Germinação in vitro e organogênese direta em explantes hipocotiledonares com polaridade invertida de Psychotria ipecacuanha (Brot.) Stokes

Silva, Maurecilne L. da; Azevedo, Aristéa Alves; Silva, Gizelly M. da; Carvalho, Ilio Fealho de; Rossi, Ana Aparecida Bandini; Otoni, Wagner Campos

doi:10.32712/2446-4775.2013.189

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Another study by Soares and Miranda (2016) confirms that the best dose of IBA in citrus was 2.0 and 1.0 mg L -1 of IBA. It is also worth mentioning the studies by Silva et al (2013) who carried out studies with Psychotria ipecacuanha and showed that the best IBA concentration for root formation was 1.5 mg L -1 IBA. Furthermore, according to studies by Pedrotti (2001), when using auxins in the culture medium, high doses can influence the formation of calluses.…”

Section: Resultsmentioning

confidence: 96%

Physiological responses of 6-benzylaminopurine on multiplication and indolebutyric acid on in vitro rooting of Eucalyptus dunnii Maidenizamento in vitro de Eucalyptus dunnii Maiden

Gunther,

Fermino Junior

2021

SciNat

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Eucalyptus dunnii Maiden is a wood species of wide interest and use in southern Brazil due to its adaptation to the cold climate. Low seed production favors use of plant tissue culture techniques for propagation. The objective of this work was to establish in vitro multiplication of E. dunnii, using micropropagation technique via direct organogenesis, evaluating different concentrations of 6benzylaminopurine (BAP) in multiplication of the stem and cotyledon nodal segments, in addition to effect of different concentrations of indolebutyric acid (IBA) at rooting. After 30 days of culture, stem nodal segments had an increase in the percentage of shoots when subjected to low concentrations of BAP, while for the cotyledon nodal segments the absence of growth regulator and the use of a concentration of 2.0 mg L -1 of BAP showed the highest percentage of shoot formation. The increase in IBA concentration promoted increase in number of adventitious roots. In vitro propagation of E. dunnii using BAP and IBAare a viable protocol.

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Section: Resultsmentioning

confidence: 96%

Physiological responses of 6-benzylaminopurine on multiplication and indolebutyric acid on in vitro rooting of Eucalyptus dunnii Maidenizamento in vitro de Eucalyptus dunnii Maiden

Gunther,

Fermino Junior

2021

SciNat

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“…Given the commercial value of this plant, some initiatives for cloning and micropropagation in the laboratory have already been carried out, but there is still a need to optimize these protocols to test different dosages of growth regulators to increase seedling productivity, ensuring the economic viability of this cultivation technique (12) . Due to the high economic and pharmacological value of the species and associated market demand combined with the extinction risk and the difficulty of the conventional cultivation, the objective of this study was to evaluate the effect of different dosages of the regulator (IAA) on the in vitro development of the plant.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid

Silva

Pinheiro

Assunção

et al. 2018

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Psychotria ipecacuanha, é uma planta medicinal nativa da América com a maior área de ocorrência no Estado de Mato Grosso - Brasil, críticamente ameaçada devido à sobreexploração das populações naturais. Devido à dificuldades na propagação convencional, o objetivo deste estudo foi a avaliação do efeito de diferentes concentrações do regulador de crescimento ácido indolacético (AIA) na propagação in vitro de P. ipecacuanha. Segmentos nodais foram cultivados em meio de Murashige e Skoog (MS) sem reguladores de crescimento (controle) e suplementado com quatro concentrações (0,05; 0,5; 1,5 e 2,0 mg.L-1) de AIA, em meios semi-sólidos. Após 60 dias de cultivo, segmentos nodais (n=30) cultivados em meio de Murashige & Skoog (MS) suplementado com 0,05 mg.L-1 de AIA produziram, em média, 4,56 segmentos nodais por explante, cujas plântulas foram aclimatizadas com sucesso, sem exibição de qualquer anomalia morfológica ou variação.

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Germinação in vitro e organogênese direta em explantes hipocotiledonares com polaridade invertida de Psychotria ipecacuanha (Brot.) Stokes

Cited by 2 publications

References 16 publications

Physiological responses of 6-benzylaminopurine on multiplication and indolebutyric acid on in vitro rooting of Eucalyptus dunnii Maidenizamento in vitro de Eucalyptus dunnii Maiden

Physiological responses of 6-benzylaminopurine on multiplication and indolebutyric acid on in vitro rooting of Eucalyptus dunnii Maidenizamento in vitro de Eucalyptus dunnii Maiden

In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid

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