Gingival, Dermal, and Periodontal Ligament Fibroblasts Express Different Extracellular Matrix Receptors

Palaiologou, Archontia; Yukna, Raymond A.; Moses, R. L.; Lallier, Thomas E.

doi:10.1902/jop.2001.72.6.798

Cited by 97 publications

(105 citation statements)

References 48 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…We wished to investigate the potential role of selected integrins in mediating CCN2/CTGF stimulated collagen deposition by gingival fibroblasts. Gingival fibroblasts express several integrins, including all of those investigated in the present study [Palaiologou et al, 2001;van der Pauw et al, 2002]. The collagen deposition assay was therefore performed in the presence of integrin neutralizing antibodies.…”

Section: Integrin Antibody Inhibition Studiesmentioning

confidence: 99%

CCN2, connective tissue growth factor, stimulates collagen deposition by gingival fibroblasts via module 3 and α6‐ and β1 integrins

Heng

Huang

Black

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

CCN2, (connective tissue growth factor, CTGF) is a matricellular factor associated with fibrosis that plays an important role in the production and maintenance of fibrotic lesions. Increased collagen deposition and accumulation is a common feature of fibrotic tissues. The mechanisms by which CCN2/CTGF contributes to fibrosis are not well understood. Previous studies suggest that CTGF exerts some of its biological effects at least in part by integrin binding, though this mechanism has not been previously shown to contribute to fibrosis. Utilizing full length CCN2/CTGF, CCN2/CTGF fragments, and integrin neutralizing antibodies, we provide evidence that the effects of CCN2/CTGF to stimulate extracellular matrix deposition by gingival fibroblasts are mediated by the C-terminal half of CCN2/CTGF, and by alpha6 and beta1 integrins. In addition, a synthetic peptide corresponding to a region of CCN2/CTGF domain 3 that binds alpha6beta1 inhibits the collagen-deposition assay. These studies employed a new and relatively rapid assay for CCN2/CTGF-stimulated collagen deposition based on Sirius Red staining of cell layers. Data obtained support a pathway in which CCN2/CTGF could bind to alpha6beta1 integrin and stimulate collagen deposition. These findings provide new experimental methodologies applicable to uncovering the mechanism and signal transduction pathways of CCN2/CTGF-mediated collagen deposition, and may provide insights into potential therapeutic strategies to treat gingival fibrosis and other fibrotic conditions.

show abstract

Section: Integrin Antibody Inhibition Studiesmentioning

confidence: 99%

CCN2, connective tissue growth factor, stimulates collagen deposition by gingival fibroblasts via module 3 and α6‐ and β1 integrins

Heng

Huang

Black

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Differential regulation of these two MMP genes is likely to be of biological importance, and may be related to the generally lower tendency for scar formation in oral tissues (Ravanti et al, 1999). In addition, integrin expression patterns differ between gingival fibroblasts and dermal fibroblasts, whereas periodontal ligament fibroblasts and gingival fibroblasts are more similar to each other in this respect (Palaiologou et al, 2001). It is interesting to note that human gingival fibroblasts and periodontal ligament fibroblasts express a variety of different genes differentially, including members of the CCN family of genes (Han and Amar, 2002).…”

Section: Unique Aspects Of Gingival Fibroblast Metabolismmentioning

confidence: 99%

Connective Tissue Metabolism and Gingival Overgrowth

Trackman

Kantarcı

2004

Critical Reviews in Oral Biology & Medicine

136

152

View full text Add to dashboard Cite

Gingival overgrowth occurs mainly as a result of certain anti-seizure, immunosuppressive, or antihypertensive drug therapies. Excess gingival tissues impede oral function and are disfiguring. Effective oral hygiene is compromised in the presence of gingival overgrowth, and it is now recognized that this may have negative implications for the systemic health of affected patients. Recent studies indicate that cytokine balances are abnormal in drug-induced forms of gingival overgrowth. Data supporting molecular and cellular characteristics that distinguish different forms of gingival overgrowth are summarized, and aspects of gingival fibroblast extracellular matrix metabolism that are unique to gingival tissues and cells are reviewed. Abnormal cytokine balances derived principally from lymphocytes and macrophages, and unique aspects of gingival extracellular matrix metabolism, are elements of a working model presented to facilitate our gaining a better understanding of mechanisms and of the tissue specificity of gingival overgrowth.

show abstract

“…The reductions in levels of syndecans-2 and -4 and fibroblast growth factor receptor 1 may be involved in the control of the growth and differentiation of periodontal ligament cells during development and regeneration (Shimazu et al, 1999). Palaiologou et al (2001) have elucidated the extracellular matrix receptors expressed in gingival, dermal, and periodontal ligament fibroblasts, finding that these three fibroblast-types differ in the way they behave and express different extracellular matrix receptors. However, gingival fibroblasts and periodontal ligament fibroblasts are more similar in their attachment and expression of integrin than either is to dermal fibroblasts.…”

Section: Syndecanmentioning

confidence: 99%

Regulatory mechanisms of periodontal regeneration

Shimono¹,

Ishikawa²,

Ishikawa³

et al. 2003

Microscopy Res & Technique

202

162

View full text Add to dashboard Cite

The periodontal ligament, located between the cementum and the alveolar bone, has a width ranging from 0.15 to 0.38 mm. Regeneration and homeostasis of the periodontal ligament are highly significant functions in relation to periodontal therapy, tooth transplantation or replantation, and orthodontic tooth movement. The purpose of this review is to discuss the regulatory mechanisms of regenerative and homeostatic functions in the periodontal ligament based on currently published studies and also on our own experimental data. We consider the capability of the ligament tissue to promote or to suppress calcification in connection with bone and cementum formation and the maintenance of the periodontal ligament space. Also discussed are the involvement of the periodontal ligament tissue in the regenerative ability, cell proliferation, growth and differentiation factors, extracellular matrix proteins, homeostatic phenomena, function of Malassez epithelial rests, tooth movement, or occlusal loading. Regulatory mechanisms for regeneration and homeostasis of the periodontal ligament are hypothetically proposed.

show abstract

Gingival, Dermal, and Periodontal Ligament Fibroblasts Express Different Extracellular Matrix Receptors

Cited by 97 publications

References 48 publications

CCN2, connective tissue growth factor, stimulates collagen deposition by gingival fibroblasts via module 3 and α6‐ and β1 integrins

CCN2, connective tissue growth factor, stimulates collagen deposition by gingival fibroblasts via module 3 and α6‐ and β1 integrins

Connective Tissue Metabolism and Gingival Overgrowth

Regulatory mechanisms of periodontal regeneration

Contact Info

Product

Resources

About