Improving Resistance to Diseases and Pests: A Dynamic Situation

Bradshaw, J. E.

doi:10.1007/978-3-030-64414-7_5

Cited by 2 publications

(3 citation statements)

References 262 publications

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“…Although F v /F m is the most common fluorescence parameter used in plant stress studies [27,28], the results of this study show that F v /F m ranged from 0.79 to 0.81 for both PVM and PVM + PVS plants, indicating good fitness of plants. As noted by [15,19], plants infected with PVM and PVS do not always show symptoms, which depends on the variety and virus isolates. Considering the multispectral parameters, mixed infection significantly decreases the chlorophyll index (CHI) and increases the reflectance in the far-red (R FarRed ) (Table 6).…”

Section: The Influence Of Sanitary Status Of Plants On Chlorophyll Fl...mentioning

confidence: 95%

“…PVM and PVS can be transmitted by aphids, especially the green peach aphid (Myzus persicae), by vegetative propagation (via tubers), or mechanically (e.g., through contaminated tools and wounds) [17,18]. Plants infected with PVM and PVS do not always show symptoms, depending on the cultivar and virus isolates [9,15,19]. However, PVM can reduce tuber yield by 11-45% [11,20], and PVS by 10-20% [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Partial Elimination of Viruses from Traditional Potato Cultivar ‘Brinjak’ by Chemotherapy and Its Impact on Physiology and Yield Components

Kereša

Vončina²,

Lazarević³

et al. 2022

Horticulturae

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Viruses are responsible for more than 50% of annual potato tuber yield losses and cause great economic damage. The traditional Croatian potato cultivar ‘Brinjak’ is important for local growers because of its economically profitable production and as a gene pool for future breeding programs. However, the full genetic potential of the cultivar cannot be exploited due to virus infection. In this study, we attempted to eliminate potato virus M (PVM) and potato virus S (PVS) from potato cultivar ‘Brinjak’ and to evaluate the effects on physiological parameters and yield. Shoot apices were isolated from PVM + PVS-infected sprouts and cultivated for six weeks on MS medium with the addition of 50 or 100 mg L−1 ribavirin. The surviving shoot apices were micropropagated. The in vitro post-eradication period lasted 200 days. DAS-ELISA and RT-PCR were performed on R0 and R1 plants 90 days after acclimatization to determine the sanitary status of the plants. Chlorophyll fluorescence and multispectral imaging were performed on the R0 plants at the same time. The success of PVS elimination was 33% at both ribavirin concentrations. However, neither concentration was successful in eliminating PVM. Plants with mixed infection (PVM + PVS) had more severe disease symptoms compared to PVM-infected plants, affecting photochemistry and multispectral parameters and, consequently, yield. PVM + PVS plants had significantly lower number and weight of tubers per plant and lower average tuber weight than plants with single PVM infection in most of the generations studied. The results indicate a strong negative impact of PVS in mixed infections with PVM and show the importance of its elimination from potato plants.

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Section: The Influence Of Sanitary Status Of Plants On Chlorophyll Fl...mentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Partial Elimination of Viruses from Traditional Potato Cultivar ‘Brinjak’ by Chemotherapy and Its Impact on Physiology and Yield Components

Kereša

Vončina²,

Lazarević³

et al. 2022

Horticulturae

View full text Add to dashboard Cite

show abstract

“…PVS and PVA are also transmitted by contact ( Valkonen, 2007 ; Fuentes et al, 2021 ). Plants infected by PVM and PVS may not always show symptoms depending on the cultivar and virus isolates ( Kreuze et al, 2020 ; Bradshaw, 2021 ). Furthermore, mixed infection can increase symptom severity and virus accumulation ( Nyalugwe et al, 2012 ; Moreno and López-Moya, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

et al. 2022

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Certain viruses dramatically affect yield and quality of potatoes and have proved difficult to eradicate with current approaches. Here, we describe a reliable and efficient virus eradication method that is high throughput and more efficacious at producing virus-free potato plants than current reported methods. Thermotherapy, chemotherapy, and cryotherapy treatments were tested alone and in combination for ability to eradicate single and mixed Potato virus S (PVS), Potato virus A (PVA), and Potato virus M (PVM) infections from three potato cultivars. Chemotherapy treatments were undertaken on in vitro shoot segments for four weeks in culture medium supplemented with 100 mg L−1 ribavirin. Thermotherapy on in vitro shoot segments was applied for two weeks at 40°C (day) and 28°C (night) with a 16 h photoperiod. Plant vitrification solution 2 (PVS2) and cryotherapy treatments included a shoot tip preculture followed by exposure to PVS2 either without or with liquid nitrogen (LN, cryotherapy) treatment. The virus status of control and recovered plants following therapies was assessed in post-regeneration culture after 3 months and then retested in plants after they had been growing in a greenhouse for a further 3 months. Microtuber production was investigated using in vitro virus-free and virus-infected segments. We found that thermotherapy and cryotherapy (60 min PVS2 + LN) used alone were not effective in virus eradication, while chemotherapy was better but with variable efficacy (20–100%). The most effective result (70–100% virus eradication) was obtained by combining chemotherapy with cryotherapy, or by consecutive chemotherapy, combined chemotherapy and thermotherapy, then cryotherapy treatments irrespective of cultivar. Regrowth following the two best virus eradication treatments was similar ranging from 8.6 to 29% across the three cultivars. The importance of virus removal on yield was reflected in “Dunluce” free of PVS having higher numbers of microtubers and in “V500’ free of PVS and PVA having a greater proportion of microtubers > 5 mm. Our improved procedure has potential for producing virus-free planting material for the potato industry. It could also underpin the global exchange of virus-free germplasm for conservation and breeding programs.

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Improving Resistance to Diseases and Pests: A Dynamic Situation

Cited by 2 publications

References 262 publications

Partial Elimination of Viruses from Traditional Potato Cultivar ‘Brinjak’ by Chemotherapy and Its Impact on Physiology and Yield Components

Partial Elimination of Viruses from Traditional Potato Cultivar ‘Brinjak’ by Chemotherapy and Its Impact on Physiology and Yield Components

Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-Grown Potato Shoots Using in vitro Therapies

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