Geographic distribution and phylogenetic analysis of cucurbit yellow stunting disorder virus in Iran

Abstract

Summary. -Cucurbit yellow stunting disorder virus (CYSDV) is a destructive virus of cucurbits in Iran. During 2008-2012 growing seasons a total of 366 cucurbit samples including melon, cucumber, snakemelon and squash with typical symptoms of CYSDV infection were collected from ten Provinces in Iran. They were screened by ELISA and the infectivity of ELISA-positive samples was confirmed by RT-PCR. The results showed that 309 out of 366 samples were infected by CYSDV. The virus was present in many areas of southern and central Provinces of the country. Analyses of nucleotide and amino acid sequences of the CYSDV coat protein (CP) showed that Iranian isolates form a cluster and were placed in the Eastern subgroup of CYSDV. The Eastern subgroup of CYSDV was divided into two diverged subgroups including Iranian isolates and Saudi Arabian isolates. The identity among Iranian isolates was more than 99 %. Estimation of genetic distances showed that the number of nucleotide and amino acid substitutions per site from averaging overall Iranian sequence pairs were 0.004 and 0.008, respectively. Phylogenetic analyses and the estimation of genetic distance indicated that Iranian isolates have low genetic diversity.Keywords: CYSDV; crinivirus; Closteroviridae; coat protein; sequence; Iran * Corresponding author: E-mail: shamsbakhsh@modares.ac.ir; phone: +9821-48292274. Abbreviations: CP = coat protein; CYSDV = cucurbit yellow stunting disorder virus; HSP70h = heat shock protein 70 homologue; ORF = open reading frame; TMGMV = tobacco mild green mosaic virus

Whitefly‐transmitted RNA viruses that affect intensive vegetable production

“…It was first described in the United Arab Emirates in 1982, where it was found causing a yellowing disease on cucurbit crops (Hassan & Duffus, ). In 1996, CYSDV was reported severely affecting melon and cucumber crops in Spain (Célix et al , ) and, afterwards, it was described in many other regions of the world where intensive cultivation of cucurbits takes place (Wisler et al , ; Abou‐Jawdah et al , ; Desbiez et al , , ; Kao et al , ; Louro et al , ; Boubourakas et al , ; Wintermantel et al , ; Yakoubi et al , ; Herrera‐Vásquez et al , ; Keshavarz et al , ). In Spain, CYSDV has become one of the prevalent viruses in protected cucurbit crops of south‐eastern regions, where up to 100% of plants are frequently infected (Célix et al , ; Kassem et al , ; Juárez et al , ).…”

Identification of viruses infecting cucurbits and determination of genetic diversity of Cucumber mosaic virus in Lorestan province, Iran

“…CYSDV is a destructive cucurbit virus in large areas of the world that reduces the yield from 30 to 50 percent (Louro et al 2000). Th is virus has spread in recent years, and has been found in many regions of Iran (Keshavarz et al 2013b). CCYV is a new whitefl y-transmitted crinivirus which causes serious harm to melon and cucumber crops.…”

First report of Cucurbit yellow stunting disorder virus infecting cucurbits in Italy

“…Phylogenetic analysis of the CP gene sequence showed that the Italian isolate clustered with isolates from different countries in the Mediterranean basin and from the American continent within the "Western" group (Fig. 3) which is phylogenetically distant from the "Eastern" group including isolates from Iran, Saudi Arabia and Sudan (Rubio et al, 2001;Keshevarz et al, 2013).…”