Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease

Zhong, Wei Long; Peng, Youmei; Yue, Erli; Huang, Bin; Zhang, Wei; Zhao, Zhe; Jiang, Jinhua; Wang, Qingduan; Zhao, Hongyu

doi:10.1111/odi.13227

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…The detection rate of A. actinomycetemcomitans in patients with periodontitis was consistent with previous reports [ 45 , 46 ]. However, probably due to different sampling or processing methods, the detection rate of other periodontal pathogens in GCF at baseline and 6 months after therapy was lower compared with previous studies [ 30 , 47 ].…”

Section: Discussionmentioning

confidence: 59%

Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis

Ma,

Liu,

et al. 2024

BMC Oral Health

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Background Serving as a stop signal of inflammation, the role of lipoxin A4 (LXA4) in periodontitis remains to be clarified. This study is aimed to examine the changes in LXA4 levels in gingival crevicular fluid (GCF) after scaling and root planing (SRP) and to determine the relationship between LXA4 levels and treatment outcomes and periodontal pathogens in severe periodontitis. Methods A total of 74 GCF samples were collected from 21 severe periodontitis participants at the deepest affected sites. These sites were re-sampled at 1, 3, and 6 months after SRP. Besides, GCF samples were also collected from 25 periodontally healthy participants. Clinical parameters including probing depth (PD) and clinical attachment level (CAL) in periodontitis group were recorded. LXA4 levels and periodontal pathogens in the GCF were analyzed by ELISA and PCR, respectively. Correlations between GCF LXA4 levels and treatment effect and periodontal pathogens were assessed. Results LXA4 levels in GCF significantly increased after SRP (p < 0.05), but remained lower than those observed in healthy individuals (p < 0.05). Sites with lower baseline LXA4 concentrations were more likely to experience greater improvements in PD at 6 months post-SRP (area under the curve [AUC] = 0.792), and the improvements were positively correlated with the increase of LXA4 at these sites post-treatment (p < 0.05). Furthermore, more elevated LXA4 levels were observed in sites that became negative for Prevotella intermedia or Tannerella forsythia after SRP. Conclusion Baseline LXA4 in GCF has the potential to predict the site-specific response of severe periodontal lesions to SRP. The increase of LXA4 levels after treatment was positively correlated with clinical improvements and negatively correlated with the presence of Prevotella intermedia or Tannerella forsythia.

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Section: Discussionmentioning

confidence: 59%

Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis

Ma,

Liu,

et al. 2024

BMC Oral Health

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“…SLIT3 could positively affect type H angiogenesis through the SLIT-ROBO receptor (Xu et al 2018). In the field of periodontology, only 1 study detected SLIT3 in gingival crevicular fluid (Zhong et al 2020). In our study, SLIT3 was first validated to be expressed in the PDL and reduced in the absence of mechanical loading.…”

Section: Discussionmentioning

confidence: 99%

Occlusal Force Maintains Alveolar Bone Homeostasis via Type H Angiogenesis

Chen,

Yin,

Luo

et al. 2023

J Dent Res

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Physiologically, teeth and periodontal tissues are exposed to occlusal forces throughout their lifetime. Following occlusal unloading, unbalanced bone remodeling manifests as a net alveolar bone (AB) loss. This phenomenon is termed alveolar bone disuse osteoporosis (ABDO), the underlying mechanism of which remains unclear. Type H vessels, a novel capillary subtype tightly coupled with osteogenesis, reportedly have a role in skeletal remodeling; however, their role in ABDO is not well studied. In the present study, we aimed to explore the pathogenesis of and therapies for ABDO. The study revealed that type H endothelium highly positive for CD31 and endomucin was identified in the periodontal ligament (PDL) but rarely in the AB of the mice. In hypofunctional PDL, the density of type H vasculature and coupled osterix+ (OSX+) osteoprogenitors declined significantly. In addition, the angiogenic factor Slit guidance ligand 3 (SLIT3) was downregulated in the disused PDL, and periodontal injection of the recombinant SLIT3 protein partially ameliorated type H vessel dysfunction and AB loss in ABDO mice. With regard to the molecular mechanism, a mechanosensory signaling circuit, PIEZO1/Ca2+/HIF-1α/SLIT3, was validated by applying cyclic compression to 3-dimensional–cultured PDL cells using the Flexcell FX-5000 compression system. In summary, PDL plays a pivotal role in mechanotransduction by translating physical forces into the intracellular signaling axis PIEZO1/Ca2+/HIF-1α/SLIT3, which promotes type H angiogenesis and OSX+ cell–related osteogenensis, thereby contributing to AB homeostasis. Our findings advance the understanding of PDL in AB disorders. Further therapies targeting SLIT3 may provide new insights into preventing bone loss in ABDO.

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“…In addition, macrophages secrete increased proinflammatory cytokines such as TNF-a, interferon-g (IFN-g), IL-1a, IL-1b, IL-6, and IL-12; increased adhesion factors such as CXCL5 and CXCL1 (54,59,60); and increased inflammatory bodies such as NLRP3. In addition, the expression of other molecules such as toll-like receptors 2 (TLR2), TLR4, and nucleotide-binding oligomerization domain 2 (NOD2) is also increased (61), mainly by in vivo cytokines such as IL-17 and pathogenic bacteria such as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) (62,63).…”

Section: Drugs Targeting Macrophagesmentioning

confidence: 99%

Immunomodulation in the Treatment of Periodontitis: Progress and Perspectives

et al. 2021

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Periodontitis is one of the most common dental diseases. Compared with healthy periodontal tissues, the immune microenvironment plays the key role in periodontitis by allowing the invasion of pathogens. It is possible that modulating the immune microenvironment can supplement traditional treatments and may even promote periodontal regeneration by using stem cells, bacteria, etc. New anti-inflammatory therapies can enhance the generation of a viable local immune microenvironment and promote cell homing and tissue formation, thereby achieving higher levels of immune regulation and tissue repair. We screened recent studies to summarize the advances of the immunomodulatory treatments for periodontitis in the aspects of drug therapy, microbial therapy, stem cell therapy, gene therapy and other therapies. In addition, we included the changes of immune cells and cytokines in the immune microenvironment of periodontitis in the section of drug therapy so as to make it clearer how the treatments took effects accordingly. In the future, more research needs to be done to improve immunotherapy methods and understand the risks and long-term efficacy of these methods in periodontitis.

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Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease

Cited by 6 publications

References 38 publications

Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis

Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis

Occlusal Force Maintains Alveolar Bone Homeostasis via Type H Angiogenesis

Immunomodulation in the Treatment of Periodontitis: Progress and Perspectives

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