GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae

Xiao, Shenghua; Hu, Qin; Shen, Jili; Liu, Shiming; Yang, Ziyi; Chen, Kun; Klosterman, Steven J.; Javornik, Branka; Zhang, Xianlong; Zhu, Longfu

doi:10.1007/s00299-021-02672-x

Cited by 43 publications

(23 citation statements)

References 93 publications

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“…The FaBG3, which encodes β-glucosidase in F. ananassa , has been shown to regulate fruit ripening, dehydration stress, and B. cinerea fungal infection in strawberries via modulation of ABA homeostasis and transcriptional regulation of ripening-related genes (Li et al, 2013 ). The previous reports have indicated that lignin is deposited at the infection site to inhibit the penetration and growth of pathogens (Mutuku et al, 2019 ; Lee et al, 2020 ; Xiao et al, 2021 ), and relevant genes, such as PALs, CAD , and COMTs, have been confirmed, through functional genomics experiments, to be associated with disease-resistance (Tonnessen et al, 2015 ; Li et al, 2019 ; Hoch et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Molecular Signatures Associated With Resistance to Botrytis cinerea in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis

et al. 2022

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Gray mold caused by Botrytis cinerea, which is considered to be the second most destructive necrotrophic fungus, leads to major economic losses in strawberry (Fragaria × ananassa) production. B. cinerea preferentially infects strawberry flowers and fruits, leading to flower blight and fruit rot. Compared with those of the fruit, the mechanisms of flower defense against B. cinerea remain largely unexplored. Therefore, in this study, we aimed to unveil the resistance mechanisms of strawberry flower through dynamic and comparative transcriptome analysis with resistant and susceptible strawberry cultivars. Our experimental data suggest that resistance to B. cinerea in the strawberry flower is probably regulated at the transcriptome level during the early stages of infection and strawberry flower has highly complex and dynamic regulatory networks controlling a multi-layered defense response to B. cinerea. First of all, the higher expression of disease-resistance genes but lower expression of cell wall degrading enzymes and peroxidases leads to higher resistance to B. cinerea in the resistant cultivar. Interestingly, CPKs, RBOHDs, CNGCs, and CMLs comprised a calcium signaling pathway especially play a crucial role in enhancing resistance by increasing their expression. Besides, six types of phytohormones forming a complex regulatory network mediated flower resistance, especially JA and auxin. Finally, the genes involved in the phenylpropanoid and amino acids biosynthesis pathways were gene sets specially expressed or different expression genes, both of them contribute to the flower resistance to B. cinerea. These data provide the foundation for a better understanding of strawberry gray mold, along with detailed genetic information and resistant materials to enable genetic improvement of strawberry plant resistance to gray mold.

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Section: Discussionmentioning

confidence: 99%

Deciphering the Molecular Signatures Associated With Resistance to Botrytis cinerea in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis

et al. 2022

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“…In addition, the induced lignification by pathogen invasion further inhibits the growth, movement and infection ability of the pathogens ( Xie et al, 2018 ; Li et al, 2020 ). In cotton, lignin biosynthesis is significantly induced upon V. dahliae infection and has a significant role in resistance to V. dahliae ( Zhu et al, 2018 ; Li et al, 2020 ; Xiao et al, 2021 ; Xiong et al, 2021 ). Therefore, it is important to identify the genes responsible for the V. dahliae induced lignification.…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptomic study in cotton has also found V. dahliae induced up-regulation of lignin biosynthesis genes ( Xu et al, 2011 ). Most recently, GhMYB4 and Gh4CL30 were reported to regulate Verticillium wilt resistance in cotton by interfering lignification ( Xiao et al, 2021 ; Xiong et al, 2021 ). Salicylic acid (SA) and jasmonic acid (JA) are necessary for the activation and mediation of plant defense signaling and responses to various biotic stresses ( Loake and Grant, 2007 ; Miao et al, 2019 ; Zheng et al, 2019 ; Song et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Cinnamyl Alcohol Dehydrogenase Encoding Genes Involved in Lignin Biosynthesis and Resistance to Verticillium dahliae in Upland Cotton (Gossypium hirsutum L.)

