it an ideal location for the current study. In this work, water samples were taken from 10 different locations in the Afşin-Elbistan basin and they were analyzed for organic and inorganic contaminants. Results showed that none of the water samples were contaminated with inorganic contaminants and indeed met the Turkish drinking water standards. GC-MS analyses of the water samples revealed similar chromatograms. Briefly, all the water samples have similar organic compound types such as n-alkanes, chlorophyll-derived phytols, and plant- and animal-derived acids (i.e. palmitic acid). On the other hand, none of the samples were contaminated with carcinogenic and/or nephrotoxic organic compounds such as polyaromatic hydrocarbons and aromatic amines, which is contrary to many, but not all of the previous works conducted in Balkan countries. All these results may indicate that the influence of coal deposits on the groundwaters is minimal.

show abstract

“…Certain phenols were associated with kidney disease 1 and disruption of the endocrine system by estrogenic activity. 2 Phenolic compounds in aqueous systems can, on one hand, arise from natural processes, for example, lignite leachates 1 or decomposition of plants. On the other hand, aqueous systems are polluted with phenolic compounds by industrial and agricultural practices, for example, synthetic resin and plastic waste leachates, dye manufacturing, and degradation of chlorophenoxycarboxylic herbicides and organophosphorous insecticides.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Phenolic compounds are substances of major environmental concern due to their toxicity and high solubility in water (phenol, 83 g/L; cresol isomers, 22−26 g/L). Certain phenols were associated with kidney disease 1 and disruption of the endocrine system by estrogenic activity. 2 Phenolic compounds in aqueous systems can, on one hand, arise from natural processes, for example, lignite leachates 1 or decomposition of plants.…”

Section: ■ Introductionmentioning

confidence: 99%

Analysis of Carbon and Hydrogen Stable Isotope Ratios of Phenolic Compounds: Method Development and Biodegradation Applications

et al. 2021

View full text Add to dashboard Cite

Phenolic compounds are priority water pollutants of both natural and anthropogenic origins. Distinguishing the sources and degradation pathways of phenolic compounds in the environment is essential for sustainable water management. In this study, we have developed and validated methods based on gas chromatography−isotope ratio mass spectrometry for analysis of hydrogen and carbon isotope ratios of phenol and cresols. For carbon stable isotope analysis, solid-phase extraction was applied, whereas liquid−liquid extraction, followed by derivatization with trifluoroacetic anhydride, was used for hydrogen stable isotope analysis. The methods were verified in biodegradation experiments using p-cresol and phenol as substrates. Methyl group oxidation of p-cresol by Pseudomonas pseudoalcaligenes and Aromatoleum aromaticum resulted in the strong hydrogen (−76 ± 2 and −103 ± 2 mUr, respectively) and varying carbon isotopic fractionation (−2.1 ± 0.1 and −1.0 ± 0.1 mUr, respectively), leading to distinguishable two-dimensional plots for hydrogen versus carbon isotope ratios, indicating slightly different enzymatic reactions. For anaerobic phenol degradation by Desulfosarcina cetonica, the absence of hydrogen isotopic fractionation indicates adenosine triphosphate-dependent carboxylation as the initial step of the biodegradation pathway. The methods developed in this study are useful to detect biological degradation of phenolic compounds in highly contaminated aqueous environmental systems.

show abstract

Geochemical characterization and renal cell toxicity of water-soluble extracts from U.S. Gulf Coast lignite

Cited by 8 publications

References 70 publications

The activation of antioxidant and apoptosis pathways involved in damage of human proximal tubule epithelial cells by PM2.5 exposure

The activation of antioxidant and apoptosis pathways involved in damage of human proximal tubule epithelial cells by PM2.5 exposure

Investigation of Organic and Inorganic Contaminants in Water Sources around Elbistan Lignite Beds

Analysis of Carbon and Hydrogen Stable Isotope Ratios of Phenolic Compounds: Method Development and Biodegradation Applications

Contact Info

Product

Resources

About