Germplasm with Resistance to Potato virus Y Derived from Solanum chacoense: Clones M19 (39–7) and M20 (XD3)

Fulladolsa, Ana Cristina; Charkowski, Amy O.; Cai, Xingkui; Whitworth, Jonathan L.; Gray, Stewart M.; Jansky, Shelley

doi:10.1007/s12230-019-09719-6

Cited by 7 publications

(2 citation statements)

References 24 publications

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“…Cycle 1 was initiated with the goal of identifying a fertile F 2 clone homozygous for Sli to be used as the male parent in a modified backcrossing scheme with S. tuberosum dihaploids as the recurrent genetic background (Figure 1). The grandparents of the F 2 population were an “heirloom” S. tuberosum dihaploid US‐W4 (Peloquin & Hougas, 1960) and an inbred clone M19 from the wild species S. chacoense (Fulladolsa et al., 2019). At that time (2018), it was believed that introgression of Sli from S. chacoense into S. tuberosum was necessary, and thus our strategy was to identify self‐fertile F 2 clones homozygous for the M19 haplotype in the vicinity of the published location of Sli on chromosome 12.…”

Section: Resultsmentioning

confidence: 99%

Using haplotype and QTL analysis to fix favorable alleles in diploid potato breeding

Song

Endelman

2023

The Plant Genome

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At present, the potato (Solanum tuberosum L.) of international commerce is autotetraploid, and the complexity of this genetic system creates limitations for breeding. Diploid potato breeding has long been used for population improvement, and because of an improved understanding of the genetics of gametophytic self‐incompatibility, there is now sustained interest in the development of uniform F1 hybrid varieties based on inbred parents. We report here on the use of haplotype and quantitative trait locus (QTL) analysis in a modified backcrossing (BC) scheme, using primary dihaploids of S. tuberosum as the recurrent parental background. In Cycle 1, we selected XD3‐36, a self‐fertile F2 individual homozygous for the self‐compatibility gene Sli (S‐locus inhibitor). Signatures of gametic and zygotic selection were observed at multiple loci in the F2 generation, including Sli. In the BC1 cycle, an F1 population derived from XD3‐36 showed a bimodal response for vine maturity, which led to the identification of late versus early alleles in XD3‐36 for the gene CDF1 (Cycling DOF Factor 1). Greenhouse phenotypes and haplotype analysis were used to select a vigorous and self‐fertile F2 individual with 43% homozygosity, including for Sli and the early‐maturing allele CDF1.3. Partially inbred lines from the BC1 and BC2 cycles have been used to initiate new cycles of selection, with the goal of reaching higher homozygosity while maintaining plant vigor, fertility, and yield.

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Section: Resultsmentioning

confidence: 99%

Using haplotype and QTL analysis to fix favorable alleles in diploid potato breeding

Song

Endelman

2023

The Plant Genome

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show abstract

“…1). The grandparents of the F 2 population were an “heirloom” S. tuberosum dihaploid US-W4 (Peloquin & Hougas, 1960) and an inbred clone M19 from the wild species S. chacoense (Fulladolsa et al, 2019). At that time (2018), it was believed that introgression of Sli from S. chacoense into S. tuberosum was necessary, and thus our strategy was to identify self-fertile F 2 clones homozygous for the M19 haplotype in the vicinity of the published location of Sli on chromosome 12.…”

Section: Resultsmentioning

confidence: 99%

Using Haplotype and QTL Analysis to Fix Favorable Alleles in Diploid Potato Breeding

Song

Endelman

2022

Preprint

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At present, the potato of international commerce is autotetraploid, and the complexity of this genetic system creates limitations for breeding. Diploid potato breeding has long been used for population improvement, and thanks to improved understanding of the genetics of gametophytic self-incompatibility, there is now sustained interest in the development of uniform F1 hybrid varieties based on inbred parents. We report here on the use of haplotype and QTL analysis in a modified backcrossing (BC) scheme, using primary dihaploids ofS.tuberosumas the recurrent parental background. In Cycle 1 we selected XD3-36, a self-fertile F2 clone homozygous for the self-compatibility geneSli. Signatures of gametic and zygotic selection were observed at multiple loci in the F2 generation, includingSli. In the BC1 cycle, an F1 population derived from XD3-36 showed a bimodal response for vine maturity, which led to the identification of late vs. early alleles in XD3-36 for the geneStCDF1 (Cycling DOF Factor 1). Greenhouse phenotypes and haplotype analysis were used to select a vigorous and self-fertile F2 individual with 43% homozygosity, including forSliand the early-maturing alleleStCDF1.3. Partially inbred lines from the BC1 and BC2 cycles have been used to initiate new cycles of selection, with the goal of reaching higher homozygosity while maintaining plant vigor, fertility, and yield.

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Improving Resistance to Diseases and Pests: A Dynamic Situation

Bradshaw¹

2021

Potato Breeding: Theory and Practice

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Germplasm with Resistance to Potato virus Y Derived from Solanum chacoense: Clones M19 (39–7) and M20 (XD3)

Cited by 7 publications

References 24 publications

Using haplotype and QTL analysis to fix favorable alleles in diploid potato breeding

Using haplotype and QTL analysis to fix favorable alleles in diploid potato breeding

Using Haplotype and QTL Analysis to Fix Favorable Alleles in Diploid Potato Breeding

Improving Resistance to Diseases and Pests: A Dynamic Situation

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