Germination and pollen tube formation

Cresti, Mauro; Tiezzi, Antonio

doi:10.1016/b978-0-12-103458-0.50015-8

Cited by 26 publications

(13 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These authors conducted an exhaustive study of the morphology and the chemical composition of these particles in Secale and Pennisetum. In other families similar structures are formed during pollen tube formation (Dickinson & Larson 1975, Cresti et al 1977, Cresti & Tiezzi 1990, Geitmann et al 1995. They are surrounded by a membrane and initially have a granular content.…”

Section: Cytoplasmmentioning

confidence: 96%

The role of the intine and cytoplasm in the activation and germination processes of Poaceae pollen grains

Marquez

Seoane-Camba

Suárez‐Cervera

1997

Grana

View full text Add to dashboard Cite

Section: Cytoplasmmentioning

confidence: 96%

The role of the intine and cytoplasm in the activation and germination processes of Poaceae pollen grains

Marquez

Seoane-Camba

Suárez‐Cervera

1997

Grana

View full text Add to dashboard Cite

“…Microscopic examination of growing pollen tubes reveals the characteristic zonation in which the apical region of the tube possesses a clear cap with more granular elements behind (26,30,54,100,115,117,137). These internal components exhibit vigorous "reverse fountain" cytoplasmic streaming (55,56,117).…”

Section: Overview Of the Pollen Tube Structurementioning

confidence: 97%

“…However, within the tip itself the motion is chaotic and turbulent, with vesicles appearing to move in a random, diffusive manner from the base of the clear zone to the extreme apex (117). More detail is provided at the electron microscope (EM) level (26,115,137), but this often necessitates chemical fixation, a process that has been shown to produce severe disorganization of cytoplasmic components in both pollen tubes (32,52) and fungal hyphae (69). For this reason, the comments in this section describe studies of pollen tubes fixed by rapid freeze fixation and freeze substitution (29,30,79,83,147) in which vital processes are halted by vitrification within a few milliseconds, preventing cytoplasmic rearrangements.…”

Section: Overview Of the Pollen Tube Structurementioning

confidence: 98%

Pollen Germination and Tube Growth

Taylor

Hepler

1997

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

726

575

View full text Add to dashboard Cite

Many aspects of Angiosperm pollen germination and tube growth are discussed including mechanisms of dehydration and rehydration, in vitro germination, pollen coat compounds, the dynamic involvement of cytoskeletal elements (actin, microtubules), calcium ion fluxes, extracellular matrix elements (stylar arabinogalactan proteins), and control mechanisms of gene expression in dehydrating and germinating pollen. We focus on the recent developments in pollen biology that help us understand how the male gamete survives and accomplishes its successful delivery to the ovule of the sperm to effect sexual reproduction.

show abstract

“…The control of callose deposition may be related to the mechanisms controlling tip-growth of pollen tubes and their polar cytoplasmic organisation (Heslop-Harrison 1987;Steer and Steer 1989;Cresti and Tiezzi 1990;Taylor and Hepler 1997). Pollen tubes of Nicotiana contain an active callose synthase (CalS; uridine-diphosphate glucose: 1,3-b-D-glucan 3-b-D-glucosyl transferase, EC 2.4.1.34) enzyme, that is distinguished from the wound-activated CalS of somatic tissues in being Ca 2+ -independent (SchluÈ pmann et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Role of a callose synthase zymogen in regulating wall deposition in pollen tubes of Nicotiana alata Link et Otto

Bacic

Read

1999

Planta

View full text Add to dashboard Cite

The callose synthase (CalS) activity of membrane preparations from cultured Nicotiana alata Link & Otto pollen tubes is increased several-fold by treatment with trypsin in the presence of digitonin, possibly due to activation of an inactive (zymogen) form of the enzyme. Active and inactive forms of CalS are also present in stylar-grown tubes. Callose deposition was ®rst detected immediately after germination of pollen grains in liquid medium, at the rim of the germination aperture. During tube growth the 3-linked glucan backbone of callose was deposited at an increasing rate, reaching a maximum of 65 mg h A1 in tubes grown from 1 g pollen. Callose synthase activity was ®rst detected immediately after germination, and then also increased substantially during tube growth. Trypsin caused activation of CalS throughout a 30-h time course of tube growth, but the degree of activation was higher for younger pollen tubes. Over a 10-fold range of callose deposition rates, the assayed CalS activity was sucient to account for the rate of callose deposition without trypsin activation, implying that the form of CalS active in isolated membranes is responsible for callose deposition in intact pollen tubes. Sucrose-density-gradient centrifugation separated a lighter, intracellular membrane fraction containing only inactive CalS from a heavier, plasma-membrane fraction containing both active and inactive CalS, with younger pollen tubes containing relatively more of the inactive intracellular enzyme. The increasing rate of callose deposition during pollen-tube growth may thus be caused by the transport of inactive forms of CalS from intracellular membranes to the plasma membrane, followed by the regulated activation of these inactive forms in this ®nal location.Abbreviations: BSA = bovine serum albumin; CalS = callose synthase; Chaps = 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulphonate; HE = Hepes-EDTA buer; PBS = phosphate-buered saline; UDP-Glc = UDP-glucose

show abstract

Germination and pollen tube formation

Cited by 26 publications

References 110 publications

The role of the intine and cytoplasm in the activation and germination processes of Poaceae pollen grains

The role of the intine and cytoplasm in the activation and germination processes of Poaceae pollen grains

Pollen Germination and Tube Growth

Role of a callose synthase zymogen in regulating wall deposition in pollen tubes of Nicotiana alata Link et Otto

Contact Info

Product

Resources

About