Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Abstract

The present study aimed to produce gibberellic acid through fermentation using banana (Musa sapientum) peel waste as substrate. Banana peel, a domestic and industrial waste, constitutes a potential source of cheap fermentable substrate for the production of other value-added products. Fusarium moniliforme ATCC 10052 and Aspergillus niger CBS 513.88 were used as fermenting organisms. The substrate was dried, ground and its proximate composition determined. The powdered substrate was added to a modified CzapekDox broth (a semisynthetic medium), with Carboxyl methylcellulose (CMC) as control. The fermentation conditions were: pH 5.5; inoculum size 1% (5 × 10 5 spores/mL F. moniliforme) (2 × 10 6 spores/mL A. niger); substrate concentration 2 g; temperature 25 ± 2 o C; fermentation time 7 days. The fermentation was optimized by varying pH, inoculum size, substrate concentration and fermentation time. The extracted GA was subjected to infra-red spectroscopy using FT-IR. The parameters which gave the highest GA yields were thereafter combined in a single fermentation. The results of proximate analysis of banana peel substrate revealed 8.65% moisture, 9.54% protein, 5.40% lipids, 11.45% ash, 22.34% crude fibre, and 42.62% carbohydrate. The GA yields of 13.55 g/L and 12.44 g/L were produced from the banana peel substrate and 3.62 and 2.61 g/L from the CMC control by F. moniliforme and A. niger respectively. Under optimized conditions, F. moniliforme produced 17.48 g/L GA, while A. niger produced 13.50 g/L. Extracted GA was similar to standard GA sample and the present results support the potential use of banana peel for fermentative GA production.

Gibberellic Acid Production from Corn Cob Residues via Fermentation with Aspergillus niger

“…2, the production of GA 3 in the presence of A. niger varied from 2.1 to 6.2 g•kg −1 depending on the fermentation conditions employed during the SSF process (Table 2). As mentioned above, the effects of the pH and sample consistency on GA 3 production were evaluated because pH is one of the most important factors affecting the biomass and yield obtained using A. niger, and the sample consistency is related to the amount of substrate available for GA 3 production [13]. According to Omajasola and Adejoro [13], a balanced amount of substrate is necessary for good GA 3 production.…”

“…As mentioned above, the effects of the pH and sample consistency on GA 3 production were evaluated because pH is one of the most important factors affecting the biomass and yield obtained using A. niger, and the sample consistency is related to the amount of substrate available for GA 3 production [13]. According to Omajasola and Adejoro [13], a balanced amount of substrate is necessary for good GA 3 production. ese authors indicate that GA 3 production and maintenance of biomass require low glucose concentration (<4%) in the culture medium.…”

“…e main fermentation process used to obtain GA 3 is submerged fermentation (SmF); however, the production costs of this process are high owing to low yields and extensive separation steps. In addition, although GA 3 can be isolated from plant tissues and by chemical synthesis, these processes are complicated, give low yields, and are not pro table in an industrial context [13].…”