Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d’Ivoire: occurrence and evidence for environmental contamination

Berrilli, Federica; D’Alfonso, Rossella; Giangaspero, Annunziata; Marangi, Marianna; Brandonisio, O; Kaboré, Yolande; Glé, Christoph; Cianfanelli, Cristina; Lauro, Renato; Cave, David Di

doi:10.1016/j.trstmh.2011.12.005

Cited by 73 publications

(49 citation statements)

References 29 publications

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“…were identified, as previously reported in African countries [12,17,26]. Regarding parasites, whereas the high rate of G. duodenalis confirms our previous data from Tanzania and Côte d'Ivoire, no patients were detected positive for C. hominis and C. parvum [27][28]. This might be due to the low number of samples tested but also because the xTAG GPP is not able to identify C. meleagridis, a species frequently reported in humans in Africa [29].…”

Section: Discussionsupporting

confidence: 77%

Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Côte d’Ivoire

Cristanziano

Timmen-Wego

Lübke

et al. 2015

J Infect Dev Ctries

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Introduction: Gastrointestinal infections caused by viruses, bacteria, and parasites are endemic in most developing countries due to inadequate provision of safe water supplies, sanitation, and hygiene. To investigate the enteric pathogens infecting people living in Côte d'Ivoire, the Luminex Gastrointestinal Pathogen Panel (xTAG GPP) assay was used to analyze 34 human fecal samples. This study represents the first application of this technology to samples from a sub-Saharan African country. Methodology: Thirty-four stool samples from asymptomatic and symptomatic patients, 1-15 years of age, were analyzed by xTAG GPP. The Luminex assay represents a qualitative bead-based multiplexed molecular diagnostic test able to identify concurrently 15 enteric pathogens, including bacteria, viruses, and parasites. Results: Overall, 22 out of 34 (64.7%) fecal specimens were detected to be positive by xTAG GPP. Sixteen were from asymptomatic subjects, and 10 patients (45.4%) showed co-infections. G. duodenalis was detected in 15 patients, in both mono-and co-infections, representing the most frequent pathogen, followed by enterotoxigenic Escherichia coli (ETEC) LT/ST. Four norovirus isolates were also detected and assigned to genogroups I and II. Conclusions: Considering the burden of enteric infections in developing countries, particularly among children, and the high rate of coinfections in asymptomatic subjects, this study shows the need for diagnostic tools such as xTAG GPP to improve diagnosis and treatment of these infections in endemic areas.

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Section: Discussionsupporting

confidence: 77%

Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Côte d’Ivoire

Cristanziano

Timmen-Wego

Lübke

et al. 2015

J Infect Dev Ctries

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“…A major gap in our understanding of the diversity of cryptosporidia in birds is lack of prevalence and, in particular, genotyping data from the African continent (Bezuidenhout et al 1993, Penrith et al 1994, Soltane et al 2007, Berrilli et al 2012, Baroudi et al 2013. In the present study, we determined the occurrence of Cryptosporidium spp.…”

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confidence: 81%

Cryptosporidium meleagridis and C. baileyi (Apicomplexa) in domestic and wild birds in Algeria

et al. 2017

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A total of 345 faecal samples were collected from domestic, captive and wild birds in rural areas, urban areas and a Zoo in Algeria. Samples were screened for the presence of parasites belonging to the genus Cryptosporidium Tyzzer, 1910 by microscopy and PCR analysis of the small-subunit rRNA (SSU), actin and 60-kDa glycoprotein (gp60) genes. Cryptosporidium spp. were detected in 31 samples. Sequence analysis of SSU and actin genes revealed the presence of C. baileyi Current, Upton et Haynes, 1986 in domestic chicken broilers (n = 12), captive ostriches (n = 4) and a wild mallard (n = 1), and C. meleagridis Slavin, 1955 in a graylag goose (n = 1), chickens (n = 11) and turkeys (n = 2). Twenty-three chicken and two turkey broilers from five farms were positive for cryptosporidia, with an overall prevalence of 2% and 6%, respectively. Both C. meleagridis and C. baileyi were detected in farmed chicken broilers, with a prevalence ranging from 9% to 69%. Farmed turkeys broilers were positive only for C. meleagridis, with a 13% prevalence at the animal level. Subtyping of C. meleagridis isolates at the gp60 locus showed the presence of subtype IIIgA22G3R1 in graylag goose and chicken broilers and IIIgA23G2R1 in chicken and turkey broilers. Infection with cryptosporidia was not associated with any clinical diseases. The results of the present study, which provides the first data on the prevalence of Cryptosporidium spp. in wild birds in Africa, demonstrate the presence of human pathogenic C. meleagridis in both domestic and wild birds in Algeria.

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“…However, Geurden et al (2008) and Berrilli et al (2012) reported that zoonotic genotype A infected goats in Belgium and Côte d´Ivoire, respectively, and Lim et al (2013) reported that genotypes A and B infected goats in Malaysia. These findings suggest that goats could be a potential source of zoonotic infection.…”

Section: Discussionmentioning

confidence: 99%

First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

Sudré¹,

Leles²,

Lima³

et al. 2014

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to perform the first molecular genotyping of Giardia duodenalis from goats in Brazil, in order to assess the risk for zoonotic transmission. Samples were collected from two dairy goat farms (Saanen breed) located in the city of Niteroi in the state of Rio de Janeiro, Brazil. Goat faecal samples (n = 58) were collected directly from the rectums of all animals up to one year of age and were subjected to centrifuge-flotation in sugar saturate solution. Nested-PCR and sequencing using β-giardin and tpi gene targets was performed on positive samples. Seventeen out of fifty-eight (29.31%) faecal samples were positive for Giardia duodenalis cysts, all belonging to the same farm. Only eight isolates were successfully sequenced (eight samples for β-giardin and four samples for tpi), all belonging to genotype E. Two types of sequences were identified for each locus within isolates from the current study, which exhibited sequence heterogeneity with variable numbers of single nucleotide polymorphisms. The present study contributes to a better understanding of the molecular epidemiology of this parasite.

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Giardia duodenalis genotypes and Cryptosporidium species in humans and domestic animals in Côte d’Ivoire: occurrence and evidence for environmental contamination

Cited by 73 publications

References 29 publications

Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Côte d’Ivoire

Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Côte d’Ivoire

Cryptosporidium meleagridis and C. baileyi (Apicomplexa) in domestic and wild birds in Algeria

First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

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