2018
DOI: 10.1515/contagri-2018-0028
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Germination and Protocorm Formation of Ophrys Sphegodes Mill. – In Vitro Protocol for a Rare Orchid Species

Abstract: Summary Ophrys sphegodes Mill. is a wild orchid species which is threatened and protected due to its pollination biology, small seed and habitat destruction. The aim of this study was to establish asymbiotic germination protocol for the purpose of ex situ conservation. Two basal media Knudson C (KC) and Malmgren (MM), supplemented with organic additives (peptone (PE), L-glutamin (A)e, folic acid, casein hydrolysate (CA)) added separately and control media KC--C and MM--C were used in the present research. All … Show more

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Cited by 5 publications
(3 citation statements)
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“…To test asymbiotic seed germination of four Anacamptis species, Magrini et al [44] com-pared several culture media differing mainly in the nitrogen source, organic or inorganic: the medium BM-1 with only amino acids as nitrogen source promotes germination and supports seedlings growth. Similar results have been obtained by Dulić et al [45] on Ophrys sphegodes, confirming that asymbiotic germination and protocorm formation is promoted by organic nitrogen. Moreover, our results confirmed the negative effects of inorganic sources of nitrogen (nitrate and ammonium salts), in accordance with previous studies on other species of temperate terrestrial orchids [46][47][48].…”
Section: Discussionsupporting
confidence: 90%
“…To test asymbiotic seed germination of four Anacamptis species, Magrini et al [44] com-pared several culture media differing mainly in the nitrogen source, organic or inorganic: the medium BM-1 with only amino acids as nitrogen source promotes germination and supports seedlings growth. Similar results have been obtained by Dulić et al [45] on Ophrys sphegodes, confirming that asymbiotic germination and protocorm formation is promoted by organic nitrogen. Moreover, our results confirmed the negative effects of inorganic sources of nitrogen (nitrate and ammonium salts), in accordance with previous studies on other species of temperate terrestrial orchids [46][47][48].…”
Section: Discussionsupporting
confidence: 90%
“…It is not endemic but has vulnerable (VU) category (Bongers, 1993;Sezik et al, 2007). Both species (H. robertianum and O. italica) are strictly protected by "The Convention on International Trade in Endangered Species of Wild Fauna and Flora" (CITES) convention (Dulıć et al, 2018).…”
Section: H Robertianummentioning
confidence: 99%
“…The plants grow 60-80 cm tall (Davis, 1984;Delfolge, 2006;Rossi, 2002;Teoh, 2016), are easy to find, and are valuable for salep production. Unfortunately, due to the overharvesting of their tubers for salep production, many species in this genus are locally threatened in Türkiye (Dulić et al, 2018;Teoh, 2019).…”
Section: Introductionmentioning
confidence: 99%