Gerbera micropropagation

Cardoso, Jean Carlos; Silva, Jaime A. Teixeira da

doi:10.1016/j.biotechadv.2013.05.008

Cited by 40 publications

(27 citation statements)

References 75 publications

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“…In vitro culture has been of great relevance for the genetic improvement of this crop, including the development of doubled haploids to obtain pure lines [3], or the development of the first commercial transgenic Bt eggplant [4]. However, as it occurs in other crops such as onion [5][6][7] or gerbera [8], available protocols to regenerate eggplants are mostly inefficient or highly dependent on the genotype [9][10][11]. Thus, more efficient and reproducible protocols suitable to a wide range of genotypes are needed to circumvent the current drawbacks for in vitro regeneration in eggplant, mainly those related to the strong effect that the genotype has on regeneration efficiency, and even more if we take into account that globally over 6600 eggplant accessions are currently available in the germplasm Genesys database [12].…”

Section: Introductionmentioning

confidence: 99%

A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

et al. 2020

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Background: Efficient organogenesis induction in eggplant (Solanum melongena L.) is required for multiple in vitro culture applications. In this work, we aimed at developing a universal protocol for efficient in vitro regeneration of eggplant mainly based on the use of zeatin riboside (ZR). We evaluated the effect of seven combinations of ZR with indoleacetic acid (IAA) for organogenic regeneration in five genetically diverse S. melongena and one S. insanum L. accessions using two photoperiod conditions. In addition, the effect of six different concentrations of indolebutyric acid (IBA) in order to promote rooting was assessed to facilitate subsequent acclimatization of plants. The ploidy level of regenerated plants was studied. Results: In a first experiment with accessions MEL1 and MEL3, significant (p < 0.05) differences were observed for the four factors evaluated for organogenesis from cotyledon, hypocotyl and leaf explants, with the best results obtained (9 and 11 shoots for MEL1 and MEL3, respectively) using cotyledon tissue, 16 h light / 8 h dark photoperiod conditions, and medium E6 (2 mg/L of ZR and 0 mg/L of IAA). The best combination of conditions was tested in the other four accessions and confirmed its high regeneration efficiency per explant when using both cotyledon and hypocotyl tissues. The best rooting media was R2 (1 mg/L IBA). The analysis of ploidy level revealed that between 25 and 50% of the regenerated plantlets were tetraploid. Conclusions: An efficient protocol for organogenesis of both cultivated and wild accessions of eggplant, based on the use of ZR, is proposed. The universal protocol developed may be useful for fostering in vitro culture applications in eggplant requiring regeneration of plants and, in addition, allows developing tetraploid plants without the need of antimitotic chemicals.

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Section: Introductionmentioning

confidence: 99%

A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

et al. 2020

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“…(Sujatha et al, 2002). Gerbera belongs to the family Asteraceae (Cardoso and Silva, 2013). Gerbera comprises 45 species, native to tropical Asia and Africa (Keditsu R. 2013).…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Gerbera Irrigated With Treated Petroleum Refinery Wastewater

Soliman

El-Azab

N.³

2017

Journal of Environmental Science

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A detailed evaluation for the use of treated petroleum refinery wastewater in irrigation of Gerbera plants, vegetative growth, production and chemical compounds of Gerbera jamesonii were determined. The experiment was conducted for two successive seasons of 2014 and 2015 at the Floriculture Nursery under full sunlight conditions at Horticulture Research Institute, Ornamental Plants and Landscape Gardining Research Department, Egypt using clay loam soil and 30 cm pots. The mean plant height in harvest was 32.33 +1.86 and 25.67+ 2.18 cm for irrigation with treated wastewater at the first and second seasons respectively. Mean number of roots /plant in harvest was 42.33 +5.5 and 42.67+ 2.03 for irrigation with treated wastewater at the first and second seasons respectively. Mean flowers number / plant in harvest were 6.42 +1.1 and 6.81+ 1.3 for irrigation with treated wastewater at the first and second seasons respectively. Mean flower diameters in harvest were 7.44 +1.14 and 7.1+ 1.18 cm for irrigation with treated wastewater at the first and second seasons respectively. The mean numbers of leaves / plant in harvest were 8.33 +0.37 and 6.0+ 1.0 for irrigation with treated wastewater at the first and second seasons respectively.

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“…Gerbera (Gerbera jamesonii) is an herbaceous ornamental plant belonging to the family Asteraceae (CARDOSO and SILVA, 2013). Leaves are arranged in a rosette shape and the petiole of some of them evolves floral buds that develop peduncles with terminal inflorescence in the head (INFOAGRO, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nutrition and growth of potted gerbera according to mineral and organic fertilizer

Santos

Ludwig

Costa

et al. 2015

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In order to meet the growing market of gerbera, it is necessary to develop studies that maximize its production, especially using organic fertilizer. In order to assess the nutrition and growth of potted gerbera conducted with mineral and organic fertilization, an experiment in a greenhouse was done, located at Western Paraná State University, Brazil. The experimental design was made in randomized blocks, with four replications and five treatments. The treatments were defined according to the source of fertilization, mineral (NPK) or organic. The organic fertilization were obtained by diluting in water four organic compost of poultry slaughter waste, obtained from in the composting process, in order to adjust electrical conductivity. The solution of the compost was used as organic fertigation, making the organic treatments. The liquid organic fertilizer, as well as irrigation of mineral treatment, was performed manually once a day. At the end of vegetative and reproductive periods, the levels of N, P, K, Ca, Mg and Fe were quantified in the plant tissue. At the same time, biometric parameters were assessed (number of leaves, plant diameter, leaf area, dry matter of aerial parts, number of heads, inflorescence dry matter, stem height, head diameter and diameter stem). The liquid organic fertilizers, obtained by composting procession of poultry slaughter waste, can be used as alternative source for potted gerbera nutrition, since they provide better or higher culture growth than the mineral fertilizer.

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Gerbera micropropagation

Cited by 40 publications

References 75 publications

A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

Cultivation of Gerbera Irrigated With Treated Petroleum Refinery Wastewater

Nutrition and growth of potted gerbera according to mineral and organic fertilizer

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