Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide

Oyama, Yasuo; Chikahisa, Lumi; Ueha, Toshiko; Kanemaru, Kaori; Noda, Kazuhiro

doi:10.1016/0006-8993(95)01440-3

Cited by 181 publications

(103 citation statements)

References 13 publications

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“…[9][10][11] Based upon their antioxidant activity, flavonoids were recently shown to actively prevent and treat various diseases such as cancer, ischemic injury of the heart and brain, and AIDS. [12][13][14][15] After mice were fed extracts of Physalis angulata flower and Rosa hybrida flower for 3 weeks, the activities of superoxide dismutase and catalase were increased and lipid peroxidation was inhibited in blood serum. Specifically, the concentration of HDL cholesterol was increased in the blood serum of mice fed extract of Rosa hybrida flower.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity ofRubuscoreanus Fruit Extract: In Comparison to Green Tea Extract

et al. 2010

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This study aimed to evaluate the antioxidant activity of Korean raspberry (Rubus coreanus Miq.) fruits, which are grown widely in the southwest area of Korea. Freeze-dried Rubus coreanus (RC) fruits and green tea (GT) were used to obtain water extracts (RCE and GTE, respectively). The total phenolic contents of the dried RC and GT were 126μmol CE (catechin equivalent)/g and 360μmol CE/g, respectively. The free radical scavenging activity of RCE and GTE was expressed as SC50 (50% scavenging capacity) and compared. The scavenging activity of RC toward DPPH, superoxide, and hydroxyl radicals was lower than that of GT. The inhibitory activity of RCE and GTE on AAPH-induced lipid peroxidation was also analyzed. In contrast with the free radical scavenging activities, the lipid peroxidation-inhibiting activity of RC was superior to that of GT. These results suggest that RCE contains effective scavengers of peroxyl radicals. In summary, RC contains less phenolics than does GT. Although RC has lower activity for scavenging DPPH, superoxide, and hydroxyl radicals than does GT, its activity to inhibit peroxyl radical-lipid peroxidation was higher. These results suggest that RC could be used as a valuable natural source of antioxidants. Materials and Methods ChemicalsAAPH (3,2-azobis (2-amidinopropane) dihydrochloride), PMS (phenazine methosulfate), NBT (nitroblue tetrazolium), TBA (thiobarbituric acid), TCA (trichloroacetic acid), and MDA (malondialdehyde) were purchased from Sigma (St. Louis, MO, USA). CCA (coumarin-3-carboxylic acid) was purchased from Fluka (Buchs, Switzerland). High-grade GT was purchased at a store. Preparation of water extracts from Rubus coreanusfruits and green tea RC fruits were collected from Gochang, Korea.Aqueous extracts of RC and GT were prepared by the following method. RC fruits were frozen overnight at −80 o C and then dried by using a lyophilizer (Hanil Science Industrial Co., Incheon, Korea). Dried fruits (5 g) and dried green tea (5 g, Hankook Tea Co., Gwangju, Korea) were homogenized in distilled water Quantitation of phenol groupsThe phenol content of RCE and GTE was measured with FCR. 25 Aliquots of diluted RCE (200μL) and GTE (200μL) were mixed with 10% FCR (1 mL) in test tubes. The mixture was left to react for 3 min at room temperature, followed by the addition of 7.5% Na 2 CO 3 (0.8 mL) and incubation for 2 h in a shaking water bath.After incubation, the absorbance of the mixture was measured at 760 nm by use of a UV-spectrophotometer (model UV-1650PC, Shimadzu, Japan). The phenolic content was quantified from the standard curve plotted

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Section: Introductionmentioning

confidence: 99%

Antioxidant Activity ofRubuscoreanus Fruit Extract: In Comparison to Green Tea Extract

et al. 2010

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“…Extensive studies on G. biloba extracts showed their ability to protect brain neurons from oxidative stress 19 and to inhibit apoptosis in cell culture. 20 The G. biloba extracts that are currently used for medicinal purposes contain 24% flavonoid glycosides (quercetin, kaempferol, isorhamnetin) and 6% terpene lactones (ginkgolides A, B, C, M, J and bilobalides).…”

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confidence: 99%

“…51 Several studies have shown that EGb 761 could protect cultured neurons against damage induced by peroxynitrate and hydrogen peroxide. 19,51 Zhang et al demonstrated that total ginkgolides (TG) (a terpenoid constituent of EGb 761) protected cultured rat cortical neurons from oxidative damages induced by hydrogen peroxide (H 2 O 2 ). 52 EGb 761 extract, with free radical scavenging activity, has been shown to reduce the size of cerebral infarction and improve neurological behaviour in rats with permanent and transient mid-cerebral artery occlusion (MCAo).…”

