Geographic variation in RNAi sensitivity in the migratory locust

Sugahara, Ryohei; Tanaka, Seiji; Jouraku, Akiya; Shiotsuki, Takahiro

doi:10.1016/j.gene.2016.12.028

Cited by 28 publications

(22 citation statements)

References 41 publications

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“…Indeed, some populations of the same species appear to be differentially sensitive to external administration of dsRNA. This was most recently observed in L. migratoria by Sugahara et al (2017). In this research, four different lab strains of the migratory locust were examined, each originating from a geographically isolated location in Japan.…”

Section: Rnai-based Insect Pest Controlmentioning

confidence: 91%

“…Two of the tested lab strains were found to be very sensitive to injection with dsRNA, while the other two appeared to be completely refractory. Even within the same lab strain, different individuals could respond with different degrees of sensitivity (Sugahara et al, 2017). In a comparable research, three phenotypically different field populations of D. virgifera virgifera were given the same dsRNA treatment to see whether they would respond in a similar way.…”

Section: Rnai-based Insect Pest Controlmentioning

confidence: 99%

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RNA Interference in Insects: Protecting Beneficials and Controlling Pests

et al. 2019

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Insects constitute the largest and most diverse group of animals on Earth with an equally diverse virome. The main antiviral immune system of these animals is the post-transcriptional gene-silencing mechanism known as RNA(i) interference. Furthermore, this process can be artificially triggered via delivery of gene-specific double-stranded RNA molecules, leading to specific endogenous gene silencing. This is called RNAi technology and has important applications in several fields. In this paper, we review RNAi mechanisms in insects as well as the potential of RNAi technology to contribute to species-specific insecticidal strategies. Regarding this aspect, we cover the range of strategies considered and investigated so far, as well as their limitations and the most promising approaches to overcome them. Additionally, we discuss patterns of viral infection, specifically persistent and acute insect viral infections. In the latter case, we focus on infections affecting economically relevant species. Within this scope, we review the use of insect-specific viruses as bio-insecticides. Last, we discuss RNAi-based strategies to protect beneficial insects from harmful viral infections and their potential practical application. As a whole, this manuscript stresses the impact of insect viruses and RNAi technology in human life, highlighting clear lines of investigation within an exciting and promising field of research.

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Section: Rnai-based Insect Pest Controlmentioning

confidence: 91%

Section: Rnai-based Insect Pest Controlmentioning

confidence: 99%

RNA Interference in Insects: Protecting Beneficials and Controlling Pests

et al. 2019

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“…(Coleoptera: Brentidae) (Christiaens et al, 2016;Prentice et al, 2017). It can even vary between strains/populations of a particular species as has for example been reported for Locusta migratoria (Orthoptera: Acrididae) (Sugahara et al, 2017) and T. castaneum (Kitzmann et al, 2013;Spit et al, 2017).…”

Section: Hazard Posed By Dsrnamentioning

confidence: 93%

Assessing the Risks of Topically Applied dsRNA-Based Products to Non-target Arthropods

Romeis

Widmer

2020

Front. Plant Sci.

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RNA interference (RNAi) is a powerful technology that offers new opportunities for pest control through silencing of genes that are essential for the survival of arthropod pests. The approach relies on sequence-specificity of applied double-stranded (ds) RNA that can be designed to have a very narrow spectrum of both the target gene product (RNA) as well as the target organism, and thus allowing highly targeted pest control. Successful RNAi has been reported from a number of arthropod species belonging to various orders. Pest control may be achieved by applying dsRNA as foliar sprays. One of the main concerns related to the use of dsRNA is adverse environmental effects particularly on valued non-target species. Arthropods form an important part of the biodiversity in agricultural landscapes and contribute important ecosystem services. Consequently, environmental risk assessment (ERA) for potential impacts that plant protection products may have on valued non-target arthropods is legally required prior to their placement on the market. We describe how problem formulation can be used to set the context and to develop plausible pathways on how the application of dsRNA-based products could harm valued non-target arthropod species, such as those contributing to biological pest control. The current knowledge regarding the exposure to and the hazard posed by dsRNA in spray products for non-target arthropods is reviewed and suggestions are provided on how to select the most suitable test species and to conduct laboratorybased toxicity studies that provide robust, reliable and interpretable results to support the ERA.

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“…Taxonomy cannot be used to reliably predict sensitivity to eRNAi or the latency period between dsRNA uptake and gene silencing. Equivalent eRNAi methods can produce disparate results even when different biotypes (Li et al, 2015a), or subpopulations (Sugahara et al, 2017) of the same species are targeted. Within the Lepidoptera, H. armigera is capable of eliciting a robust eRNAi response (Xiong et al, 2013), but Spodoptera frugiperda is recalcitrant to eRNAi (Ivashuta et al, 2015).…”

Section: Tablementioning

confidence: 99%

“…eRNAi can be achieved via the introduction of dsRNAs via food (Asokan et al, 2014;Coleman et al, 2015;Li et al, 2015b;Sandoval-Mojica & Scharf, 2016) or through topical delivery (Toprak et al, 2013;Whyard et al, 2015). For reasons that are as yet not entirely clear, the capacity of insects to express systemic RNAi and eRNAi varies both within and between species (Baum & Roberts, 2014;Li et al, 2015a;Shukla et al, 2016;Sugahara et al, 2017).…”

mentioning

confidence: 99%

Implementing the sterile insect technique with RNA interference – a review

Darrington

Dalmay

Morrison

et al. 2017

Entomologia Exp Applicata

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We review RNA interference (RNAi) of insect pests and its potential for implementing sterile insect technique (SIT)‐related control. The molecular mechanisms that support RNAi in pest species are reviewed in detail, drawing on literature from a range of species including Drosophila melanogaster Meigen and Homo sapiens L. The underlying genes that enable RNAi are generally conserved across taxa, although variance exists in both their form and function. RNAi represents a plausible, non‐GM system for targeting populations of insects for control purposes, if RNAi effector molecules can be delivered environmentally (eRNAi). We consider studies of eRNAi from across several insect orders and review to what extent taxonomy, genetics, and differing methods of double‐stranded (ds) RNA synthesis and delivery can influence the efficiency of gene knockdown. Several factors, including the secondary structure of the target mRNA and the specific nucleotide sequence of dsRNA effector molecules, can affect the potency of eRNAi. However, taxonomic relationships between insects cannot be used to reliably forecast the efficiency of an eRNAi response. The mechanisms by which insects acquire dsRNA from their environment require further research, but the evidence to date suggests that endocytosis and transport channels both play key roles. Delivery of RNA molecules packaged in intermediary carriers such as bacteria or nanoparticles may facilitate their entry into and through the gut, and enable the evasion of host defence systems, such as toxic pH, that would otherwise attenuate the potential for RNAi.

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Geographic variation in RNAi sensitivity in the migratory locust

Cited by 28 publications

References 41 publications

RNA Interference in Insects: Protecting Beneficials and Controlling Pests

RNA Interference in Insects: Protecting Beneficials and Controlling Pests

Assessing the Risks of Topically Applied dsRNA-Based Products to Non-target Arthropods

Implementing the sterile insect technique with RNA interference – a review

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