Geographic classification of dengue-2 virus strains by antigen signature analysis

Monath, Thomas P.; Wands, Jack R.; Hill, L.; Brown, Nancy V.; Marciniak, Robert A.; Wong, M. A.; Gentry, Mary K.; Burke, Donald S.; Grant, Joyce A.; Trent, Dennis W.

doi:10.1016/0042-6822(86)90457-5

Cited by 48 publications

(33 citation statements)

References 19 publications

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“…Antigenic variation among dengue virus isolates has been previously reported by Monath et al (17). By using a panel of MAb in the antigen signature analysis, a DEN-2 Burma (S-40916) strain was found to lack an antigenic epitope for the 3H5 type-specific MAb binding.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some strains of dengue viruses occasionally lack a specific epitope for certain typespecific monoclonal antibody (MAb) (17), therefore, using that MAb for the identification of such dengue virus isolates may lead to false-negative results. Polymerase chain reaction (PCR), a new technique for an in vitro amplification of specific DNA segments (19,25), offers several obvious advantages for the diagnosis of viral infections.…”

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confidence: 99%

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Applications of Polymerase Chain Reaction for Identification of Dengue Viruses Isolated from Patient Sera

Maneekarn

Morita

Tanaka

et al. 1993

Microbiology and Immunology

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A simple and sensitive procedure of reverse transcriptase polymerase chain reaction (RT-PCR) was developed previously such that all 4 serotypes of dengue viruses could be detected and their serotypes identified simultaneously in a single-step procedure. In this study we compared the RT-PCR with a conventional immunoperoxidase (PAP) staining method for the identification of dengue viruses currently isolated from patient sera. Sixty-six sera taken from dengue hemorrhagic fever (DHF) patients were subjected to virus isolation by inoculating onto C6/36 cell cultures. Screening for the presence of dengue viruses in culture fluids was done after 7 days of incubation by PAP staining using hyperimmune rabbit anti-dengue virus antibody as the primary reagent. Dengue viruses in positive cultures were further identified for their serotypes by PAP using type-specific monoclonal antibodies (MAb) and by RT-PCR. Thirty-two out of the 66 serum specimens tested (48.5%) were positive for dengue viruses. Of these, 5 were type 1 (DEN-1), 25 were type 2 (DEN-2) and 2 contained both DEN-1 and DEN-2. All cultures that were positive by PAP method were also positive by RT-PCR and vice versa. Thus, the results obtained by RT-PCR were in good agreement with those by PAP. It is important to point out that while all 5 DEN-1 isolates reacted readily with the MAb 1F1, only 2 of them could be identified by the MAb 15F3. Our data suggest that antigenic variation among DEN-1 isolates occur frequently and this should be taken into consideration in the selection of appropriate type-specific MAb for serotyping of dengue viruses. We also demonstrated dual infection of DEN-1 and DEN-2 in two patients' sera by RT-PCR.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Applications of Polymerase Chain Reaction for Identification of Dengue Viruses Isolated from Patient Sera

Maneekarn

Morita

Tanaka

et al. 1993

Microbiology and Immunology

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“…:~ Determined by HI testing; 1 °, primary; 2 °, secondary. § Defined as Thailand-Burma topotype by Monath et al (1986).…”

Section: Methodsmentioning

confidence: 99%

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Walker

Henchal

Blok

et al. 1988

Journal of General Virology

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SUMMARYDengue type-2 viruses isolated in metropolitan Bangkok during 1980 (Bangkok/80) were characterized by oligonucleotide fingerprinting, restriction enzyme (RE) mapping and antigenic analysis using monoclonal antibody probes. Of 10 isolates analysed by oligonucleotide fingerprinting, nine were very closely related, showing 72-5 ~o to 91.4 ~ oligonucleotide homology. One isolate (D80-141) produced a distinctly different fingerprint (55.7~ to 58"0~o homology) and was less related to other Bangkok/80 dengue-2 virus isolates than to a 1964 Bangkok isolate (16681). RE mapping conducted on complementary dsDNA prepared from three Bangkok/80 isolates, strain 16681 and the prototype New Guinea C strain confirmed that D80-141 was genetically distinct. On antigenic analysis, only one of 22 monoclonal antibody probes produced against representative 1980 Bangkok dengue-2 isolates, D80-100 and D80-141, was able to distinguish between these virus strains. Monoclonal antibody 47-10/10, prepared using D80-100 virus and directed at the NS1 non-structural glycoprotein, had a significantly lower (100-fold) solid phase radioimmune assay endpoint titre for D80-141 antigen than for D80-100 antigen. By the indirect immunofluorescence assay, 47-10/10 had lower antibody endpoint titres against D80-141, the NGC strain and 13 (12~o) of 110 Bangkok/80 isolates than to a control antibody preparation. These results suggest that strain D80-141 represents a second minor topotype of dengue-2 which was circulating concurrently with the major endemic topotype in Bangkok in early 1980.

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“…Monoclonal antibody signature analysis is quite sensitive, and it correlates well with analysis by oligonucleotide fingerprinting. While the technique is rapid, it requires that all strains to be compared should be run in a single assay, and thus is not as feasible for single virus isolations (Monath et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Oligonucleotide fingerprinting of virus isolates is slow, expensive, and limited to laboratories with highly trained personnel. Other approaches for detecting genetic variation include one-dimensional analysis of RNase T1 fragments (A. V. Vorndam, personal communication), restriction endonuclease analysis of random primed single-stranded cDNA (Faragher et al, 1985), primer extension sequence analysis of specific genome regions (Ghosh et al, 1980), monoclonal antibody signature analysis (Wands et al, 1981 ;Monath et al, 1986) and RNA/DNA hybridization using cDNA probes (Blok et al, 1984;Blok, 1985). We have developed a novel method of obtaining information by the construction of specific short DNA probes for the rapid detection by RNA/DNA hybridization of DEN 2 genetic variants.…”

Section: -7289mentioning

confidence: 99%

Genetic and Epidemiological Studies of Dengue Type 2 Viruses by Hybridization Using Synthetic Deoxyoligonucleotides as Probes

Kerschner¹,

Vorndam²,

Monath³

et al. 1986

Journal of General Virology

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SUMMARYA rapid nucleic acid hybridization procedure was developed for examining the genotypic variation of dengue type 2 viruses (DEN 2) having distinct RNase T1 fingerprints and isolated from different geographical areas. Synthetic DNA hybridization probes were constructed complementary in nucleotide sequence to coaimon and unique RNase TI oligonucleotides of topotype viruses from Puerto Rico/South Pacific, Jamaica, the Seychelles, Thailand/Burma and Africa. Hybridization probes with both type-and topotype-specific reactivities were observed, as were probes specific for two or more of the DEN 2 topotypes. These results confirm geographical movement of topotype virus strains and suggest possible origins. Detection of DEN 2 RNA by hybridization is a rapid and reproducible method that can be modified and applied as a viable alternative to the laborious T1 oligonucleotide fingerprinting.

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Geographic classification of dengue-2 virus strains by antigen signature analysis

Cited by 48 publications

References 19 publications

Applications of Polymerase Chain Reaction for Identification of Dengue Viruses Isolated from Patient Sera

Applications of Polymerase Chain Reaction for Identification of Dengue Viruses Isolated from Patient Sera

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Genetic and Epidemiological Studies of Dengue Type 2 Viruses by Hybridization Using Synthetic Deoxyoligonucleotides as Probes

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