1985
DOI: 10.1093/infdis/152.5.979
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Geographic Variation Among Isolates of Trichomonas vaginalis: Demonstration of Antigenic Heterogeneity by Using Monoclonal Antibodies and the Indirect Immunofluorescence Technique

Abstract: Although Trichomonas vaginalis causes one of the most common sexually transmitted diseases, little is known about the antigenic variation of the parasite or about differences between strains in epidemiology or virulence. Variation among isolates of T. vaginalis was investigated by using a panel of monoclonal antibodies, each reactive with different antigens, to test 88 isolates from diverse geographic areas of North America. All isolates of T. vaginalis reacted with at least one of the nine monoclonal antibodi… Show more

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Cited by 62 publications
(43 citation statements)
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“…This approach, although very useful strains in vitro; furthermore, every isolate is different with regard to the extent of its phagocytic activity. Similar variability has been found for other protozoan characteristics, such as the cytopathic effect, adhesiveness to cell substrates, antigenic composition of the cell surface and binding to tectins (11)(12)(13). It is of note that some preliminary experiments also showed quantitative variations in the killing activity of different Trichomonas vaginalis isolates (data not shown).…”
Section: Discussionsupporting
confidence: 76%
“…This approach, although very useful strains in vitro; furthermore, every isolate is different with regard to the extent of its phagocytic activity. Similar variability has been found for other protozoan characteristics, such as the cytopathic effect, adhesiveness to cell substrates, antigenic composition of the cell surface and binding to tectins (11)(12)(13). It is of note that some preliminary experiments also showed quantitative variations in the killing activity of different Trichomonas vaginalis isolates (data not shown).…”
Section: Discussionsupporting
confidence: 76%
“…29 The reaction-profiles of 88 isolates to a battery of monoclonal antibodies identified 11 distinct patterns; some groups contained isolates from five disparate geographic sites and one geographic site contained representatives of all 11 groups. 27 In the same study, identical profiles obtained for the ATCC reference strains and for isolates collected 25 years later, also suggest a stable antigenic composition of some Trichomonas populations over time. A general theory of clonal reproduction has been proposed for parasitic protozoa, and those clones appear to be stable across large geographical areas and over long periods of time.…”
Section: Discussionsupporting
confidence: 55%
“…14,[27][28][29] Isoenzyme characterization based on the electrophoretic patterns of four enzymes, classified 32 Trichomonas isolates into five groups, 28 and 63 isolates into 15 groups. 29 The reaction-profiles of 88 isolates to a battery of monoclonal antibodies identified 11 distinct patterns; some groups contained isolates from five disparate geographic sites and one geographic site contained representatives of all 11 groups.…”
Section: Discussionmentioning
confidence: 99%
“…T. vaginalis: The organisms used in these studies included clinical isolates of T. vaginalis from patients in Seattle, WA ($13, PHS 2J, $981, Sl190, S1202, and $1220), Brooklyn, NY (B34, kindly supplied by Dr. William McCormack), and Charlottesville, VA (C7, kindly supplied by Dr. Michael Rein). T. vaginaIis isolates $13, PHS 2J, B34, and C7 have been described previously (Krieger et al 1985) and were maintained in liquid nitrogen prior to use in the current studies. $981, Sl190, S1202, and S1220 were fresh clinical isolates from patients attending the Harborview Medical Center Sexually Transmitted Diseases Clinic and the University of Washington Medical Center Prostatitis Clinic.…”
Section: Methodsmentioning
confidence: 99%