Geographical variation of gene diversity of Pinus pinaster Ait. in the Iberian Peninsula

González‐Martínez, Santiago C.; Salvador, L.; Agúndez, D.; Alı́a, Ricardo; Gil, Lorenzo

doi:10.1007/978-94-015-9839-2_14

Cited by 20 publications

(17 citation statements)

References 20 publications

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“…The selection of a low number of Iberian populations is based on previous studies. In fact, the four Spanish populations are typical locations of the four main groups of populations detected in Spain with allozymes by Salvador et al [24] and González-Martínez et al [11]: North West, East and South East (which is divided in two subgroups, both represented in the present study).…”

Section: Geographical Genetic Differentiation Of P Pinastersupporting

confidence: 63%

“…Several range-wide genetic diversity surveys have been carried out using terpenes, isozymes, denaturated proteins and chloroplast microsatellites (Baradat and Marpeau-Bezard [4]; Bahrman et al [3]; Petit et al [19]; Vendramin et al [25]). Recent studies have been undertaken at a regional level using isozymes, AFLP markers (Amplified Fragment Length Polymorphisms), nuclear and chloroplast microsatellite markers (Salvador et al [24]; González-Martínez et al [11]; Mariette et al [18]; Ribeiro et al [21]). A test based on chloroplast microsatellites has very recently been developed in order to determine the putative origin of P. pinaster stands in the Aquitaine region of France (Ribeiro et al [22]).…”

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confidence: 99%

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What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies?

et al. 2002

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-Maritime pine (Pinus pinaster Ait.) is the first conifer used for reforestation in France and now covers 2.4 million ha of the Iberian Peninsula. In order to preserve the genetic resources of this economically and ecologically important species prior knowledge of the distribution of genetic diversity is needed. In this paper, a genetic diversity study was performed using nuclear simple sequence repeats (SSRs or microsatellites). Classical parameters of diversity (allelic richness and heterozygosity) and differentiation were estimated for 47 populations of P. pinaster. Most of the populations (40) were collected in France, six populations were also collected in the Iberian Peninsula and one Moroccan population was also included in the study. The population genetic parameters indicated that some populations should be a focus of conservation efforts (higher level of diversity, higher allelic richness and presence of rare alleles). A diagnostic test for sample origin was developed to distinguish Corsican from Landes populations.Pinus pinaster / nuclear microsatellites / genetic diversity / conservation / provenance identification Résumé -Que nous indiquent les microsatellites nucléaires sur la conservation des ressources génétiques du pin maritime et sur les stratégies de certification de provenances ? Le pin maritime (Pinus pinaster Ait.) est le premier conifère utilisé pour le reboisement en France et couvre environ 2,4 millions d'hectares dans la péninsule Ibérique. Dans le but de conserver les ressources génétiques de cette espèce, importante du point de vue économique et écologique, une connaissance préalable de la distribution de sa diversité génétique est nécessaire. Dans ce papier, une étude de diversité génétique a été menée en utilisant des marqueurs microsatellites. Les paramètres classiques de diversité (richesse allélique et hétérozygotie) et de différenciation ont été calculés au sein de 47 populations. La plupart des populations (40) ont été échantillonnées en France, six populations ont été choisies dans la Péninsule Ibérique et une population marocaine a également été incluse dans l'analyse. Les résul-tats de génétique des populations montrent que certaines populations pourraient être intéressantes pour la conservation des ressources génétiques de l'espèce (niveau d'hétérozygotie ou de richesse allélique plus élevée que les autres populations, présence d'allèles rares). Nous avons montré que les résultats de cette analyse fournissent un test diagnostic pour distinguer les populations d'origine landaise des populations d'origine corse.Pinus pinaster / microsatellites nucléaires / diversité génétique / conservation / certification de provenance

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Section: Geographical Genetic Differentiation Of P Pinastersupporting

confidence: 63%

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confidence: 99%

What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies?