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confidence: 99%

Oxidative Stress and C-Reactive Protein in Patients with Cerebrovascular Accident ( Ischaemic Stroke ) : The Role of Ginkgo Biloba Extract

Thanoon¹,

Abdul-Jabbar²,

Taha³

2012

SQUMJ

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abstract:Objectives: This study aimed to investigate the presence of oxidative stress and inflammation in ischaemic stroke patients by measuring malondialdehyde (MDA), total antioxidant status (TAS), and highlysensitivity C-reactive protein (hsCRP) in the early post-ischaemic period, and to determine the role of Ginkgo biloba therapy in correcting the markers of oxidative stress and inflammation. Methods: This study was conducted at Ibn Seena Hospital, Mosul City, Iraq and included 31 cerebrovascular accident (CVA) patients and 30 healthy controls. Ischaemic stroke patients were divided into two groups: group I (n = 15) received conventional therapy; group II (n = 16) received conventional therapy with G. biloba (1500 mg/day) for 30 days. Blood samples were obtained from patients and controls before treatment and assays done of serum levels of MDA, TAS, and hsCRP. For CVA patients, a post-treatment blood sample was taken and the same parameters reassessed. Results: Compared with the controls, patients' serum levels of MDA, and hsCRP were significantly higher (P ≤0.001) and TAS significantly lower. Group I and II patients reported a significant reduction in serum levels of MDA and hsCRP and a significant increase in serum levels of TAS, in comparison with pre-treatment levels. There was no significant difference (P = 0.19) in serum MDA levels between groups I and II, whereas, serum TAS levels were significantly higher (P ≤0.01) and hsCRP significantly lower (P ≤0.01) in group II. Conclusion: Acute stroke is associated with oxidative stress and inflammatory response in the early period. G. biloba plays a potential role in reducing oxidative damage and

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“…7) Pharmacological effects of the medicinal plants are related to its free-radical scavenging properties which include inhibition of lipid peroxidation, maintaining integrity and permeability of cell walls [8][9][10] as well as protection of neurons against oxidative stress. 11,12) Roots and rhizomes of Acorus calamus LINN (AC), commonly known as sweet flag, sweet grass and sweet cane (Family: Araceae), have been used in the Indian and Chinese systems of the medicine for hundreds of years for its beneficial role in improved learning performance, and its anti-aging effect. [13][14][15] Shukla et al reported that ethanolic extract of AC prevented acrylamide-induced hind limb paralysis, decreased GSH and GST, increased dopamine receptors in the corpus striatum.…”

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confidence: 99%

Protective Effect of Acorus calamus LINN on Free Radical Scavengers and Lipid Peroxidation in Discrete Regions of Brain against Noise Stress Exposed Rat

Manikandan

Srikumar

Parthasarathy

et al. 2005

Biological & Pharmaceutical Bulletin

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Exposure to continuous loud noise is a serious health problem due to excess production of oxygen free radicals. In medical research, more attention is paid to the antioxidant properties of medicinal plants to minimize the harmful effects of radicals. The aim of this study was to evaluate the protective effect of both ethyl acetate and methanolic extract of Acorus calamus LINN against noise stress (30 d, 100 dBA/4h/d) induced changes in the rat brain. We measured the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the levels of reduced glutathione (GSH), vitamin C, vitamin E, protein thiols and lipid peroxidation (LPO) for the evaluation of oxidative stress status in discrete regions of the rat brain like cerebral cortex, cerebellum, pons-medulla, midbrain, hippocampus and hypothalamus. The results indicated that during exposure of noisy environment ROS generation led to increase in corticosterone, LPO and SOD, but decrease in CAT, GPx, GSH, protein thiols, vitamins C and E levels. Both the ethyl acetate and methanolic extract of Acorus calamus protected most of the changes in the rat brain induced by noise-stress.

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Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide

Cited by 181 publications

References 13 publications

Antioxidant Activity ofRubuscoreanus Fruit Extract: In Comparison to Green Tea Extract

Antioxidant Activity ofRubuscoreanus Fruit Extract: In Comparison to Green Tea Extract

Oxidative Stress and C-Reactive Protein in Patients with Cerebrovascular Accident ( Ischaemic Stroke ) : The Role of Ginkgo Biloba Extract

Protective Effect of Acorus calamus LINN on Free Radical Scavengers and Lipid Peroxidation in Discrete Regions of Brain against Noise Stress Exposed Rat

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