gga-miR-200b-3p Promotes Macrophage Activation and Differentiation via Targeting Monocyte to Macrophage Differentiation-Associated in HD11 Cells

Lin, Wencheng; Zhou, Lianghui; Liu, Yijie; Zhang, Danmeng; Yan, Yongde; Chang, Yung-Fu; Zhang, Xiquan; Xie, Qingmei; Luo, Qianyi

doi:10.3389/fimmu.2020.563143

Cited by 8 publications

(8 citation statements)

References 54 publications

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“…Previously, we confirmed that MMD , also a DEmRNA in this study, was associated with macrophage activation and differentiation ( 40 ). We inferred that certain DEcircRNAs were functional as miRNA sponges to promote MMD expression.…”

Section: Resultssupporting

confidence: 89%

“…MMD was associated with macrophage activation and differentiation ( 40 ). To explore the role of circMGAT5-miR-132c-5p-MMD axis in macrophage activation and differentiation.…”

Section: Resultsmentioning

confidence: 99%

“…It was reported that MMD was preferentially expressed in mature macrophages and may affect the activation and differentiation of macrophages ( 41 ). Indeed, our previous work revealed that the knockdown of MMD upregulated expression of macrophage surface and activation markers, such as F4/80 and MHC-II ( 42 – 44 ), indicating that MMD could inhibit macrophage activation and differentiation ( 40 ). Here, circMGAT5 was able to elevate MMD expression suggesting that circMGAT5 might play a potential role in macrophage activation and differentiation.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, our results confirmed the target relationship between miR-132c-5p and MMD. CircMGAT5 regulates macrophage activation and differentiation through the circMGAT5-miR-132c-5p-MMD axis MMD was associated with macrophage activation and differentiation (40). To explore the role of circMGAT5-miR-132c-5p-MMD axis in macrophage activation and differentiation.…”

Section: Identification Of Circmgat5-mir-132c-5p-mmd Regulatory Networkmentioning

confidence: 99%

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Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis

Chen

Wang²,

Chen³

et al. 2022

Front. Immunol.

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Avian coccidiosis is a common enzootic disease caused by infection of Eimeria species parasites. It causes huge economic losses in the global poultry industry. Current control using anticoccidial drugs or vaccination is limited due to drug resistance and the relatively high cost of vaccines. Improving host genetic resistance to Eimeria species is considered an effective strategy for improved control of coccidiosis. Circular RNAs (circRNAs) have been found to function as biomarkers or diagnoses of various kinds of diseases. The molecular biological functions of circRNAs, miRNAs, and mRNAs related to Sasso chicken have not yet been described during Eimeria species challenge. In this study, RNA-seq was used to profile the expression pattern of circRNAs, miRNAs, and mRNAs in spleens from Eimeria tenella-infected and non-infected commercial dual-purpose Sasso T445 breed chickens. Results showed a total of 40 differentially expressed circRNAs (DEcircRNAs), 31 differentially expressed miRNAs (DEmiRNAs), and 820 differentially expressed genes (DEmRNAs) between infected and non-infected chickens. Regulatory networks were constructed between differentially expressed circRNAs, miRNAs, and mRNAs to offer insights into the interaction mechanisms between chickens and Eimeria spp. Functional validation of a significantly differentially expressed circRNA, circMGAT5, revealed that circMGAT5 could sponge miR-132c-5p to promote the expression of the miR-132c-5p target gene monocyte to macrophage differentiation-associated (MMD) during the infection of E. tenella sporozoites or LPS stimulation. Pathologically, knockdown of circMGAT5 significantly upregulated the expression of macrophage surface markers and the macrophage activation marker, F4/80 and MHC-II, which indicated that circMGAT5 might inhibit the activation of macrophage. miR-132c-5p markedly facilitated the expression of F4/80 and MHC-II while circMGAT5 could attenuate the increase of F4/80 and MHC-II induced by miR-132c-5p, indicating that circMGAT5 exhibited function through the circMGAT5-miR-132c-5p-MMD axis. Together, our results indicate that circRNAs exhibit their resistance or susceptive roles during E. tenella infection. Among these, circMGAT5 may inhibit the activation of macrophages through the circMGAT5-miR-132c-5p-MMD axis to participate in the immune response induced by Eimeria infection.

show abstract

Section: Resultssupporting

confidence: 89%

“…MMD was associated with macrophage activation and differentiation ( 40 ). To explore the role of circMGAT5-miR-132c-5p-MMD axis in macrophage activation and differentiation.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Identification Of Circmgat5-mir-132c-5p-mmd Regulatory Networkmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis

Chen

Wang²,

Chen³

et al. 2022

Front. Immunol.

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show abstract

“…MMD, first discovered in 1995, is named for its high expression in mature macrophages but not in monocytes ( Rehli et al, 1995 ). Preferentially expressed in mature macrophages, MMD participates in the activation of macrophages with a carcinogenic role ( Liu et al, 2012 ; Lin et al, 2020 ). There was a research that highlighted the value of miR-140–5p/MMD axis in non-small cell lung cancer ( Li and He 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Screening of ferroptosis-related genes with prognostic effect in colorectal cancer by bioinformatic analysis

Zhou

Wang

et al. 2022

Front. Mol. Biosci.

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Colorectal cancer (CRC) remains a common malignant tumor of digestive tract with high incidence rate and high mortality in the worldwide. The current clinical treatments of CRC often fail to achieve satisfactory results. Searching for more effective prediction or prognosis biomarkers, or developing more targeted therapeutic schedule may help to improve the outcomes of CRC patients. Here, we tried to study the effect of ferroptosis-related genes on CRC prognosis and make it clearer that ferroptosis has connection with immune environment. First, we obtained gene expression data of CRC and normal tissues, as well as corresponding clinical data from the Gene Expression Omnibus (GEO) database and the Cancer Genome Atlas (TCGA) database. The differentially expressed genes (DEGs) were intersected with ferroptosis-related gene set downloaded from FerrDb database, and 93 abnormally expressed ferroptosis-related genes were obtained. Then, these genes were analyzed for functional enrichment. Univariate Cox regression and multivariate Cox regression analyses were performed to establish prognostic model based on ferroptosis-related genes. In the process of exploring the correlation between prognostic genes and immune infiltration, we found that these genes were closely related to B cells, CD8+ T cells, CD4+ T cells, macrophages and other cells in CRC. In addition, we found a large proportion of plasma cells and macrophages in TCGA-COADREAD. Finally, a prognostic nomogram of ferroptosis-related genes was established, including age, sex, grade and other predicted values. To summary, we established a prognostic model of colorectal cancer (CRC) based on ferroptosis-related genes and further explored the relationship between these genes with immune microenvironment.

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Transcriptome analysis reveals that hydrogen sulfide exposure suppresses cell proliferation and induces apoptosis through ciR-PTPN23/miR-15a/E2F3 signaling in broiler thymus

Chi²,

Liu³

et al. 2021

Environmental Pollution

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gga-miR-200b-3p Promotes Macrophage Activation and Differentiation via Targeting Monocyte to Macrophage Differentiation-Associated in HD11 Cells

Cited by 8 publications

References 54 publications

Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis

Transcriptome analysis of differentially expressed circRNAs miRNAs and mRNAs during the challenge of coccidiosis

Screening of ferroptosis-related genes with prognostic effect in colorectal cancer by bioinformatic analysis

Transcriptome analysis reveals that hydrogen sulfide exposure suppresses cell proliferation and induces apoptosis through ciR-PTPN23/miR-15a/E2F3 signaling in broiler thymus

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