[1] The oceanic mass balance of calcium (Ca) is defined by a balance between the inputs (rivers and hydrothermal) and outputs (bulk carbonate) of Ca. Large rivers were analyzed for Ca isotope ratios ( 44 Ca/ 42 Ca, expressed as d 44 42 Ca) to investigate the source and cycling of riverine Ca, and to add an isotopic mass balance constraint to the oceanic budget of Ca. The new data account for approximately one-third of the total Ca supplied to the oceans by rivers. Inter-sample and seasonal variability was assessed by analyzing more than one sample for many rivers. The range in the d Ca value of bulk carbonate and the riverine input at the current level of uncertainty. The input and output fluxes could be imbalanced over much shorter time-scales (such as glacial-interglacial cycles), in which case the ocean-carbonate system will not yet have responded, because of the long residence time of Ca. The maximum current flux imbalance of 15% would be sufficient to account for the total variations in Ca concentration over the Tertiary. Such an interpretation is not unique, but is the simplest interpretation given the similarity between the input and output isotopic compositions, and rules out hypotheses of extreme imbalance in the recent global biogeochemical cycle of Ca.Citation: Tipper, E. T., J. Gaillardet, A. Galy, P. Louvat, M. J. Bickle, and F. Capmas (2010), Calcium isotope ratios in the world's largest rivers: A constraint on the maximum imbalance of oceanic calcium fluxes, Global Biogeochem. Cycles, 24, GB3019,