et al. 2002

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“…An alternative explanation is derived from latitudinal environmental variation. Whereas allozyme variation along the cline is explained due to historical events related to migration pathways (Salvador et al, 2000;González-Martínez et al, 2001), the proportional genetic differentiation in survival could be explained as the selective pressure of parameters with latitudinal variation and not only as a result of non-selective forces. In the populations studied, mean temperature and precipitation range from 9.7°C to 14.0°C and from 550 mm to 985 mm, respectively.…”

Section: Discussionmentioning

confidence: 99%

Population genetic structure in a Mediterranean pine (Pinus pinaster Ait.): a comparison of allozyme markers and quantitative traits

González‐Martínez¹,

Alı́a²,

Gil³

2002

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F-statistics were employed to analyse quantitative and allozyme variation among 19 native populations of maritime pine (Pinus pinaster Ait.). Fourteen polymorphic allozyme loci were used to provide an empirical basis for constructing a null hypothesis to test natural selection as a determinant of quantitative evolution in stem form, total height growth and survival at 30 years old. Hidden biases, that may result in a difference between quantitative (Q ST ) and allozyme (F ST ) differentiation which are not because of the action of natural selection, were avoided by comparing pairs of populations using linear models. All quantitative traits showed higher differentiation than allozymes. The highest divergence was

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“…Amplifications were carried out using an Uno-Thermoblock thermal cycler. The amplification conditions followed the protocol explained by González-Martínez et al (2001). After the amplification, 1 µL of PCR product (for FRPP91 and ITPH4516) and 0.5 µL (FRPP94) were mixed with 24 µL of deionised formamide (SLS, Sample Loading Solution), with 0.5 µL of CEQ Well Red fluorescent dye and one drop of mineral oil for fragment analysis.…”

Section: Study Site and Plant Materialsmentioning

confidence: 99%

Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

et al. 2008

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Abstract• Relatedness among parents, variation in clonal fertility and background pollination deviate the realized genetic gain and the gene diversity of open pollinated seed orchard from expectation, in particular in wind pollinated species such as Pinus pinaster Aiton.• This work investigates the genetic variation, the mating system and the pollen contamination in a P. pinaster clonal seed orchard (CSO), by screening the 60 clones from the CSO and the seeds collected from 21 mother-trees with three nuclear microsatellites.• The expected diversity was similar, but the observed heterozygosity decreased 20% in the progenies compared with the parental trees. The outcrossing rate was 90.1%, the biparental inbreeding 21.7% computed through a multilocus approach, and the observed selfing 3.9%. The observed gene flow from outside the CSO was 52.4%.• From the results we concluded that the observed gene flow and the biparental inbreeding were high, and care should be taken in the implementation and management of future CSO, in particular clones should be checked for relatedness and the ramet number could be directly proportional to their breeding value. • L'apparentement entre parents, la variation de fertilité entre clones et la pollution pollinique font dévier le gain génétique réalisé et la diversité génétique des valeurs attendues en verger à graines de clones à pollinisation libre, en particulier pour une espèce à pollinisation anémophile comme Pinus pinaster Aiton. Mots-clés• Ce travail étudie la variation génétique, le système de reproduction et la contamination du pollen d'un verger à graines de clones (VGC) de P. pinaster, en génotypant les 60 clones du VGC et les graines récoltées sur 21 arbres-mères pour trois marqueurs microsatellites nucléaires.• La diversité attendue s'est révélée similaire mais l'hétérozygotie observée a diminué de 20 % chez les descendants par rapport aux parents. Le taux d'allofécondation était de 90,1 %, la consanguinité biparentale calculée grâce à une approche multilocus était de 21,7 % et le taux d'autofécondation observé était de 52,4 %.• D'après ces résultats, nous avons conclu que les flux de gènes observés et la consanguinité biparentale étaient élevés et que des précautions devaient être prises dans la mise en place et la gestion de futurs VGC, l'apparentement entre clones devrait en particulier être vérifié et le nombre de copies d'un individu pourrait être directement proportionnel à sa valeur génétique.

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Geographical variation of gene diversity of Pinus pinaster Ait. in the Iberian Peninsula

Cited by 20 publications

References 20 publications

What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies?

What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies?

Population genetic structure in a Mediterranean pine (Pinus pinaster Ait.): a comparison of allozyme markers and quantitative traits

Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard

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