Gingival crevicular fluid collagenase‐2 (MMP‐8) test stick for chair‐side monitoring of periodontitis

Mäntylä, Päivi; Stenman, Mathias; Kinane, Denis F.; Tikanoja, S; Luoto, Hanne; Salo, Tuula; Sorsa, Timo

doi:10.1034/j.1600-0765.2003.00677.x

Cited by 196 publications

(262 citation statements)

References 16 publications

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“…Chlorhexidine used as a adjunctive medication in the periapical treatment in addition to its antimicrobial properties, exert properties directly to inhibit the MMPs and their oxidative activation. [71][72][73] …”

Section: Role Of Mmps In Periapical Inflammationmentioning

confidence: 99%

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

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Section: Role Of Mmps In Periapical Inflammationmentioning

confidence: 99%

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

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“…Data reported for MMP-8 using immunofluorometric assays suggested a lower limit of detection of approximately 1 ng/mL. 1,18 In multiplex bead immunoassay, the sensitivity of the assay for MMP-8 was 0.03 ng/mL, and 90% of GCF samples had MMP levels above 7.2 ng/mL (0.36 ng/site). Therefore, the sensitivity of the multiplex bead immunoassay used in our study seems to be adequate to detect even small differences in GCF levels of MMPs.…”

Section: Discussionmentioning

confidence: 99%

“…In previous articles 1,18 in which increases in GCF levels of MMP-8 were detected as a result of the application of orthodontic forces to periodontally healthy teeth, the levels detected were still below those found in gingivitis and periodontitis. This indicates that elevations of GCF MMP-8 were likely still compatible with periodontal health.…”

Section: Discussionmentioning

confidence: 99%

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Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Almeida

Capelli

Teles³

2015

The Angle Orthodontist

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Objective: To examine levels of matrix metalloproteinases (MMPs)-1, -2, -3, -7, -8, -12, and -13 in the gingival crevicular fluid (GCF) of periodontally compromised teeth at different time points during orthodontic movement. Materials and Methods: Ten controlled periodontitis subjects were submitted to orthodontic treatment. One dental arch was subjected to orthodontic movement, and teeth in the opposite arch were used as controls. GCF samples were collected from the lingual sites of two movement and two control incisors 1 week before orthodontic activation (27 d), immediately after orthodontic activation, and after 1 hour, 24 hours, and 7, 14, and 21 days. Multiplexed bead immunoassay was used to measure MMPs in GCF. Data were analyzed using Friedman and Wilcoxon statistical tests. Results: The only significant change found over time was in the levels of MMP-1 in the movement group (P , .05). When the two groups were compared after activation, the only statistically significant difference found was in levels of MMP-12 24 hours after activation (P , .05). Conclusions: Our findings suggested that the orthodontic movement of periodontally compromised teeth without active pockets did not result in significant changes in the GCF levels of MMPs.

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“…2,[11][12][13][14][15][16] A time resolved immunofluorometric assay (IFMA), ELISA 그리고 a dentoAnalyzer device 등을 이용해서 MMP-8을 분석해 본 결과 방법에 상관없이 일치하는 결과 를 보였다는 연구도 있었지만, 16 ELISA의 경우 다른 방법 들과는 다른 종류의 antibody를 사용하게 되어서 조금 다 른 결과를 보일 수도 있다고 보고되기도 했다. 17 치주영역에 서는 치은 열구액을 채취하여 chair-side에서 MMP-8의 농도를 측정하여 진단에 이용할 수 있는 기구가 개발되어 있다.…”

Section: 또한 같은 샘플을 희석하여 Mmp-8과 Sp의 분석에 동시에 사용할 수 있는 이점이 있었다unclassified

Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

Shin

Lee²,

Lee

et al. 2011

J Korean Acad Conserv Dent

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Objectives:The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth. Materials and Methods: Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05). Results: MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level. Conclusions: This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis. [J Kor Acad Cons Dent 2011;36(3):196-202.]

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Gingival crevicular fluid collagenase‐2 (MMP‐8) test stick for chair‐side monitoring of periodontitis

Cited by 196 publications

References 16 publications

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

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