Zhang

Zhao

et al. 2022

Front. Plant Sci.

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Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is one of the most devastating diseases in cotton (Gossypium spp.). Lignin in the cell wall forms a physical barrier to inhibit pathogen invasion, and defense-induced lignification reinforces secondary cell wall to prevent pathogens from further spreading. Cinnamyl alcohol dehydrogenases (CADs) catalyze the production of three main monolignols, p-coumaryl- (H), coniferyl- (G), and sinapyl-alcohols (S), which are the fundamental blocks of lignin. Here, we identified CAD genes in G. hirsutum, analyzed their expression profiles in cotton leaf, stem, and root from different developmental stages, and selected GhCAD35, GhCAD45, and GhCAD43, which were consistently induced by V. dahliae inoculation in G. hirsutum cultivars resistant or susceptible to V. dahliae. On the basis of confirmation of the in vitro enzymatic activity of the three proteins in generation of the three monolignols, we used virus-induced gene silencing (VIGS) to investigate the effects of silencing of GhCAD35, GhCAD45, or GhCAD43 on resistance to V. dahliae as well as on deposition and the composition of lignin. Silencing each of the three CADs impaired the defense-induced lignification and salicylic acid biosynthesis in stem, and compromised resistance to V. dahliae. Moreover, our study showed that silencing the three GhCADs severely affected the biosynthesis of S-lignin, leading to a decrease of the syringyl/guaiacyl (S/G) ratio. Heterogeneous overexpression of GhCAD35, GhCAD45, or GhCAD43 in Arabidopsis enhanced disease resistance. Taken together, our study demonstrates a role of the three GhCADs in defense-induced lignin biosynthesis and resistance to V. dahliae in G. hirsutum.

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“…GhMYB4, transcription factors induce resistance against verticillium dahlia in cotton. It provide a great potential for the improvement in breeding of cotton plants [56]. High temperature induces the MYB transcriptional factor and positive regulator of thermotolerance [54].…”

Section: Myb Transcription Factors and Plant Defense Mechanismsmentioning

confidence: 99%

Recent Advanced Metabolic and Genetic Engineering of Phenylpropanoid Biosynthetic Pathways

Anwar

Chen

Xiao

et al. 2021

IJMS

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The MYB transcription factors (TFs) are evolving as critical role in the regulation of the phenylpropanoid and tanshinones biosynthetic pathway. MYB TFs relate to a very important gene family, which are involved in the regulation of primary and secondary metabolisms, terpenoids, bioactive compounds, plant defense against various stresses and cell morphology. R2R3 MYB TFs contained a conserved N-terminal domain, but the domain at C-terminal sorts them different regarding their structures and functions. MYB TFs suppressors generally possess particular repressive motifs, such as pdLNLD/ELxiG/S and TLLLFR, which contribute to their suppression role through a diversity of complex regulatory mechanisms. A novel flower specific “NF/YWSV/MEDF/LW” conserved motif has a great potential to understand the mechanisms of flower development. In the current review, we summarize recent advanced progress of MYB TFs on transcription regulation, posttranscriptional, microRNA, conserved motif and propose directions to future prospective research. We further suggest there should be more focus on the investigation for the role of MYB TFs in microalgae, which has great potential for heterologous protein expression system for future perspectives.

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GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae

Cited by 43 publications

References 93 publications

Deciphering the Molecular Signatures Associated With Resistance to Botrytis cinerea in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis

Deciphering the Molecular Signatures Associated With Resistance to Botrytis cinerea in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis

Identification and Characterization of Cinnamyl Alcohol Dehydrogenase Encoding Genes Involved in Lignin Biosynthesis and Resistance to Verticillium dahliae in Upland Cotton (Gossypium hirsutum L.)

Recent Advanced Metabolic and Genetic Engineering of Phenylpropanoid Biosynthetic Pathways

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