Geographical distribution of milky disease bacteria in the eastern United States based on phylogeny

DNA fingerprinting of Paenibacillus popilliae and Paenibacillus lentimorbus using PCR-amplified 16S–23S rDNA intergenic transcribed spacer (ITS) regions

“…1 lanes 3 and 4 show the electrophoretic migration pattern of PCR-amplified ITS DNA for strains KLN 3 (phylogenic group 2) and NRRL B-2309 (phylogenic group 1), respectively. Paenibacillus popilliae has been separated into two phylogenic groupings and P. lentimorbus has been separated into three groupings (Rippere et al, 1998;Dingman, 2008). The nine strains of P. lentimorbus group 2 demonstrated an electrophoretic migration pattern that was identical to the pattern for P. popilliae (data not shown).…”

“…This matching of fingerprints for the different species was expected. Comparison of 16S rDNA sequences has demonstrated such a close relatedness between all P. lentimorbus groups and P. popilliae that species distinction within the milky disease bacteria is not clear (Dingman, 2008). Interestingly, the fingerprint pattern for P. lentimorbus group 3 strains was considerably different from the fingerprints of other milky disease bacteria and clearly identified this group of bacteria.…”