Geographical distributions and host associations of larval parasitoids of frugivorous Drosophilidae in Japan

Mitsui, Hideyuki; Achterberg, Kees van; Nordlander, Göran; Kimura, Masahito T.

doi:10.1080/00222930701504797

Cited by 101 publications

(146 citation statements)

References 15 publications

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“…Populations of A. japonica on the main islands of Japan exhibit highly femalebiased sex ratios (92.7-99.2% females), whereas population sex ratios on the smaller southern islands are not biased (Mitsui et al, 2007). The populations on the main islands are infected with parthenogenesis-inducing Wolbachia, while the populations on the smaller southern islands are not (Kremer et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The strains were kindly provided by M.T. Kimura from cultures derived from field samples, collected along the entire length of Japan, described in Mitsui et al (2007) and Murata et al (2009). Maps of the sampled locations can be found in both papers.…”

Section: A Japonica Strainsmentioning

confidence: 99%

“…The mitochondrial variation among the five thelytokous strains suggests that multiple infection events have occurred and that the Wolbachia infection has spread (partly) via horizontal transmission. The five infected thelytokous strains were collected from the two Japanese main islands, with 1580 km distance between the northernmost location Sapporo and the southernmost location Kagoshima (for maps of the locations see Mitsui et al, 2007 andMurata et al, 2009). The distance between the two main Japanese islands is very small (20 km) and the Wolbachia infection probably spread easily between the two islands.…”

Section: Mtdna Analysismentioning

confidence: 99%

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Occasional males in parthenogenetic populations of Asobara japonica (Hymenoptera: Braconidae): low Wolbachia titer or incomplete coadaptation?

2011

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Wolbachia are endosymbiotic bacteria known to manipulate the reproduction of their hosts. Some populations of the parasitoid wasp Asobara japonica are infected with Wolbachia and reproduce parthenogenetically, while other populations are not infected and reproduce sexually. Wolbachia-infected A. japonica females regularly produce small numbers of male offspring. Because all females in the field are infected and infected females are not capable of sexual reproduction, male production seems to be maladaptive. We investigated why these females nevertheless produce males. We tested three hypotheses: high rearing temperatures could result in higher offspring sex ratios (more males), low Wolbachia titer of the mother could lead to higher offspring sex ratios and/or the Wolbachia infection is of relatively recent origin and not enough time has passed to allow complete coadaptation between Wolbachia and host. In all, 33% of the Wolbachia-infected females produced males and 56% of these males were also infected with Wolbachia. Neither offspring sex ratio nor male infection frequency was significantly affected by rearing temperature or Wolbachia concentration of the mother. The mitochondrial DNA sequence of one of the uninfected populations was identical to that of two of the infected populations. Therefore, the initial Wolbachia infection of A. japonica must have occurred recently. Mitochondrial sequence variation among the infected populations suggests that the spread of Wolbachia through the host populations involved horizontal transmission. We conclude that the occasional male production by Wolbachia-infected females is most likely a maladaptive side effect of incomplete coevolution between symbiont and host in this relatively young infection.

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Section: Introductionmentioning

confidence: 99%

Section: A Japonica Strainsmentioning

confidence: 99%

Section: Mtdna Analysismentioning

confidence: 99%

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Occasional males in parthenogenetic populations of Asobara japonica (Hymenoptera: Braconidae): low Wolbachia titer or incomplete coadaptation?

2011

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“…Biological system Asobara japonica (Hymenoptera:Braconidae:Alysiinae) is a solitary endoparasitoid laying its eggs into the first or second instar larvae of Diptera and especially Drosophila species (Mitsui et al, 2007;Ideo et al, 2008). Wasps were trapped in the field using banana and natural fruits found in some localities (see Mitsui et al 2007).…”

Section: Methodsmentioning

confidence: 99%

“…A. rufescens is partially infected by a strain closely related to the CI-inducing strain wAtab1 (Dedeine et al, 2005). In a recent survey of larval parasitoids of frugivorous Drosophilidae in Japan, Mitsui et al (2007) established the geographical distribution of A. japonica. They showed that field sex ratios are typical of arrhenotokous reproduction in three populations on subtropical islands, whereas those of all five populations sampled on the main (northern) islands are highly female biased.…”

