Gingival crevicular fluid levels of cytokines/chemokines in chronic periodontitis: a meta‐analysis

Stadler, Amanda Finger; Angst, Patrícia Daniela Melchiors; Arce, Roger M.; Gomes, Sabrina Carvalho; Oppermann, Rui Vicente; Susin, Cristiano

doi:10.1111/jcpe.12557

Cited by 180 publications

(160 citation statements)

References 99 publications

Supporting

Mentioning

140

Contrasting

Unclassified

Order By: Relevance

“…IL-6 level in gingival crevicular fluid showed significant positive correlation with the clinical parameters in periodontitis [21, 24]. Meanwhile, Mahanonda et al mentioned that IL-6 was involved in local B cell responses and was detected in inflamed gingival tissues [19].…”

Section: Discussionmentioning

confidence: 99%

miR-146a regulates inflammatory cytokine production in Porphyromonas gingivalis lipopolysaccharide-stimulated B cells by targeting IRAK1 but not TRAF6

Jiang

Deng

et al. 2018

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

View full text Add to dashboard Cite

It has been suggested that microRNAs (miRs) are involved in the immune regulation of periodontitis. However, it is unclear whether and how miRs regulate the function of B cells in the context of periodontitis. This study is to explore the role of miR-146a on the inflammatory cytokine production of B cells challenged by Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS). Primary B cells were harvested from mouse spleen. Quantitative real-time polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of inflammatory cytokines in B cells in the presence or absence of P. gingivalis LPS and/or miR-146a. Bioinformatics, luciferase reporter assay and overexpression assay were used to explore the binding target of miR-146a. Our results showed that miR-146a level in B cells was elevated by P. gingivalis LPS stimulation, and the mRNA expressions of interleukin (IL)-1β, 6 and 10, and IL-1 receptor associated kinase-1 (IRAK1), but not TNF receptor associated factor 6 (TRAF6), were also upregulated. The expression levels of IL-1β, 6, 10 and IRAK1 were reduced in the presence of miR-146a mimic, but were elevated by the addition of miR-146a inhibitor. MiR-146a could bind with IRAK1 3' untranslated region (UTR) but not TRAF6 3'-UTR. Overexpression of IRAK1 reversed the inhibitory effects of miR-146a on IL-1β, 6 and 10. In summary, miR-146a inhibits inflammatory cytokine production in B cells through directly targeting IRAK1, suggesting a regulatory role of miR-146a in B cell-mediated periodontal inflammation.

show abstract

Section: Discussionmentioning

confidence: 99%

miR-146a regulates inflammatory cytokine production in Porphyromonas gingivalis lipopolysaccharide-stimulated B cells by targeting IRAK1 but not TRAF6

Jiang

Deng

et al. 2018

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

View full text Add to dashboard Cite

show abstract

“…Likewise, chemokines are small signaling molecules responsible for the regulated trafficking of specific cell types into the inflamed tissues [57]. The balance and relative abundance of chemokines and cytokines is the ultimate responsible for the tissue changes during the progression of periodontitis and the tilting of their balance could determine the duration, intensity, and character of the response [58,59]. Depending on the relative abundance and kinetics of the expression of these molecular signals the response to periodontal infection can range from subclinical inflammation to destructive periodontitis [60].…”

Section: Mmp-mediated Regulation Of Inflammatory Responsementioning

confidence: 99%

Matrix Metalloproteinases as Regulators of Periodontal Inflammation

Cavalla

Patricia

Sorsa

et al. 2017

IJMS

237

196

View full text Add to dashboard Cite

Periodontitis are infectious diseases characterized by immune-mediated destruction of periodontal supporting tissues and tooth loss. Matrix metalloproteinases (MMPs) are key proteases involved in destructive periodontal diseases. The study and interest in MMP has been fuelled by emerging evidence demonstrating the broad spectrum of molecules that can be cleaved by them and the myriad of biological processes that they can potentially regulate. The huge complexity of MMP functions within the ‘protease web’ is crucial for many physiologic and pathologic processes, including immunity, inflammation, bone resorption, and wound healing. Evidence points out that MMPs assemble in activation cascades and besides their classical extracellular matrix substrates, they cleave several signalling molecules—such as cytokines, chemokines, and growth factors, among others—regulating their biological functions and/or bioavailability during periodontal diseases. In this review, we provide an overview of emerging evidence of MMPs as regulators of periodontal inflammation.

show abstract

“…Interleukin (IL)‐1β is one of the most important biomarkers in this context. As recently summarized in a systematic review, the level of IL‐1β in gingival crevicular fluid is significantly higher in patients diagnosed with chronic periodontitis than in periodontally healthy individuals . Moreover, the level of IL‐1β in gingival crevicular fluid has been shown to decrease after nonsurgical periodontal treatment …”

Section: Introductionmentioning

confidence: 98%

Salivary, gingival crevicular fluid and serum levels of ghrelin and chemerin in patients with periodontitis and overweight

Jentsch

Arnold

Richter

et al. 2017

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

Gingival crevicular fluid levels of cytokines/chemokines in chronic periodontitis: a meta‐analysis

Abstract: Evidence for significant differences between periodontal health and ChP was observed for a few cytokines and one chemokine. No conclusions could be drawn with regards to increased risk of disease progression.

Cited by 180 publications

References 99 publications

miR-146a regulates inflammatory cytokine production in Porphyromonas gingivalis lipopolysaccharide-stimulated B cells by targeting IRAK1 but not TRAF6

miR-146a regulates inflammatory cytokine production in Porphyromonas gingivalis lipopolysaccharide-stimulated B cells by targeting IRAK1 but not TRAF6

Matrix Metalloproteinases as Regulators of Periodontal Inflammation

Salivary, gingival crevicular fluid and serum levels of ghrelin and chemerin in patients with periodontitis and overweight

Contact Info

Product

Resources

About