Germination and reproductive biology in salty conditions of Asplenium marinum (Aspleniaceae), a European coastal fern

Pangua, Emilia; Belmonte, Román; Pajarón, Santiago

doi:10.1016/j.flora.2008.09.007

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…Salinity is one of the most studied stress factors, affecting also prothallus development. Higher salinity of the substrate produces a significant decrease in gametophyte sizes, and affect the development of gametangia as well (Pangua et al, 2009). D. affinis gametophyte contains proteins to overcome salt stress including SALT OVERLY SENSITIVE 1 ( SOS1 ), one of the best known.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analyses in the gametophyte of Dryopteris affinis: apomixis and more

Ojosnegros,

Alvarez,

Gagliardini

et al. 2024

Preprint

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The gametophyte of the fern Dryopteris affinis ssp. affinis represents a good model to explore the molecular basis of vegetative and reproductive development, as well as stress responses. Specifically, this fern reproduces asexually by apogamy, a peculiar case of apomixis whereby a sporophyte forms directly from a gametophytic cell without fertilization. Using an RNA-sequencing approach, we have previously annotated more than six thousand transcripts. Here, we selected one hundred of the inferred proteins that seemed particularly interesting for a detailed study of their potential functions, protein-protein interactions, and molecular phylogenies. As expected, a plethora of proteins associated with gametogenesis and embryogenesis in angiosperms, such as FERONIA (FER) and CHROMATING REMODELING 11 (CHR11) were identified, and more than a dozen candidates potentially involved in apomixis, such as ARGONAUTE4 (AGO4), AGO9, and AGO10, BABY BOOM (BBM), FASCIATED STEM4 (FAS4), FERTILIZATION-INDEPENDENT ENDOSPERM (FIE), and MATERNAL EFFECT EMBRYO ARREST29 (MEE29). In addition, proteins involved in the response to biotic and abiotic stresses were widely represented, as shown by the enrichment of heat-shock proteins. Using the String platform, studying interactomes revealed that most of the protein-protein interactions were predicted based on experimental, database, and text mining datasets, with MULTICOPY SUPPRESSOR OF IRA4 (MSI4) showing the highest number of 16 interactions. Lastly, some proteins were studied from a phylogenetic point of view, comparing the alignments with respect to more distantly or closely related plant groups, identifying AGO1 as the evolutionarily most similar to that other ferns and the most distant to the predicted common ancestor. This work sets the stage for future functional characterizations in relation to gametophyte development including apomictic reproduction.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analyses in the gametophyte of Dryopteris affinis: apomixis and more

Ojosnegros,

Alvarez,

Gagliardini

et al. 2024

Preprint

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“…Asplenium marinum and Acrostichum sp. [ 25 - 27 ]). Long-term storage experiments should be performed also for these species to test whether they are indeed better capable of surviving saline inundated conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Bogdanović et al [ 24 ] showed reduced chlorophyll content in gametophytes of various ferns and mosses when grown on very saline media (>100 mM NaCl). Reduced germination, gametophytic growth and gametangial development on saline substrates have been found even for the halophytic species Asplenium marinum [ 25 ], Acrostichum aureum and Acrostichum danaeifolium [ 26 , 27 ]. We did not find any study that investigated the effect of saline incubation on spore viability.…”

Section: Introductionmentioning

confidence: 99%

Fern Spore Longevity in Saline Water: Can Sea Bottom Sediments Maintain a Viable Spore Bank?

Groot

During

2013

PLoS ONE

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Freshwater and marine sediments often harbor reservoirs of plant diaspores, from which germination and establishment may occur whenever the sediment falls dry. Therewith, they form valuable records of historical inter- and intraspecific diversity, and are increasingly exploited to facilitate diversity establishment in new or restored nature areas. Yet, while ferns may constitute a considerable part of a vegetation’s diversity and sediments are known to contain fern spores, little is known about their longevity, which may suffer from inundation and - in sea bottoms - salt stress. We tested the potential of ferns to establish from a sea or lake bottom, using experimental studies on spore survival and gametophyte formation, as well as a spore bank analysis on sediments from a former Dutch inland sea. Our experimental results revealed clear differences among species. For Asplenium scolopendrium and Gymnocarpium dryopteris, spore germination was not affected by inundated storage alone, but decreased with rising salt concentrations. In contrast, for Asplenium trichomanes subsp. quadrivalens germination decreased following inundation, but not in response to salt. Germination rates decreased with time of storage in saline water. Smaller and less viable gametophytes were produced when saline storage lasted for a year. Effects on germination and gametophyte development clearly differed among genotypes of A. scolopendrium. Spore bank analyses detected no viable spores in marine sediment layers. Only two very small gametophytes (identified as Thelypteris palustris via DNA barcoding) emerged from freshwater sediments. Both died before maturation. We conclude that marine, and likely even freshwater sediments, will generally be of little value for long-term storage of fern diversity. The development of any fern vegetation on a former sea floor will depend heavily on the deposition of spores onto the drained land by natural or artificial means of dispersal.

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“…Capsules F. hygrometrica after sterilization were immersed in 3.26% NaCl solution equal to 100% sea water, according to the standard described by Pangua et al (2009). Five capsules were selected for each treatment consisting in control without influence of sea water and treatment with capsules immersed in sea water for 6, 12, 18 and 24min.…”

Section: Experimental Designmentioning

confidence: 99%

Growth and development of halophyte Funaria hygrometrica Hedw. (Funariaceae) under salt stress

Vargas¹,

Minozzo²,

Pereira³

et al. 2017

Biosci. J.

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ABSTRACT:The ability of bryophytes to tolerate salt is determined by a number of biochemical routes, whereas the salt ends up driving the activation of adaptive responses to tolerate this adverse condition. Salinity is the main limiting environmental factor under plant development, and is caused by excess salt ions in the environment, mainly Na + and Cl -. The optimal growth of plants in saline environment is obtained in concentrations of 50% of NaCl. Due to these findings, the importance of the study of the effect of salinity on the germination of plants, in this case in Funaria hygrometrica Hedw., is noticed.

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Germination and reproductive biology in salty conditions of Asplenium marinum (Aspleniaceae), a European coastal fern

Cited by 7 publications

References 29 publications

Transcriptomic analyses in the gametophyte of Dryopteris affinis: apomixis and more

Transcriptomic analyses in the gametophyte of Dryopteris affinis: apomixis and more

Fern Spore Longevity in Saline Water: Can Sea Bottom Sediments Maintain a Viable Spore Bank?

Growth and development of halophyte Funaria hygrometrica Hedw. (Funariaceae) under salt stress

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