Section: Introductionmentioning

confidence: 99%

A new case of Wolbachia dependence in the genus Asobara: evidence for parthenogenesis induction in Asobara japonica

et al. 2009

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Wolbachia is a maternally inherited bacterium that is widely distributed among arthropods, in which it manipulates the reproduction of its hosts. Although generally facultative for its hosts, Wolbachia has recently become obligatory in Asobara tabida (Hymenoptera: Braconidae) in which it is required for the completion of oogenesis. Here, we describe a new Wolbachia strain (wAjap) that is associated with the genus Asobara and infects Asobara japonica. wAjap was detected in all female-biased populations of A. japonica found in the main islands of Japan, but not in the arrhenotokous populations from the southern islands. Using phylogenetic analyses based on multi-locus sequence typing (MLST), we show that this strain is closely related to wAtab3 (the strain required for oogenesis in A. tabida), even though they differ on Wolbachia surface protein (WSP) and WO phage sequences. Using antibiotic treatments, we show that cured thelytokous females are not dependent on Wolbachia for oogenesis. However, they produced only sons, showing that wAjap induces thelytokous parthenogenesis. Analyses of mating behavior and offspring production of individuals from Wolbachia-infected populations showed that while males were still sexually functional, females no longer attract males, making Wolbachia an obligate partner for daughter production in thelytokous populations. The fact that Wolbachia has become independently obligatory in two species of the same genus tends to show that dependence evolution can be common and swift, although no clear benefit for the parasitoid can be attributed to this dependence. Although dependence should lead to co-divergence between Wolbachia and its hosts, the very few cases of co-speciation observed in host-Wolbachia associations question the stability of these obligatory associations.

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VENOM COMPONENTS OF Asobara japonica IMPAIR CELLULAR IMMUNE RESPONSES OF HOST Drosophila melanogaster

Furihata

Matsumoto

Kimura

et al. 2013

Arch Insect Biochem Physiol

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The endoparasitoid wasp Asobara japonica has highly poisonous venom: the host Drosophila larvae are killed by envenomation at a dose that is naturally injected by the female wasp at parasitism. This insecticidal venom is neutralized, however, because A. japonica introduces lateral oviduct components soon after venom injection at oviposition. Although the venom and lateral oviduct components of this parasitoid have been partially characterized, how the venom components favor successful development of wasp eggs and larvae in the host remains ambiguous. Here, we demonstrated that A. japonica venom did not affect host humoral immune responses, determined as expression of antimicrobial peptide (AMP) genes, but significantly diminished two cellular responses, spreading and phagocytosis, by host hemocytes. Moreover, venom components drastically elevated a serine protease-like activity 4 h after its injection. The lateral oviduct components did not negate the detrimental effects of the venom on host cellular immunities, but significantly reduced the venom-induced elevation of protease activity. Both active factors in venom and lateral oviduct components were roughly characterized as heat-labile substances with a molecular mass of at least 10 kDa. Finally, venom of A. japonica, with a wide host range, was found to be much more toxic than that of Asobara rossica, which has a limited host range. These results reveal that A. japonica venom toxicity allows exploitation of a broader range of host insects because it is essential to overcome cellular immune responses of the host for successful parasitism.

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Geographical distributions and host associations of larval parasitoids of frugivorous Drosophilidae in Japan

Cited by 101 publications

References 15 publications

Occasional males in parthenogenetic populations of Asobara japonica (Hymenoptera: Braconidae): low Wolbachia titer or incomplete coadaptation?

Occasional males in parthenogenetic populations of Asobara japonica (Hymenoptera: Braconidae): low Wolbachia titer or incomplete coadaptation?

A new case of Wolbachia dependence in the genus Asobara: evidence for parthenogenesis induction in Asobara japonica

VENOM COMPONENTS OF Asobara japonica IMPAIR CELLULAR IMMUNE RESPONSES OF HOST Drosophila melanogaster

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