Ghrelin Detection Using Spiegelmer‐Capture Molecules

Mascini, Marcello; Papamichael, Katerina I.; Mevola, Isabella; Pravda, Miloslav; Guilbault, George G.

doi:10.1080/00032710601102391

Cited by 19 publications

(11 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A label-free and reagentless signal-off aptasensor used spiegelmers, a new generation of aptamers with increased stability [4]. Spiegelmers are enantiomeric aptamers composed of l-nucleotides, resistant to nucleases, showing an extraordinary stability in biological fluids.…”

Section: Aptamer Label-free Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Aptasensors

2009

View full text Add to dashboard Cite

This review presents electrochemical aptasensors reported in the literature. Their development has been growing as evidenced by the increasing number of scientific publications on this subject since 2004. Aptasensors are presented according to their transduction mode, including the most described amperometric and impedimetric devices as well as FETs and the first very recently reported potentiometric aptasensor. Signal-on (positive readout signal) and less sensitive signal-off (negative readout signal) aptasensors are reviewed based on target binding-induced conformational change of aptamers or on target binding-induced strand displacement or even on both processes. Aptamerlabeled and aptamer label-free devices are presented separately.

show abstract

Section: Aptamer Label-free Detectionmentioning

confidence: 99%

“…The major limitation is due to RNA aptamer degradation by ribonucleases. This can be overcome by chemical modification of RNA aptamers or use of enantiomeric aptamers composed of l-nucleotides, known as spiegelmers [4].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensors

2009

View full text Add to dashboard Cite

show abstract

“…An improvement was obtained lowering the label concentration. Further optimizations showed that the coating concentration of ghrelin could not be reduced below 3 mg/ml without subsequent loss of signal to levels below 0.3 AU (Mascini et al 2007). All the conditions optimized so far were then used to develop an improved competition assay.…”

Section: Elona Detection Systemmentioning

confidence: 97%

Colorimetric Microarray Detection System for Ghrelin Using Aptamer‐Technology

Mascini

Guilbault

Lebrun³

et al. 2007

Analytical Letters

Self Cite

View full text Add to dashboard Cite

The aim of this research was to compare the efficiency in term of miniaturization, multiplexing, reproducibility and sensitivity of colorimetric microarray versus an enzyme linked oligonucleotide assay (ELONA).A competition assay was performed to explore the complex formation between 5 0 -biotin NOX-B11, a special aptamer called spiegelmer, and ghrelin, a growth hormone releasing acylated peptide from stomach.The aptamer technology is a very powerful method suitable for large-scale synthesis and overcoming the disadvantages of antibodies (unstable, expensive to produce, and so on) while remaining as efficient as antibodies in binding to the target. The aptamer used in this work was based on spiegelmer oligonucleotides approach that has been considered as one of the simplest, most straightforward, and cost-effective method to develop artificial receptors.The efforts were concentrated on the development of the microarray process which includes the study of the label binding condition and all analytical parameters like concentration, time and temperature involved in the interaction between the target and the ligand. The analytical signal was acquired using a flatbed scanner and elaborated with an image software that coupled with the microarray equipments led to an extremely low cost analytical device.A sigmoidal dose-response curve was obtained using the microarray system with an EC 50 of 6.1 ng/ml in a huge linear range from 0.2 up to 245.5 ng/ml with a detection limit of 0.2 ng/ml and an averaged relative standard deviation (RSD) across all the points of the curve of 6.9%. In the ELONA assay we found an EC 50 of 40.0 ng/ml, a detection limit of 2.6 ng/ml with a RSD of 9.9% in a linear range relatively narrow extending between 15.0 and 130.5 ng/ml, with the lower limit almost 45 times higher than that obtained using microarray system. The undoubtedly better performance of the microarray system developed was demonstrated. The normal blood ghrelin level in adults (0.337 ng/ml) was within the linear range of the new system proposed making it suitable for clinical application.

show abstract

“…The authors pointed out that due to its small size and highly parallel measurements, the aptamer-based biochips are much more promising for low-budget protein analysis. Mascini et al [8] reviewed the advancement of aptamer-based assay strategies for diagnostic applications, focusing on the new class of aptamers, i.e. Spiegelmers which composed of Lnucleotides.…”

Section: Introductionmentioning

confidence: 99%

A Review: Electrochemical Aptasensors with Various Detection Strategies

Cheng

et al. 2009

Electroanalysis

120

View full text Add to dashboard Cite

The combinatorial chemical synthesis offers in vitro aptamer generation with limitless aptamer selection for various analytes. Additionally, aptamer has quite many outstanding properties as the biomolecular recognition elements, thus competing with traditional affinity ligands (enzyme, antibodies, lectins etc.) in biological and medicine employments. This article reviews the advancements in electrochemical aptasensors for biomolecule monitoring, emphasizing the efficient detection strategies of sandwich structure formation using two recognition elements to capture and label target respectively, EIS measuring the electronic transfer resistance change, the aptamer conformation change triggering the electrochemical signal on/off and target molecules displacing electroactive markers.

show abstract

Ghrelin Detection Using Spiegelmer‐Capture Molecules

Cited by 19 publications

References 75 publications

Electrochemical Aptasensors

Electrochemical Aptasensors

Colorimetric Microarray Detection System for Ghrelin Using Aptamer‐Technology

A Review: Electrochemical Aptasensors with Various Detection Strategies

Contact Info

Product

Resources

About