Germin-like proteins (GLPs) in cereal genomes: gene clustering and dynamic roles in plant defence

Breen, James; Bellgard, M.

doi:10.1007/s10142-010-0184-1

Cited by 70 publications

(58 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A large number of GLP genes have been discovered by genome sequencing or by expressed sequence tags (ESTs) in higher plants like Arabidopsis (Carter et al 1998;Membré et al 1997), barley (Druka et al 2002;Wu et al 2000), rice (Carrillo et al 2009;Membré and Bernier 1998), and peanut (Chen et al 2011). The closely related GLPs from different cereal genomes available in a public database were compared in a review by Breen and Bellgard (2010). The review also discussed the importance of GLP family members in plant defense due to the overlapping of some of the GLP cluster with the disease-resistance quantitative trait loci (QTL).…”

Section: Glp Gene Family Membersmentioning

confidence: 99%

Versatility of germin-like proteins in their sequences, expressions, and functions

Barman

Banerjee

2015

Funct Integr Genomics

View full text Add to dashboard Cite

Germin-like proteins (GLPs) are evolutionary conserved ubiquitous plant glycoproteins belonging to the cupin superfamily. A large number of GLP family members have been identified from different higher and lower plant species, and those have been classified into different subfamilies. Although three histidine residues (H) and one glutamate residue (E) in germin box B and C were conserved among all the GLP subfamily members, how the sequences of one subfamily member differ from the other is unclear. Progress in the field of genomics, transcriptomics, and proteomics has made it possible to understand the variation at gene level among different GLP members from diverse genera and also their biological significances. GLPs from different plant species were found to have various enzymatic properties including oxalate oxidase (OxO), superoxide dismutase (SOD), ADP glucose pyrophosphatase/phosphodiesterase (AGPPase), and polyphenol oxidase (PPO) activities. 'Omics' study demonstrated the expression as well as involvement of GLP family members in almost every part of higher plants as well as in lower plants. Additionally, GLPs from different species were reported to be involved in biotic as well as abiotic stresses and also in the growth and development. This review describes the present research status of GLPs from different plant species, their expressions, and functional significances. Sequence variation was detected among GLP subfamily members at the amino acid level, and based on the sequence variation and phylogenetic analyses, two new GLP subfamilies have been proposed in this review.

show abstract

Section: Glp Gene Family Membersmentioning

confidence: 99%

Versatility of germin-like proteins in their sequences, expressions, and functions

Barman

Banerjee

2015

Funct Integr Genomics

View full text Add to dashboard Cite

show abstract

“…Manosalva et al (2009) reported that a GLP gene member located on chromosome 8 in rice provides resistance against rice blast and sheath blight disease. GLPs were studied by comparing the gene clustering in cereal genomes with rice, the model plant among grass species (Breen and Bellgard 2010). It was shown that the overlapping of gene clusters with specific QTLs associated with disease resistance contributes against pathogens.…”

Section: Introductionmentioning

confidence: 99%

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

Munir

Hayashi

Batley

et al. 2015

Funct Integr Genomics

View full text Add to dashboard Cite

Controlled transgene expression via a promoter is particularly triggered in response to pathogen infiltration. This is significant for eliciting disease-resistant features in crops through genetic engineering. The germins and germin-like proteins (GLPs) are known to be associated with plant and developmental stages. The 1107-bp Oryza sativa root GLP2 (OsRGLP2) gene promoter fused to a β-glucuronidase (GUS) reporter gene was transformed into potato plants through an Agrobacterium-mediated transformation. The OsRGLP2 promoter was activated in response to Fusarium solani (Mart.) Sacc. and Alternaria solani Sorauer. Quantitative real-time PCR results revealed 4-5-fold increase in promoter activity every 24 h following infection. There was a 15-fold increase in OsRGLP2 promoter activity after 72 h of F. solani (Mart.) Sacc. treatment and a 12-fold increase observed with A. solani Sorauer. Our results confirmed that the OsRGLP2 promoter activity was enhanced under fungal stress. Furthermore, a hyperaccumulation of H2O2 in transgenic plants is a clear signal for the involvement of OsRGLP2 promoter region in the activation of specific genes in the potato genome involved in H2O2-mediated defense response. The OsRGLP2 promoter evidently harbors copies of GT-I and Dof transcription factors (AAAG) that act in response to elicitors generated in the wake of pathogen infection.

