Gibberellin biosynthesis and gibberellin oxidase activities in Fusarium sacchari, Fusarium konzum and Fusarium subglutinans strains

Segregation of secondary metabolite biosynthesis in hybrids of Fusarium fujikuroi and Fusarium proliferatum

“…After acidification of the culture filtrate, GAs were extracted by partition with EtOAc, purified over C 18 columns and analyzed by full-scan gas chromatography-mass spectrometry (GC-MS) after derivatization as described (Troncoso et al, 2010). GA 3 :GA 1 ratios were calculated from the areas of the respective total ion current peaks.…”

“…1). Beside F. fujikuroi, only a few isolates of three other species in the GFC, F. konzum, F. sacchari and F. proliferatum, are known to produce GAs (Malonek et al, 2005a(Malonek et al, , 2005b(Malonek et al, , 2005cTsavkelova et al, 2008;Troncoso et al, 2010).…”

Growth promotion mediated by endophytic fungi in cloned seedlings of Eucalyptus grandis x Eucalyptus urophylla hybrids

“…Many strains of the genus Fusarium and other endophytic fungi can produce phytohormones (Nassar et al, 2005) that directly influence plant development. It is well established that some Fusarium strains are capable of producing the plant hormone gibberellin (GA) (Troncoso et al, 2010;Tudzynski, 2005). Working with strains of Fusarium proliferatum that are symbiotic with orchids, Tsavkelova et al (2008) identified an isolate that is a significant producer of GAs, and strains symbiotic with Physalis alkekengi have been shown to synthesize a wide range of GAs, including GA 3 (Rim et al, 2005).…”

Genome Mining for Fungal Secondary Metabolic Gene Clusters

“…Ascomycota on the other hand are known to be prolific producers of bioactive diterpenoids and several biosynthetic gene clusters have been characterized. These include the well-studied gibberellin pathways found in several fungi that use a bifunctional diterpene synthase that combines the domains of a class I and class II terpene synthases (which are separate enzymes in plant diterpene biosynthesis) to cyclize GGPP [111][112][113]. Mining of Ascomycota genomes followed by gene deletion studies and stepwise heterologous coexpression of pathway genes in Aspergillus has led to the elucidation of additional gene clusters involved in the biosynthesis of the diterpene compounds fusicoccin, brassicicene, aphidicolin and phomopsene [54,55,[114][115][116][117][118][119][120], and a sesterterppenoid (C 25 ) [121].…”