Geothermomicrobium terrae gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae

Abstract

Geothermomicrobium terrae gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae Strains YIM 77562 T and YIM 77580, two novel Gram-staining-positive, filamentous bacterial isolates, were recovered from the Rehai geothermal field, Tengchong, Yunnan province, southwest China. Good growth was observed at 50-55 6C and pH 7.0. Aerial mycelium was absent on all media tested. Substrate mycelium was well-developed, long and moderately flexuous, and formed abundant, single, warty, ornamented endospores. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains indicated that they belong to the family Thermoactinomycetaceae. Similarity levels between the 16S rRNA gene sequences of the two strains and those of type strains of members of the Thermoactinomycetaceae were 88.33-93.24 %; the highest sequence similarity was with Hazenella coriacea DSM 45707 T . In both strains, the predominant menaquinone was MK-7, the diagnostic diamino acid was mesodiaminopimelic acid and the major cellular fatty acids were iso-C 14 : 0 , iso-C 15 : 0 and iso-C 16 : 0 . The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, unidentified polar lipids and unidentified phospholipids. The genomic DNA G+C contents of strains YIM 77562 T and YIM 77580 were 45.5 and 44.2 mol%, respectively. DNA-DNA relatedness data suggest that the two isolates represent a single species. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that the two strains represent a single novel species in a new genus, Geothermomicrobium terrae gen. nov., sp. nov. The type strain of Geothermomicrobium terrae is YIM 77562 T (5CCTCC AA 2011022 T 5JCM 18057 T ).

Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients

“…Desmospora, Marinithermofilum, Hazenella) or environmental sources (e.g. Mechercharimyces, Lihuaxuella, Geothermomicrobium and Risungbinella) (Matsuo et al 2006;Yassin et al 2009;Yu et al 2012;Buss et al 2013;Zhou et al 2014;Kim et al 2015;Zhang et al 2015). Salient properties of the family Thermoactinomycetaceae include thermotolerant growth up to 60 °C, Gram positivity, non-acid fastness and formation of single spores that may be sessile or on simple sporophores with the structure and properties of endospores.…”

Risungbinella pyongyangensis gen. nov., sp. nov., a mesophilic member of the family Thermoactinomycetaceae isolated from an agricultural soil sample

“…Tsilinsky (1899) proposed a new genus Thermoactinomyces with the capacity to grow at high temperatures (50–55 °C), which was the first described member of the family Thermoactinomycetaceae (Matsuo et al , 2006). At the time of writing, on the basis of 16S rRNA gene sequence analysis, as well as chemotaxonomic and physiological characterization, the family Thermoactinomycetaceae accommodates 14 genera: Thermoactinomyces (Tsilinsky, 1899), Laceyella , Thermoflavimicrobium , Seinonella (Yoon et al , 2005), Planifilum (Hatayama et al , 2005), Mechercharimyces (Matsuo et al , 2006), Shimazuella (Park et al , 2007), Desmospora (Yassin et al , 2009), Kroppenstedtia (von Jan et al , 2011), Marininema (Li et al , 2012), Melghirimyces (Addou et al , 2012), Lihuaxuella (Yu et al , 2012), Hazenella (Buss et al , 2013), Polycladomyces (Tsubouchi et al , 2013) and Geothermomicrobium (Zhou et al , 2014). All known members of the family Thermoactinomycetaceae are Gram-positive, aerobic and thermophilic, with the exception of three mesophilic species, Seinonella peptonophila (Nonomura & Ohara, 1971; Yoon et al , 2005; basonym Thermoactinomyces peptonophilus ), Mechercharimyces mesophilus (Matsuo et al , 2006) and Shimazuella kribbensis (Park et al , 2007).…”

“… * Data taken from: a , Park et al (2007); b , Zhou et al (2014); c , Yu et al (2012). † DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIM, phosphatidylinositol mannosides; PME, phosphatidylmethylethanolamine; AL, unknown aminolipid; APL, unknown aminophospholipid; PL, unknown phospholipid; L, unknown polar lipid; ‡ Ara, Arabinose; Gal, galactose; Glc, glucose; Man, mannose; Rha, rhamnose; Rib, ribose; Xyl, xylose. …”

Marinithermofilum abyssi gen. nov., sp. nov. and Desmospora profundinema sp. nov., isolated from a deep-sea sediment, and emended description of the genus Desmospora Yassin et al. 2009

“…At the time of writing, 16 genera and 29 recognized species have been described in the family Thermoactinomycetaceae . The genera Shimazuella (Park et al , 2007), Desmospora (Yassin et al , 2009), Kroppenstedtia (von Jan et al , 2011), Melghirimyces (Addou et al , 2012), Lihuaxuella (Yu et al , 2012), Marininema (Li et al , 2012), Polycladomyces (Tsubouchi et al , 2013), Hazenella (Buss et al , 2013), Geothermomicrobium (Zhou et al , 2014) and Salinithrix (Zarparvar et al , 2014) have been found in succession and assigned to this family. Strains of this family have been isolated from various environmental samples, such as marine sediments, salt lakes, sugar cane, mushroom compost and clinical samples.…”