Giemsa C-banding of potato chromosomes

Abstract

An air-drying technique was developed that provided well-spread somatic metaphases of the potato Solanum tuberosum. With Giemsa C-banding, the individual chromosomes (x = 12) could be identified. Some bands are different from those in S. phureja. The technique provides a means to investigate structural differences between genomes.Key words: C-banding, chromosomes, potato.

Chromosome Identification in Potato Trisomics(2n=2x+1=25) by Conventional Staining, Giemsa C-banding and Non-radioactive in situ Hybridization.

“…Variation for the length of the NORs of homologues or homoeologues of chromosome 2 as well as the presence of a satellite on this chromosome was found in various cytotypes in this study. The nucleolar organizer chromosomes from two clones, designated IVP35 and IVP48 from S. phureja, were not recognizable using the C-banding technique (Pijnacker and Ferwerda 1984). The absence of a satellite on chromosome 2 (NOR was visible) in one of the Gineke monohaploids studied suggests that one of the four nucleolar chromosomes of Gineke is an unsatellited chromosome.…”

“…6f, 7f). It is however not certain whether these chromosomes are identical to chromosome 6 described by Pijnacker and Ferwerda (1984) . Two unsatellited chromosomes 2 are shown in Fig.…”

“…Giemsa C-banding of chromosomes Table 2 presents the results of the Giemsa C-banding of chromosomes from intraspecific and interspecific trisomics. The chromosomes of the two intraspecific trisomics have been arranged according to their length and specific banding pattern as described by Pijnacker and Ferwerda (1984). Because the banding pattern was unsatisfactory, a proper identification of all chromosomes was impossible.…”

“…Another constraint of this technique is that the centromeres of prophase chromosomes are not visible. Later on Pijnacker and Ferwerda (1984) developed a Giemsa C-banding technique for the identification of somatic metaphase chromosomes.…”

Repeated DNA and heterochromatin structure in Rumex acetosa

“…C-banding C-banding was performed by methods including those of Linde-Laursen (1975), Schwarzacher et al (1980), Pijnacker & Ferwerda (1984) and Hutchinson, J.…”