Giemsa Staining of Mitotic Chromosomes in Kluyveromyces lactis and Saccharomyces cerevisiae

Galeotti, Cesira L.; Williams, Keith L.

doi:10.1099/00221287-104-2-337

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…However, neither TRPl sequence is flanked by significant direct repeat sequences suggestive of transposition events. The chromosomal organization of the two yeasts is known to be different, with K. lactis having approximately nine chromosomes (Galeotti and Williams, 1978) compared with 17 in S . cerevisiae.…”

Section: The K Lactis Ipp Genementioning

confidence: 99%

Cloning and analysis of the Kluyveromyces lactis TRP1 gene: A chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3

Stark

Milner

1989

Yeast

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The TRP1 gene of the yeast Kluyveromyces lactis has been cloned from a genomic library by complementation of the Saccharomyces cerevisiae trp1-289 mutation. The gene was located within the clone by transposon mutagenesis and the coding region identified by DNA sequencing. This has indicated that K. lactis TRP1 encodes a 210-amino acid polypeptide which shows 53% identity to the homologous S. cerevisiae protein. The K. lactis TRP1 gene has been disrupted by substituting the S. cerevisiae URA3 gene for a large part of the TRP1 coding sequence. Replacement of the chromosomal TRP1 locus with this construction has enabled the production of non-reverting trp1- strains of K. lactis, while a genetic analysis of the disrupted allele confirmed that the TRP1 gene had been cloned. DNA sequencing has also shown that the K. lactis TRP1 sequence is flanked by genes encoding inorganic pyrophosphatase and histone H3, which we have designated IPP and HHT1 respectively. Hybridization studies have shown that in common with S. cerevisiae, K. lactis has two copies of the histone H3 gene. Each H3 gene is closely linked to a gene encoding histone H4 and in both yeast species the IPP gene is tightly linked to one of the histone gene pairs.

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Section: The K Lactis Ipp Genementioning

confidence: 99%

Cloning and analysis of the Kluyveromyces lactis TRP1 gene: A chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3

Stark

Milner

1989

Yeast

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“…This study Galeotti and Williams (1978), Whittacker and Leach (1978) This study This study Gygax and Thuriaux (1984), Erard and Barker (1985), Bostock (1970) This study This study Bachmann and Brooks Low (1980) Perkins and Barry (1977) DuPraw (1 970)…”

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confidence: 93%

Orthogonal‐field‐alternation gel electrophoresis banding patterns of DNA from yeasts

Jonge

Jongh

Meijers

et al. 1986

Yeast

153

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Chromosomal DNAs from various yeast species were separated by orthogonal-field-alternation gel electrophoresis (OFAGE). To this end we developed a spheroplasting and lysis method to obtain intact DNA from both ascomycetous and basidiomycetous yeasts. The OFAGE banding patterns of22 ascomycetous and four basidiomycetous yeast strains were compared. The strains represented species from the genera: Brettanomyces, Candida, Cryptococcus, Filohasidiella, Geotrichum, Hansenula, Kluyveromyces, Pachysolen, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Saccharomycodes, Saccharomycopsis, Schizosaccharomyces and Zygosaccharomyces. Variations occurred in the number of bands and their positions in the gel, not only among strains of different genera but also among species from the same genus and even between varieties of the same species. The ascomycetous yeasts, with the exception of Saccharomyces cerevisiae, only showed one to five bands of DNA larger than 1000 kilobase pairs (kb) in general none smaller. The patterns of the four basidiomycetous yeasts revealed also a few large DNA bands but in addition one to six bands ranging in size from 500 to 1000 kb, with the exception of a single smaller chromosome in Rhodotorula mucilaginosa. From the OFAGE banding patterns of strains studied here it appears that in Sacch. cerevisiae the partitioning of DNA over chromosomes is unique. But rather than the large number of chromosomes, the presence of four chromosomes with less than 500 kb of DNA is characteristic for Sacch. cerevisiae.

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“…However, imperfect yeasts cannot be studied using traditional genetics. Another approach to obtain direct evidence for chromosome numbers is cytochemical staining of mitotic chromosomes with Giemsa, e.g., in Kluyveromyces lactis and Saccharomyces cerevisiae (Galeotti & Williams 1978), Lipomyces lipofer (Robinow 1961), andSchizosaccharomyces pombe (Fisher et al 1975). DNA-binding fluorochromes like DAPI, mithramicin and para-rosaniline do not reveal distinct chromosomal patterns at the light microscopic level.…”

Section: Introductionmentioning

confidence: 99%

The use of karyotyping in the systematics of yeasts

Boekhout¹,

Renting

Scheffers

et al. 1993

Antonie van Leeuwenhoek

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The use of electrophoretic karyotyping in systematics of yeasts is discussed. New data are provided on the karyotypes of the medically important fungi Hortaea werneckii, Filobasidiella (= Cryptococcus) neoformans, and Malassezia species. Hortaea werneckii has twelve to eighteen bands of chromosomal DNA, ranging in size between 500 and 2300 kb. The karyotypes of Filobasidiella neoformans consist of seven to fourteen bands of chromosomal DNA. The varieties neoformans and bacillispora cannot be separated by their karyotypes, and no obvious correlation was found with serotypes, geography or habitat. All strains of Malasseziapachydermatis studied have similar karyotypes consisting of five bands, whereas in M. furfur, four different karyotypes are prevalent. However, each of these karyotypes is stable.

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Giemsa Staining of Mitotic Chromosomes in Kluyveromyces lactis and Saccharomyces cerevisiae

Cited by 11 publications

References 11 publications

Cloning and analysis of the Kluyveromyces lactis TRP1 gene: A chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3

Cloning and analysis of the Kluyveromyces lactis TRP1 gene: A chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3

Orthogonal‐field‐alternation gel electrophoresis banding patterns of DNA from yeasts

The use of karyotyping in the systematics of yeasts

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