show abstract

“…In contrast to a true germin subfamily, both GLP1 and GLP2 subfamilies are limited to proteins of SOD action, while GLP3 is involved in phosphodiesterase activities. However, recently more subdivisions were suggested (Breen and Bellgard, 2010). A key feature of the germin and GLP subdivisions was the conservation of a motif derived from that of the cupin superfamily Thornburg, 1999, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Most of them share biochemical features such as sucrose-binding, globulins, and seed storage proteins, though they differ in their enzyme activities and tissue specificities (Kim et al, 2004). GLPs have been found in seeds, flowers, embryos, cotyledons, roots, stems, and leaves and were shown to be involved in several essential processes including apoptosis, defense, photoperiodic oscillation, development, and osmotic regulation (Mahmut, 2000;Breen and Bellgard, 2010). TATA box-binding protein (TBP) is a general eukaryotic transcription factor that is required by all three eukaryotic RNA polymerases for correct initiation of transcription of messenger, transfer, ribosomal, and small nuclear RNAs.…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of regulatory elements in the germin and germin-like proteins family promoters in rice

Ali¹,

Mahmood²

2015

Turk J Bot

View full text Add to dashboard Cite

IntroductionGermins and germin-like proteins (GLPs) belong to the cupin superfamily of proteins, identified in several plants, including monocot, dicot, gymnosperms, Physarum polycephalum, and myxomycete (slime mold), after initial discovery of a protein marker germin in the germination of wheat seeds. Germin relatives have also been recognized in animals, prokaryotes, and fern spores (Lu et al., 2010).Germins and GLPs present major problems in classification due to their high sequence conservation in multiple plant species (Dunwell and Gane, 1998). A strong classification system is established by structural characteristics of these proteins due to the clustering of proteins with conserved functions (Agarwal et al., 2009). The proteins that have oxalate oxidase (OXO) enzymes activity, exclusively found within cereal plant species, are placed in a well-conserved homogeneous group called true germins (Carrillo et al., 2009;Davidson et al., 2009), while the germin-motif comprising proteins with an average of 50% amino acid sequence identity that either do not have OXO action or have not yet been allotted an enzymatic function are placed in a heterogeneous group called germinlike protein (Dunwell et al., 2008;Breen and Bellgard, 2010).To date, major research on germins and GLPs has been conducted on cereal plants, especially wheat, maize, barley, and rice. On the basis of various enzymatic activities, six germin subfamilies (GER1-6) have been described. GER1 (true germin proteins) has been shown to possess OXO activity, while GER2 has been shown to possess superoxide dismutase (SOD) action (Banerjee and Maiti, 2010). It has also been shown that the GER1 subfamily is important for early plant development and germination in plants (Federico et al., 2006). The GLPs with mostly unknown function in plant genomes have been classified into subfamilies Thornburg, 1999, 2000). In contrast to a true germin subfamily, both GLP1 and GLP2 subfamilies are limited to proteins of SOD action, while GLP3 is involved in phosphodiesterase activities. However, recently more subdivisions were suggested (Breen and Bellgard, 2010). A key feature of the germin and GLP subdivisions was the conservation of a motif derived from that of the cupin superfamily (Carter and Thornburg,

show abstract

Germin-like proteins (GLPs) in cereal genomes: gene clustering and dynamic roles in plant defence

Cited by 70 publications

References 109 publications

Versatility of germin-like proteins in their sequences, expressions, and functions

Versatility of germin-like proteins in their sequences, expressions, and functions

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

Identification and analysis of regulatory elements in the germin and germin-like proteins family promoters in rice

Contact Info

Product

Resources

About